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Layered Double Hydroxides Are Promising Nanomaterials for Tissue Bioengineering Application.
Adv Biosyst. 2019 07; 3(7):e1800238.AB

Abstract

Layered double hydroxides (LDHs) have emerged as promising nanomaterials for human health and although it has achieved some progress on this matter, their application within bioengineering is not fully addressed. This prompted to subject fibroblasts to two compositions of LDHs (Mg2 Al-Cl and Zn2 Al-Cl), considering an acute response. First, LDH particles are addressed by scanning electron microscopy, and no significant effect of the cell culture medium on the shape of LDHs particles is reported although it seems to adsorb some soluble proteins as proposed by energy-dispersive X-ray analysis. These LDHs release magnesium, zinc, and aluminum, but there is no cytotoxic or biocompatibility effects. The data show interference to fibroblast adhesion by driving the reorganization of actin-based cytoskeleton, preliminarily to cell cycle progression. Additionally, these molecular findings are validated by performing a functional wound-healing assay, which is accompanied by a dynamic extracellular matrix remodeling in response to the LDHs. Altogether, the results show that LDHs nanomaterials modulate cell adhesion, proliferation, and migration, delineating new advances on the biomaterial field applied in the context of soft tissue bioengineering, which must be explored in health disorders, such as wound healing in burn injuries.

Authors+Show Affiliations

Lab. de Bioensaios e Dinâmica Celular, Departamento de Química e Bioquímica, Instituto de Biociências, Universidade Estadual Paulista - UNESP, Campus Botucatu, São Paulo, CEP 18618-970, Brazil.Lab. de Bioensaios e Dinâmica Celular, Departamento de Química e Bioquímica, Instituto de Biociências, Universidade Estadual Paulista - UNESP, Campus Botucatu, São Paulo, CEP 18618-970, Brazil.Lab. de Bioensaios e Dinâmica Celular, Departamento de Química e Bioquímica, Instituto de Biociências, Universidade Estadual Paulista - UNESP, Campus Botucatu, São Paulo, CEP 18618-970, Brazil.Lab. de Bioensaios e Dinâmica Celular, Departamento de Química e Bioquímica, Instituto de Biociências, Universidade Estadual Paulista - UNESP, Campus Botucatu, São Paulo, CEP 18618-970, Brazil.Departamento de Cirurgia, Universidade Federal de São Paulo-UNIFESP, Rua Botucatu 740, CEP 04023-900, São Paulo, SP, Brazil.Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo-USP, Av. Prof. Lineu Prestes 748, CEP 05508-000, São Paulo, SP, Brazil.Lab. de Bioensaios e Dinâmica Celular, Departamento de Química e Bioquímica, Instituto de Biociências, Universidade Estadual Paulista - UNESP, Campus Botucatu, São Paulo, CEP 18618-970, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32648675

Citation

Jr da Costa Fernandes, Célio, et al. "Layered Double Hydroxides Are Promising Nanomaterials for Tissue Bioengineering Application." Advanced Biosystems, vol. 3, no. 7, 2019, pp. e1800238.
Jr da Costa Fernandes C, Pinto TS, Kang HR, et al. Layered Double Hydroxides Are Promising Nanomaterials for Tissue Bioengineering Application. Adv Biosyst. 2019;3(7):e1800238.
Jr da Costa Fernandes, C., Pinto, T. S., Kang, H. R., de Magalhães Padilha, P., Koh, I. H. J., Constantino, V. R. L., & Zambuzzi, W. F. (2019). Layered Double Hydroxides Are Promising Nanomaterials for Tissue Bioengineering Application. Advanced Biosystems, 3(7), e1800238. https://doi.org/10.1002/adbi.201800238
Jr da Costa Fernandes C, et al. Layered Double Hydroxides Are Promising Nanomaterials for Tissue Bioengineering Application. Adv Biosyst. 2019;3(7):e1800238. PubMed PMID: 32648675.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Layered Double Hydroxides Are Promising Nanomaterials for Tissue Bioengineering Application. AU - Jr da Costa Fernandes,Célio, AU - Pinto,Thaís Silva, AU - Kang,Ha Ram, AU - de Magalhães Padilha,Pedro, AU - Koh,Ivan Hong Jun, AU - Constantino,Vera Regina Leopoldo, AU - Zambuzzi,Willian F, Y1 - 2019/05/28/ PY - 2018/08/07/received PY - 2019/05/01/revised PY - 2020/7/11/entrez PY - 2020/7/11/pubmed PY - 2020/7/11/medline KW - bioengineering KW - biofabrication KW - double metal hydroxides KW - fibroblast KW - hydrotalcite-like material KW - layered double hydroxides KW - nanomaterials SP - e1800238 EP - e1800238 JF - Advanced biosystems JO - Adv Biosyst VL - 3 IS - 7 N2 - Layered double hydroxides (LDHs) have emerged as promising nanomaterials for human health and although it has achieved some progress on this matter, their application within bioengineering is not fully addressed. This prompted to subject fibroblasts to two compositions of LDHs (Mg2 Al-Cl and Zn2 Al-Cl), considering an acute response. First, LDH particles are addressed by scanning electron microscopy, and no significant effect of the cell culture medium on the shape of LDHs particles is reported although it seems to adsorb some soluble proteins as proposed by energy-dispersive X-ray analysis. These LDHs release magnesium, zinc, and aluminum, but there is no cytotoxic or biocompatibility effects. The data show interference to fibroblast adhesion by driving the reorganization of actin-based cytoskeleton, preliminarily to cell cycle progression. Additionally, these molecular findings are validated by performing a functional wound-healing assay, which is accompanied by a dynamic extracellular matrix remodeling in response to the LDHs. Altogether, the results show that LDHs nanomaterials modulate cell adhesion, proliferation, and migration, delineating new advances on the biomaterial field applied in the context of soft tissue bioengineering, which must be explored in health disorders, such as wound healing in burn injuries. SN - 2366-7478 UR - https://www.unboundmedicine.com/medline/citation/32648675/Layered_Double_Hydroxides_Are_Promising_Nanomaterials_for_Tissue_Bioengineering_Application DB - PRIME DP - Unbound Medicine ER -
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