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Detection of Chaetomium globosum, Ch. cochliodes and Ch. rectangulare during the Diversity Tracking of Mycotoxin-Producing Chaetomium-Like Isolates Obtained in Buildings in Finland.
Toxins (Basel). 2020 07 08; 12(7)T

Abstract

The diversity of Chaetomium-like isolates in buildings in Finland is poorly documented. This paper describes a set of methods for rapid diversity tracking of 42 indoor Chaetomium-like isolates. These isolates were categorized based on their fluorescence emission, ascomatal hair morphology, responses in three bioassays and resistance/sensitivity to the wetting agent Genapol X-080. Thirty-nine toxigenic isolates were identified [Ch. globosum (n = 35), Ch. cochliodes (n = 2) and Ch. rectangulare (n = 2)]. These isolates were identified down to the species level by tef1α gene sequencing. The major toxic substances in the ethanol extracts of the Ch. globosum and Ch. cochliodes strains were chaetoglobosin, chaetoviridin A and C, chaetomugilin D and chaetomin, identified based on HPLC-UV and mass spectrometry data (MS and MS/MS). Ethanol extracts from pure Ch. globosum cultures exhibited a toxicological profile in the boar sperm motility inhibition assay (BSMI), sperm membrane integrity damage assay (SMID) and inhibition of cell proliferation (ICP) assay, similar to that exhibited by pure chaetoglobosin A. Overall, differences in fluorescence, morphology, toxicity profile, mycotoxin production and sensitivity to chemicals were consistent with those in tef1α sequencing results for species identification. The results indicate the presence of Ch. cochliodes and Ch. rectangulare in Finnish buildings, representing a new finding.

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Authors+Show Affiliations

Salo JM
Department of Civil Engineering, Aalto University, Box 12100, FI-00076 Aalto, Finland.
Kedves O
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary.
Mikkola R
Department of Civil Engineering, Aalto University, Box 12100, FI-00076 Aalto, Finland.
Kredics L
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary.
Andersson MA
Department of Civil Engineering, Aalto University, Box 12100, FI-00076 Aalto, Finland.
Kurnitski J
Department of Civil Engineering, Aalto University, Box 12100, FI-00076 Aalto, Finland. Department of Civil Engineering and Architecture, Tallinn University of Technology, Ehitajate tee 5, 19086 Tallinn, Estonia.
Salonen H
Department of Civil Engineering, Aalto University, Box 12100, FI-00076 Aalto, Finland.

MeSH

Air MicrobiologyAnimalsCell Line, TumorCell ProliferationChaetomiumDustFinlandMaleMitochondriaMycotoxinsSperm MotilitySpermatozoaSus scrofa

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32650391

Citation

Salo, Johanna M., et al. "Detection of Chaetomium Globosum, Ch. Cochliodes and Ch. Rectangulare During the Diversity Tracking of Mycotoxin-Producing Chaetomium-Like Isolates Obtained in Buildings in Finland." Toxins, vol. 12, no. 7, 2020.
Salo JM, Kedves O, Mikkola R, et al. Detection of Chaetomium globosum, Ch. cochliodes and Ch. rectangulare during the Diversity Tracking of Mycotoxin-Producing Chaetomium-Like Isolates Obtained in Buildings in Finland. Toxins (Basel). 2020;12(7).
Salo, J. M., Kedves, O., Mikkola, R., Kredics, L., Andersson, M. A., Kurnitski, J., & Salonen, H. (2020). Detection of Chaetomium globosum, Ch. cochliodes and Ch. rectangulare during the Diversity Tracking of Mycotoxin-Producing Chaetomium-Like Isolates Obtained in Buildings in Finland. Toxins, 12(7). https://doi.org/10.3390/toxins12070443
Salo JM, et al. Detection of Chaetomium Globosum, Ch. Cochliodes and Ch. Rectangulare During the Diversity Tracking of Mycotoxin-Producing Chaetomium-Like Isolates Obtained in Buildings in Finland. Toxins (Basel). 2020 07 8;12(7) PubMed PMID: 32650391.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Chaetomium globosum, Ch. cochliodes and Ch. rectangulare during the Diversity Tracking of Mycotoxin-Producing Chaetomium-Like Isolates Obtained in Buildings in Finland. AU - Salo,Johanna M, AU - Kedves,Orsolya, AU - Mikkola,Raimo, AU - Kredics,László, AU - Andersson,Maria A, AU - Kurnitski,Jarek, AU - Salonen,Heidi, Y1 - 2020/07/08/ PY - 2020/06/14/received PY - 2020/07/04/revised PY - 2020/07/06/accepted PY - 2020/7/12/entrez PY - 2020/7/12/pubmed PY - 2021/3/3/medline KW - Chaetomium cochliodes KW - Chaetomium globosum KW - Chaetomium rectangulare KW - chaetoglobosin KW - chaetomin KW - chaetomugilin D KW - chaetoviridin A and C KW - fluorescence KW - indoor mold KW - mycotoxin JF - Toxins JO - Toxins (Basel) VL - 12 IS - 7 N2 - The diversity of Chaetomium-like isolates in buildings in Finland is poorly documented. This paper describes a set of methods for rapid diversity tracking of 42 indoor Chaetomium-like isolates. These isolates were categorized based on their fluorescence emission, ascomatal hair morphology, responses in three bioassays and resistance/sensitivity to the wetting agent Genapol X-080. Thirty-nine toxigenic isolates were identified [Ch. globosum (n = 35), Ch. cochliodes (n = 2) and Ch. rectangulare (n = 2)]. These isolates were identified down to the species level by tef1α gene sequencing. The major toxic substances in the ethanol extracts of the Ch. globosum and Ch. cochliodes strains were chaetoglobosin, chaetoviridin A and C, chaetomugilin D and chaetomin, identified based on HPLC-UV and mass spectrometry data (MS and MS/MS). Ethanol extracts from pure Ch. globosum cultures exhibited a toxicological profile in the boar sperm motility inhibition assay (BSMI), sperm membrane integrity damage assay (SMID) and inhibition of cell proliferation (ICP) assay, similar to that exhibited by pure chaetoglobosin A. Overall, differences in fluorescence, morphology, toxicity profile, mycotoxin production and sensitivity to chemicals were consistent with those in tef1α sequencing results for species identification. The results indicate the presence of Ch. cochliodes and Ch. rectangulare in Finnish buildings, representing a new finding. SN - 2072-6651 UR - https://www.unboundmedicine.com/medline/citation/32650391/Detection_of_Chaetomium_globosum_Ch__cochliodes_and_Ch__rectangulare_during_the_Diversity_Tracking_of_Mycotoxin_Producing_Chaetomium_Like_Isolates_Obtained_in_Buildings_in_Finland_ DB - PRIME DP - Unbound Medicine ER -