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Exploring Mechanisms of Quantitative Resistance to Leptosphaeria maculans (Blackleg) in the Cotyledons of Canola (Brassica napus) Based on Transcriptomic and Microscopic Analyses.
Plants (Basel). 2020 Jul 08; 9(7)P

Abstract

Using resistant cultivars is a common approach to managing blackleg of canola/rapeseed caused by Leptosphaeria maculans (Lm). Quantitative resistance (QR), as opposed to major-gene resistance, is of interest because it is generally more durable, due to its multi-genetic basis. However, the mechanisms and genes underlying QR are mostly unknown. In this study, potential QR modes of action in "74‑44 BL" was explored. This Canadian canola cultivar showed moderate but consistent race-nonspecific resistance at the cotyledon and adult-plant stages. A susceptible cultivar, "Westar", was used as a control. After inoculation, the lesions developed more slowly on the cotyledons of 74-44 BL than those of Westar. We used RNA sequencing (-RNA-seq) to identify genes and their functions, putatively related to this resistance, and found that genes involved in programmed cell death (PCD), reactive oxygen species (ROS), signal transduction or intracellular endomembrane transport were most differentially expressed. ROS production was assessed in relation to Lm hyphal growth and lesion size; it occurred beyond the tissue colonized by Lm in 74-44 BL and appeared to trigger rapid cell death, limiting cotyledon colonization by Lm. In contrast, Lm grew more rapidly in Westar, often catching up with the ring of ROS and surpassing lesion boundaries. It appears that QR in 74-44 BL cotyledons is associated with limited colonization by Lm possibly mediated via ROS. The RNA-seq data also showed a link between ROS, signal transduction, and endomembrane vesicle trafficking, as well as PCD in the resistance. These results provide a starting point for a better understanding of the mechanisms behind QR against Lm in canola.

Authors+Show Affiliations

Agriculture and Agri-Food Canada, Swift Current Research and Development Centre, Swift Current, Saskatchewan S9H 3X2, Canada.Agriculture and Agri-Food Canada, Saskatoon Research and Development Centre, Saskatoon, Saskatchewan S7N 0X2, Canada.Agriculture and Agri-Food Canada, Saskatoon Research and Development Centre, Saskatoon, Saskatchewan S7N 0X2, Canada.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32650490

Citation

Hubbard, Michelle, et al. "Exploring Mechanisms of Quantitative Resistance to Leptosphaeria Maculans (Blackleg) in the Cotyledons of Canola (Brassica Napus) Based On Transcriptomic and Microscopic Analyses." Plants (Basel, Switzerland), vol. 9, no. 7, 2020.
Hubbard M, Zhai C, Peng G. Exploring Mechanisms of Quantitative Resistance to Leptosphaeria maculans (Blackleg) in the Cotyledons of Canola (Brassica napus) Based on Transcriptomic and Microscopic Analyses. Plants (Basel). 2020;9(7).
Hubbard, M., Zhai, C., & Peng, G. (2020). Exploring Mechanisms of Quantitative Resistance to Leptosphaeria maculans (Blackleg) in the Cotyledons of Canola (Brassica napus) Based on Transcriptomic and Microscopic Analyses. Plants (Basel, Switzerland), 9(7). https://doi.org/10.3390/plants9070864
Hubbard M, Zhai C, Peng G. Exploring Mechanisms of Quantitative Resistance to Leptosphaeria Maculans (Blackleg) in the Cotyledons of Canola (Brassica Napus) Based On Transcriptomic and Microscopic Analyses. Plants (Basel). 2020 Jul 8;9(7) PubMed PMID: 32650490.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exploring Mechanisms of Quantitative Resistance to Leptosphaeria maculans (Blackleg) in the Cotyledons of Canola (Brassica napus) Based on Transcriptomic and Microscopic Analyses. AU - Hubbard,Michelle, AU - Zhai,Chun, AU - Peng,Gary, Y1 - 2020/07/08/ PY - 2020/05/23/received PY - 2020/06/24/revised PY - 2020/07/06/accepted PY - 2020/7/12/entrez PY - 2020/7/12/pubmed PY - 2020/7/12/medline KW - Leptosphaeria maculans KW - Phoma stem canker KW - RNA-seq KW - ROS KW - canola KW - genetic resistance KW - transcriptome analysis JF - Plants (Basel, Switzerland) JO - Plants (Basel) VL - 9 IS - 7 N2 - Using resistant cultivars is a common approach to managing blackleg of canola/rapeseed caused by Leptosphaeria maculans (Lm). Quantitative resistance (QR), as opposed to major-gene resistance, is of interest because it is generally more durable, due to its multi-genetic basis. However, the mechanisms and genes underlying QR are mostly unknown. In this study, potential QR modes of action in "74‑44 BL" was explored. This Canadian canola cultivar showed moderate but consistent race-nonspecific resistance at the cotyledon and adult-plant stages. A susceptible cultivar, "Westar", was used as a control. After inoculation, the lesions developed more slowly on the cotyledons of 74-44 BL than those of Westar. We used RNA sequencing (-RNA-seq) to identify genes and their functions, putatively related to this resistance, and found that genes involved in programmed cell death (PCD), reactive oxygen species (ROS), signal transduction or intracellular endomembrane transport were most differentially expressed. ROS production was assessed in relation to Lm hyphal growth and lesion size; it occurred beyond the tissue colonized by Lm in 74-44 BL and appeared to trigger rapid cell death, limiting cotyledon colonization by Lm. In contrast, Lm grew more rapidly in Westar, often catching up with the ring of ROS and surpassing lesion boundaries. It appears that QR in 74-44 BL cotyledons is associated with limited colonization by Lm possibly mediated via ROS. The RNA-seq data also showed a link between ROS, signal transduction, and endomembrane vesicle trafficking, as well as PCD in the resistance. These results provide a starting point for a better understanding of the mechanisms behind QR against Lm in canola. SN - 2223-7747 UR - https://www.unboundmedicine.com/medline/citation/32650490/Exploring_Mechanisms_of_Quantitative_Resistance_to_Leptosphaeria_maculans_(Blackleg)_in_the_Cotyledons_of_Canola_(Brassica_napus)_Based_on_Transcriptomic_and_Microscopic_Analyses L2 - https://www.mdpi.com/resolver?pii=plants9070864 DB - PRIME DP - Unbound Medicine ER -
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