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In vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes.
Plants (Basel). 2020 Jul 08; 9(7)P

Abstract

Rapid progress in plant molecular biology in recent years has uncovered the main players in hormonal pathways and characterized transcriptomic networks associated with hormonal response. However, the role of RNA processing, in particular alternative splicing (AS), remains largely unexplored. Here, using example genes involved in cytokinin signaling, brassinosteroid synthesis and auxin transport, we present a set of reporters devised to visualize their AS events in vivo. These reporters show a differential tissue-specific expression of certain transcripts and reveal that expression of some of the them can be changed by the application of the exogenous hormone. Finally, based on the characterized AS event of the PIN7 auxin efflux carrier, we designed a system that allows a rapid genetic screening for the factors upstream of this AS event. Our innovative toolset can be therefore highly useful for exploring novel regulatory nodes of hormonal pathways and potentially helpful for plant researchers focusing on developmental aspects of AS.

Authors+Show Affiliations

Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Czech Academy of Sciences, 165 02 Prague, Czech Republic. Functional Genomics and Proteomics of Plants, Central European Institute of Technology and National Centre for Biomolecular Research, Masaryk University, 625 00 Brno, Czech Republic.Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Czech Academy of Sciences, 165 02 Prague, Czech Republic. Functional Genomics and Proteomics of Plants, Central European Institute of Technology and National Centre for Biomolecular Research, Masaryk University, 625 00 Brno, Czech Republic.Functional Genomics and Proteomics of Plants, Central European Institute of Technology and National Centre for Biomolecular Research, Masaryk University, 625 00 Brno, Czech Republic.Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Czech Academy of Sciences, 165 02 Prague, Czech Republic. Functional Genomics and Proteomics of Plants, Central European Institute of Technology and National Centre for Biomolecular Research, Masaryk University, 625 00 Brno, Czech Republic.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32650629

Citation

Kashkan, Ivan, et al. "In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes." Plants (Basel, Switzerland), vol. 9, no. 7, 2020.
Kashkan I, Timofeyenko K, Kollárová E, et al. In vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes. Plants (Basel). 2020;9(7).
Kashkan, I., Timofeyenko, K., Kollárová, E., & Růžička, K. (2020). In vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes. Plants (Basel, Switzerland), 9(7). https://doi.org/10.3390/plants9070868
Kashkan I, et al. In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes. Plants (Basel). 2020 Jul 8;9(7) PubMed PMID: 32650629.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes. AU - Kashkan,Ivan, AU - Timofeyenko,Ksenia, AU - Kollárová,Eva, AU - Růžička,Kamil, Y1 - 2020/07/08/ PY - 2020/06/10/received PY - 2020/07/02/revised PY - 2020/07/07/accepted PY - 2020/7/12/entrez PY - 2020/7/12/pubmed PY - 2020/7/12/medline KW - AHP6 KW - Arabidopsis KW - D-amino acid oxidase KW - DWF4 KW - PIN7 KW - alternative splicing KW - auxin KW - brassinosteroids KW - cytokinins KW - genetic screen JF - Plants (Basel, Switzerland) JO - Plants (Basel) VL - 9 IS - 7 N2 - Rapid progress in plant molecular biology in recent years has uncovered the main players in hormonal pathways and characterized transcriptomic networks associated with hormonal response. However, the role of RNA processing, in particular alternative splicing (AS), remains largely unexplored. Here, using example genes involved in cytokinin signaling, brassinosteroid synthesis and auxin transport, we present a set of reporters devised to visualize their AS events in vivo. These reporters show a differential tissue-specific expression of certain transcripts and reveal that expression of some of the them can be changed by the application of the exogenous hormone. Finally, based on the characterized AS event of the PIN7 auxin efflux carrier, we designed a system that allows a rapid genetic screening for the factors upstream of this AS event. Our innovative toolset can be therefore highly useful for exploring novel regulatory nodes of hormonal pathways and potentially helpful for plant researchers focusing on developmental aspects of AS. SN - 2223-7747 UR - https://www.unboundmedicine.com/medline/citation/32650629/In_vivo_Reporters_for_Visualizing_Alternative_Splicing_of_Hormonal_Genes L2 - https://www.mdpi.com/resolver?pii=plants9070868 DB - PRIME DP - Unbound Medicine ER -
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