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High-Content Analysis of Cell Migration Dynamics within a Micropatterned Screening Platform.
Adv Biosyst. 2019 08; 3(8):e1900011.AB

Abstract

Cell migration is a fundamental biological process that is dynamically regulated by complex interactions between the microenvironment and intrinsic gene expression programs. Here, a high-throughput cell migration assay is developed using micropatterned and dynamically adhesive polymer brush substrates, which support highly precise and consistent control over cell-matrix interactions within a 96-well cell culture plate format. This system is combined with automated imaging and quantitation of both cell motility and organization of the F-actin cytoskeleton for high-content analysis of cell migration phenotypes. Using this platform to screen a library of 147 epigenetic inhibitors identifies a set of EZH2-specific compounds that promote cytoskeletal remodeling and accelerates keratinocyte migration through derepression of an epithelial to mesenchymal transition-like gene expression program. Together, these studies establish the high-throughput, micropatterned assay as a powerful tool for discovery of novel therapeutic targets and for dissecting complex gene-environment interactions involved in wound repair.

Authors+Show Affiliations

Centre for Cell Biology and Cutaneous Research, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.Centre for Cell Biology and Cutaneous Research, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.Centre for Cell Biology and Cutaneous Research, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.Centre for Cell Biology and Cutaneous Research, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.Centre for Cell Biology and Cutaneous Research, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.Centre for Cell Biology and Cutaneous Research, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32648701

Citation

Almeida, Filipe V., et al. "High-Content Analysis of Cell Migration Dynamics Within a Micropatterned Screening Platform." Advanced Biosystems, vol. 3, no. 8, 2019, pp. e1900011.
Almeida FV, Gammon L, Laly AC, et al. High-Content Analysis of Cell Migration Dynamics within a Micropatterned Screening Platform. Adv Biosyst. 2019;3(8):e1900011.
Almeida, F. V., Gammon, L., Laly, A. C., Pundel, O. J., Bishop, C. L., & Connelly, J. T. (2019). High-Content Analysis of Cell Migration Dynamics within a Micropatterned Screening Platform. Advanced Biosystems, 3(8), e1900011. https://doi.org/10.1002/adbi.201900011
Almeida FV, et al. High-Content Analysis of Cell Migration Dynamics Within a Micropatterned Screening Platform. Adv Biosyst. 2019;3(8):e1900011. PubMed PMID: 32648701.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-Content Analysis of Cell Migration Dynamics within a Micropatterned Screening Platform. AU - Almeida,Filipe V, AU - Gammon,Luke, AU - Laly,Ana C, AU - Pundel,Oscar J, AU - Bishop,Cleo L, AU - Connelly,John T, Y1 - 2019/06/13/ PY - 2019/01/15/received PY - 2019/05/31/revised PY - 2020/7/11/entrez PY - 2020/7/11/pubmed PY - 2020/7/11/medline KW - EZH2 KW - animal replacement KW - cell migration KW - epigenetics KW - high-throughput SP - e1900011 EP - e1900011 JF - Advanced biosystems JO - Adv Biosyst VL - 3 IS - 8 N2 - Cell migration is a fundamental biological process that is dynamically regulated by complex interactions between the microenvironment and intrinsic gene expression programs. Here, a high-throughput cell migration assay is developed using micropatterned and dynamically adhesive polymer brush substrates, which support highly precise and consistent control over cell-matrix interactions within a 96-well cell culture plate format. This system is combined with automated imaging and quantitation of both cell motility and organization of the F-actin cytoskeleton for high-content analysis of cell migration phenotypes. Using this platform to screen a library of 147 epigenetic inhibitors identifies a set of EZH2-specific compounds that promote cytoskeletal remodeling and accelerates keratinocyte migration through derepression of an epithelial to mesenchymal transition-like gene expression program. Together, these studies establish the high-throughput, micropatterned assay as a powerful tool for discovery of novel therapeutic targets and for dissecting complex gene-environment interactions involved in wound repair. SN - 2366-7478 UR - https://www.unboundmedicine.com/medline/citation/32648701/High-Content_Analysis_of_Cell_Migration_Dynamics_within_a_Micropatterned_Screening_Platform DB - PRIME DP - Unbound Medicine ER -
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