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Membrane Poration Mechanisms at the Cell-Nanostructure Interface.
Adv Biosyst. 2019 12; 3(12):e1900148.AB

Abstract

3D vertical nanostructures have become one of the most significant methods for interfacing cells and the nanoscale and for accessing significant intracellular functionalities such as membrane potential. As this intracellular access can be induced by means of diverse cellular membrane poration mechanisms, it is important to investigate in detail the cell condition after membrane rupture for assessing the real effects of the poration techniques on the biological environment. Indeed, differences of the membrane dynamics and reshaping have not been observed yet when the membrane-nanostructure system is locally perturbed by, for instance, diverse membrane breakage events. In this work, new insights are provided into the membrane dynamics in case of two different poration approaches, optoacoustic- and electro-poration, both mediated by the same 3D nanostructures. The experimental results offer a detailed overview on the different poration processes in terms of electrical recordings and membrane conformation.

Authors+Show Affiliations

Istituto Italiano di Tecnologia, Genoa, 16163, Italy.Istituto Italiano di Tecnologia, Genoa, 16163, Italy.Istituto Italiano di Tecnologia, Genoa, 16163, Italy. Dipartimento di Informatica, Bioingegneria, Robotica e Ingegneria dei Sistemi. DIBRIS, Università degli Studi di Genova, Genova, 16126, Italy.Center for Advacend Biomaterials for Healthcare, Istituto Italiano di Tecnologia, Naples, 80125, Italy.Center for Advacend Biomaterials for Healthcare, Istituto Italiano di Tecnologia, Naples, 80125, Italy.Istituto Italiano di Tecnologia, Genoa, 16163, Italy. Dipartimento di Informatica, Bioingegneria, Robotica e Ingegneria dei Sistemi. DIBRIS, Università degli Studi di Genova, Genova, 16126, Italy.Center for Advacend Biomaterials for Healthcare, Istituto Italiano di Tecnologia, Naples, 80125, Italy.Center for Advacend Biomaterials for Healthcare, Istituto Italiano di Tecnologia, Naples, 80125, Italy.Center for Advacend Biomaterials for Healthcare, Istituto Italiano di Tecnologia, Naples, 80125, Italy.Istituto Italiano di Tecnologia, Genoa, 16163, Italy.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32648684

Citation

Dipalo, Michele, et al. "Membrane Poration Mechanisms at the Cell-Nanostructure Interface." Advanced Biosystems, vol. 3, no. 12, 2019, pp. e1900148.
Dipalo M, Caprettini V, Bruno G, et al. Membrane Poration Mechanisms at the Cell-Nanostructure Interface. Adv Biosyst. 2019;3(12):e1900148.
Dipalo, M., Caprettini, V., Bruno, G., Caliendo, F., Garma, L. D., Melle, G., Dukhinova, M., Siciliano, V., Santoro, F., & De Angelis, F. (2019). Membrane Poration Mechanisms at the Cell-Nanostructure Interface. Advanced Biosystems, 3(12), e1900148. https://doi.org/10.1002/adbi.201900148
Dipalo M, et al. Membrane Poration Mechanisms at the Cell-Nanostructure Interface. Adv Biosyst. 2019;3(12):e1900148. PubMed PMID: 32648684.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Membrane Poration Mechanisms at the Cell-Nanostructure Interface. AU - Dipalo,Michele, AU - Caprettini,Valeria, AU - Bruno,Giulia, AU - Caliendo,Fabio, AU - Garma,Leonardo D, AU - Melle,Giovanni, AU - Dukhinova,Marina, AU - Siciliano,Velia, AU - Santoro,Francesca, AU - De Angelis,Francesco, Y1 - 2019/11/06/ PY - 2019/07/11/received PY - 2019/09/21/revised PY - 2020/7/11/entrez PY - 2020/7/11/pubmed PY - 2020/7/11/medline KW - electrophysiology KW - electroporation KW - membrane deformation KW - plasmonic optoporation KW - scanning electron microscopy SP - e1900148 EP - e1900148 JF - Advanced biosystems JO - Adv Biosyst VL - 3 IS - 12 N2 - 3D vertical nanostructures have become one of the most significant methods for interfacing cells and the nanoscale and for accessing significant intracellular functionalities such as membrane potential. As this intracellular access can be induced by means of diverse cellular membrane poration mechanisms, it is important to investigate in detail the cell condition after membrane rupture for assessing the real effects of the poration techniques on the biological environment. Indeed, differences of the membrane dynamics and reshaping have not been observed yet when the membrane-nanostructure system is locally perturbed by, for instance, diverse membrane breakage events. In this work, new insights are provided into the membrane dynamics in case of two different poration approaches, optoacoustic- and electro-poration, both mediated by the same 3D nanostructures. The experimental results offer a detailed overview on the different poration processes in terms of electrical recordings and membrane conformation. SN - 2366-7478 UR - https://www.unboundmedicine.com/medline/citation/32648684/Membrane_Poration_Mechanisms_at_the_Cell-Nanostructure_Interface DB - PRIME DP - Unbound Medicine ER -
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