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[Effect of acupuncture on NLRP3 inflammatory corpuscle in rats with intracerebral hemorrhage].
Zhongguo Zhen Jiu. 2020 Jul 12; 40(7):757-63.ZZ

Abstract

OBJECTIVE

To observe the effect of acupuncture at "Baihui" (GV 20) through "Qubin" (GB 7) on NLRP3 inflammatory corpuscle in rats with intracerebral hemorrhage (ICH), and to explore the action mechanism of acupuncture on promoting the recovery of neural function in rats with ICH.

METHODS

Forty SPF six-week-old male SD rats were randomly divided into a sham operation group, a model group, a non-acupoint group and an acupuncture group, 10 rats in each group. The rats in the model group, non-acupoint group and acupuncture group were intervened with autologous blood injection to prepare ICH model, while the rats in the sham operation group were only intervened with operation but not injection with autologous blood. About 3 hours after the establishment of the model, the rats in the acupuncture group were intervened with acupuncture at "Baihui" (GV 20) through "Qubin" (GB 7), once every 12 hours, for 7 days; the rats in the non-acupoint group were intervened with acupuncture at the non-acupoint [parallel to the "Baihui" (GV 20), 1 cm next to the midline] on the affected side, and other treatment was the same as the acupuncture group. At the end of the intervention, the composite nerve function score of each group was evaluated; the histomorphology of the hemorrhage penumbra was observed by HE staining; the expression of NLRP3 inflammatory corpuscle in the brain was detected by immunohistochemistry; the relative protein expression levels of NLRP3, interleukin-1β (IL-1β) and interleukin-18 (IL-18) in brain were detected by the method of Western blot.

RESULTS

Seven days into intervention, compared with the sham operation group, each item score and total score of composite nerve function in the model group were significantly reduced (P<0.01, P<0.05). There was edema and karyopyknosis in brain neuron as well as necrocytosis and inflammatory cell infiltration in the model group. Compared with the model group and the non-acupoint group, the total score of composite nerve function and the scores of symmetrical movement of limbs (LS) and proprioception of tentacles (VP) in the acupuncture group were increased (P<0.01, P<0.05), and the cell necrosis and inflammatory cell infiltration were relieved. Compared with the sham operation group, NLRP3 inflammatory corpuscle expression and the relative protein expression levels of NLRP3, IL-1β and IL-18 in brain tissue in the model group were increased (P<0.01); compared with the model group and the non-acupoint group, NLRP3 inflammatory corpuscle expression and the relative protein expression levels of NLRP3, IL-1β and IL-18 in brain tissue in the acupuncture group were reduced (P<0.01).

CONCLUSION

Acupuncture at "Baihui" (GV 20) through "Qubin" (GB 7) could downregulate the expression of NLRP3, IL-1β and IL-18 in the brain tissue of ICH rats, inhibit the inflammatory response, and promote the recovery of neural function.

Authors+Show Affiliations

Zhejiang Academy of TCM, Hangzhou 310012, China; Department of Acupuncture-Moxibustion, Tongde Hospital of Zhejiang Province, Hangzhou 310012.Department of Acupuncture-Moxibustion, First Affiliated Hospital of Heilongjiang University of CM.Zhejiang Academy of TCM, Hangzhou 310012, China; Department of Acupuncture-Moxibustion, Tongde Hospital of Zhejiang Province, Hangzhou 310012.Zhejiang Academy of TCM, Hangzhou 310012, China; Department of Acupuncture-Moxibustion, Tongde Hospital of Zhejiang Province, Hangzhou 310012.Zhejiang Academy of TCM, Hangzhou 310012, China; Department of Acupuncture-Moxibustion, Tongde Hospital of Zhejiang Province, Hangzhou 310012.Zhejiang Academy of TCM, Hangzhou 310012, China; Department of Acupuncture-Moxibustion, Tongde Hospital of Zhejiang Province, Hangzhou 310012.Zhejiang Academy of TCM, Hangzhou 310012, China; Department of Acupuncture-Moxibustion, Tongde Hospital of Zhejiang Province, Hangzhou 310012.Zhejiang Academy of TCM, Hangzhou 310012, China; Department of Acupuncture-Moxibustion, Tongde Hospital of Zhejiang Province, Hangzhou 310012.Zhejiang Academy of TCM, Hangzhou 310012, China; Department of Acupuncture-Moxibustion, Tongde Hospital of Zhejiang Province, Hangzhou 310012.

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

32648401

Citation

Liu, Hao, et al. "[Effect of Acupuncture On NLRP3 Inflammatory Corpuscle in Rats With Intracerebral Hemorrhage]." Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, vol. 40, no. 7, 2020, pp. 757-63.
Liu H, Zhang B, Du J, et al. [Effect of acupuncture on NLRP3 inflammatory corpuscle in rats with intracerebral hemorrhage]. Zhongguo Zhen Jiu. 2020;40(7):757-63.
Liu, H., Zhang, B., Du, J., Feng, P. P., Ruan, C., Zhang, W. B., Zhu, Z. H., Zhou, C., & Li, X. W. (2020). [Effect of acupuncture on NLRP3 inflammatory corpuscle in rats with intracerebral hemorrhage]. Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, 40(7), 757-63. https://doi.org/10.13703/j.0255-2930.20190513-k0003
Liu H, et al. [Effect of Acupuncture On NLRP3 Inflammatory Corpuscle in Rats With Intracerebral Hemorrhage]. Zhongguo Zhen Jiu. 2020 Jul 12;40(7):757-63. PubMed PMID: 32648401.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of acupuncture on NLRP3 inflammatory corpuscle in rats with intracerebral hemorrhage]. AU - Liu,Hao, AU - Zhang,Beng, AU - Du,Jia, AU - Feng,Pei-Pei, AU - Ruan,Chen, AU - Zhang,Wei-Bo, AU - Zhu,Zhong-Hua, AU - Zhou,Chi, AU - Li,Xin-Wei, PY - 2020/7/11/entrez KW - NLRP3 inflammatory corpuscle KW - acupuncture KW - composite nerve function score KW - interleukin-18 (IL-18) KW - interleukin-1β (IL-1β) KW - intracerebral hemorrhage (ICH) SP - 757 EP - 63 JF - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JO - Zhongguo Zhen Jiu VL - 40 IS - 7 N2 - OBJECTIVE: To observe the effect of acupuncture at "Baihui" (GV 20) through "Qubin" (GB 7) on NLRP3 inflammatory corpuscle in rats with intracerebral hemorrhage (ICH), and to explore the action mechanism of acupuncture on promoting the recovery of neural function in rats with ICH. METHODS: Forty SPF six-week-old male SD rats were randomly divided into a sham operation group, a model group, a non-acupoint group and an acupuncture group, 10 rats in each group. The rats in the model group, non-acupoint group and acupuncture group were intervened with autologous blood injection to prepare ICH model, while the rats in the sham operation group were only intervened with operation but not injection with autologous blood. About 3 hours after the establishment of the model, the rats in the acupuncture group were intervened with acupuncture at "Baihui" (GV 20) through "Qubin" (GB 7), once every 12 hours, for 7 days; the rats in the non-acupoint group were intervened with acupuncture at the non-acupoint [parallel to the "Baihui" (GV 20), 1 cm next to the midline] on the affected side, and other treatment was the same as the acupuncture group. At the end of the intervention, the composite nerve function score of each group was evaluated; the histomorphology of the hemorrhage penumbra was observed by HE staining; the expression of NLRP3 inflammatory corpuscle in the brain was detected by immunohistochemistry; the relative protein expression levels of NLRP3, interleukin-1β (IL-1β) and interleukin-18 (IL-18) in brain were detected by the method of Western blot. RESULTS: Seven days into intervention, compared with the sham operation group, each item score and total score of composite nerve function in the model group were significantly reduced (P<0.01, P<0.05). There was edema and karyopyknosis in brain neuron as well as necrocytosis and inflammatory cell infiltration in the model group. Compared with the model group and the non-acupoint group, the total score of composite nerve function and the scores of symmetrical movement of limbs (LS) and proprioception of tentacles (VP) in the acupuncture group were increased (P<0.01, P<0.05), and the cell necrosis and inflammatory cell infiltration were relieved. Compared with the sham operation group, NLRP3 inflammatory corpuscle expression and the relative protein expression levels of NLRP3, IL-1β and IL-18 in brain tissue in the model group were increased (P<0.01); compared with the model group and the non-acupoint group, NLRP3 inflammatory corpuscle expression and the relative protein expression levels of NLRP3, IL-1β and IL-18 in brain tissue in the acupuncture group were reduced (P<0.01). CONCLUSION: Acupuncture at "Baihui" (GV 20) through "Qubin" (GB 7) could downregulate the expression of NLRP3, IL-1β and IL-18 in the brain tissue of ICH rats, inhibit the inflammatory response, and promote the recovery of neural function. SN - 0255-2930 UR - https://www.unboundmedicine.com/medline/citation/32648401/[Effect_of_acupuncture_on_NLRP3_inflammatory_corpuscle_in_rats_with_intracerebral_hemorrhage] DB - PRIME DP - Unbound Medicine ER -
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