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PsbHLH1, a novel transcription factor involved in regulating anthocyanin biosynthesis in tree peony (Paeonia suffruticosa).
Plant Physiol Biochem. 2020 Jun 12; 154:396-408.PP

Abstract

Flower color is one of the most important features of ornamental plants. Anthocyanin composition and concentration are usually closely related to flower color formation. The biosynthesis of anthocyanin is regulated by a series of structural genes and regulatory genes. The basic helix-loop-helix proteins (bHLHs) are considered as one of the key transcription factors known as the regulators of anthocyanin biosynthesis. However, the bHLH transcription factor family of tree peony (Paeonia suffruticosa) has not been systematically studied in previous studies, especially for the regulation of petal pigmentation. The aim of this study was to identify bHLH genes and unravel their underlying molecular mechanism involved in the regulation of anthocyanin biosynthesis in tree peony. Based on transcriptome profiling analysis, we identified three bHLHs candidate anthocyanin regulators, PsbHLH1, PsbHLH2, and PsbHLH3. PsbHLH1-3 were phylogenetically clustered in the IIIf bHLH subgroup, which is involved in anthocyanin biosynthesis in other plant species. In addition, three bHLH proteins were localized in the nucleus and displayed transcriptional activation activity in a yeast hybrid system. Through a series of functional experiments, we further demonstrated that PsbHLH1 could transcriptionally activate the expression of PsDFR and PsANS via directly binding to their promoters. These results laid a solid foundation to better understand the regulatory mechanisms of anthocyanin biosynthesis in P. suffruticosa and to benefit molecular breeding of tree peony cultivars with novel color.

Authors+Show Affiliations

Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China.Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China.Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China.Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China.Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China.Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China. Electronic address: chwy8915@sina.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32645590

Citation

Qi, Yu, et al. "PsbHLH1, a Novel Transcription Factor Involved in Regulating Anthocyanin Biosynthesis in Tree Peony (Paeonia Suffruticosa)." Plant Physiology and Biochemistry : PPB, vol. 154, 2020, pp. 396-408.
Qi Y, Zhou L, Han L, et al. PsbHLH1, a novel transcription factor involved in regulating anthocyanin biosynthesis in tree peony (Paeonia suffruticosa). Plant Physiol Biochem. 2020;154:396-408.
Qi, Y., Zhou, L., Han, L., Zou, H., Miao, K., & Wang, Y. (2020). PsbHLH1, a novel transcription factor involved in regulating anthocyanin biosynthesis in tree peony (Paeonia suffruticosa). Plant Physiology and Biochemistry : PPB, 154, 396-408. https://doi.org/10.1016/j.plaphy.2020.06.015
Qi Y, et al. PsbHLH1, a Novel Transcription Factor Involved in Regulating Anthocyanin Biosynthesis in Tree Peony (Paeonia Suffruticosa). Plant Physiol Biochem. 2020 Jun 12;154:396-408. PubMed PMID: 32645590.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PsbHLH1, a novel transcription factor involved in regulating anthocyanin biosynthesis in tree peony (Paeonia suffruticosa). AU - Qi,Yu, AU - Zhou,Lin, AU - Han,Lulu, AU - Zou,Hongzhu, AU - Miao,Kun, AU - Wang,Yan, Y1 - 2020/06/12/ PY - 2020/02/11/received PY - 2020/05/12/revised PY - 2020/06/09/accepted PY - 2020/7/10/pubmed PY - 2020/7/10/medline PY - 2020/7/10/entrez KW - ANS KW - DFR KW - Paeonia suffruticosa KW - anthocyanin biosynthesis KW - bHLH transcription factors SP - 396 EP - 408 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol. Biochem. VL - 154 N2 - Flower color is one of the most important features of ornamental plants. Anthocyanin composition and concentration are usually closely related to flower color formation. The biosynthesis of anthocyanin is regulated by a series of structural genes and regulatory genes. The basic helix-loop-helix proteins (bHLHs) are considered as one of the key transcription factors known as the regulators of anthocyanin biosynthesis. However, the bHLH transcription factor family of tree peony (Paeonia suffruticosa) has not been systematically studied in previous studies, especially for the regulation of petal pigmentation. The aim of this study was to identify bHLH genes and unravel their underlying molecular mechanism involved in the regulation of anthocyanin biosynthesis in tree peony. Based on transcriptome profiling analysis, we identified three bHLHs candidate anthocyanin regulators, PsbHLH1, PsbHLH2, and PsbHLH3. PsbHLH1-3 were phylogenetically clustered in the IIIf bHLH subgroup, which is involved in anthocyanin biosynthesis in other plant species. In addition, three bHLH proteins were localized in the nucleus and displayed transcriptional activation activity in a yeast hybrid system. Through a series of functional experiments, we further demonstrated that PsbHLH1 could transcriptionally activate the expression of PsDFR and PsANS via directly binding to their promoters. These results laid a solid foundation to better understand the regulatory mechanisms of anthocyanin biosynthesis in P. suffruticosa and to benefit molecular breeding of tree peony cultivars with novel color. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/32645590/PsbHLH1,_a_novel_transcription_factor_involved_in_regulating_anthocyanin_biosynthesis_in_tree_peony_(Paeonia_suffruticosa) L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(20)30296-5 DB - PRIME DP - Unbound Medicine ER -
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