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The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration.
Cells. 2020 Jul 07; 9(7)C

Abstract

Cells migrating over 2D substrates are required to polymerise actin at the leading edge to form lamellipodia protrusions and nascent adhesions to anchor the protrusion to the substrate. The major actin nucleator in lamellipodia formation is the Arp2/3 complex, which is activated by the WAVE regulatory complex (WRC). Using inducible Nckap1 floxed mouse embryonic fibroblasts (MEFs), we confirm that the WRC is required for lamellipodia formation, and importantly, for generating the retrograde flow of actin from the leading cell edge. The loss of NCKAP1 also affects cell spreading and focal adhesion dynamics. In the absence of lamellipodium, cells can become elongated and move with a single thin pseudopod, which appears devoid of N-WASP. This phenotype was more prevalent on collagen than fibronectin, where we observed an increase in migratory speed. Thus, 2D cell migration on collagen is less dependent on branched actin.

Authors+Show Affiliations

CRUK Beatson Institute, Glasgow G61 1BD, UK.CRUK Beatson Institute, Glasgow G61 1BD, UK. School of Chemistry and Bioscience, University of Bradford, Bradford BD7 1PD, UK.Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth College, Hanover, NH 03755-3844, USA. Zoological Institute, Technische Universität Braunschweig, 38106 Braunschweig, Germany.CRUK Beatson Institute, Glasgow G61 1BD, UK. Institute of Cancer Sciences, University of Glasgow, Glasgow G61 1QH, UK.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32646006

Citation

Whitelaw, Jamie A., et al. "The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration." Cells, vol. 9, no. 7, 2020.
Whitelaw JA, Swaminathan K, Kage F, et al. The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration. Cells. 2020;9(7).
Whitelaw, J. A., Swaminathan, K., Kage, F., & Machesky, L. M. (2020). The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration. Cells, 9(7). https://doi.org/10.3390/cells9071635
Whitelaw JA, et al. The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration. Cells. 2020 Jul 7;9(7) PubMed PMID: 32646006.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration. AU - Whitelaw,Jamie A, AU - Swaminathan,Karthic, AU - Kage,Frieda, AU - Machesky,Laura M, Y1 - 2020/07/07/ PY - 2020/05/31/received PY - 2020/07/02/revised PY - 2020/07/03/accepted PY - 2020/7/11/entrez KW - WAVE complex KW - actin cytoskeleton KW - focal adhesions KW - migration KW - stress fibers JF - Cells JO - Cells VL - 9 IS - 7 N2 - Cells migrating over 2D substrates are required to polymerise actin at the leading edge to form lamellipodia protrusions and nascent adhesions to anchor the protrusion to the substrate. The major actin nucleator in lamellipodia formation is the Arp2/3 complex, which is activated by the WAVE regulatory complex (WRC). Using inducible Nckap1 floxed mouse embryonic fibroblasts (MEFs), we confirm that the WRC is required for lamellipodia formation, and importantly, for generating the retrograde flow of actin from the leading cell edge. The loss of NCKAP1 also affects cell spreading and focal adhesion dynamics. In the absence of lamellipodium, cells can become elongated and move with a single thin pseudopod, which appears devoid of N-WASP. This phenotype was more prevalent on collagen than fibronectin, where we observed an increase in migratory speed. Thus, 2D cell migration on collagen is less dependent on branched actin. SN - 2073-4409 UR - https://www.unboundmedicine.com/medline/citation/32646006/The_WAVE_Regulatory_Complex_Is_Required_to_Balance_Protrusion_and_Adhesion_in_Migration L2 - https://www.mdpi.com/resolver?pii=cells9071635 DB - PRIME DP - Unbound Medicine ER -
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