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Analysis of Structural Genomic Diversity in Aegilops umbellulata, Ae. markgrafii, Ae. comosa, and Ae. uniaristata by Fluorescence In Situ Hybridization Karyotyping.
Front Plant Sci. 2020; 11:710.FP

Abstract

Fluorescence in situ hybridization karyotypes have been widely used for evolutionary analysis on chromosome organization and genetic/genomic diversity in the wheat alliance (tribe Triticeae of Poaceae). The karyotpic diversity of Aegilops umbellulata, Ae. markgrafii, Ae. comosa subsp. comosa and subsp. subventricosa, and Ae. uniaristata was evaluated by the fluorescence in situ hybridization (FISH) probes oligo-pSc119.2 and pTa71 in combination with (AAC)5, (ACT)7, and (CTT)12, respectively. Abundant intra- and interspecific genetic variation was discovered in Ae. umbellulata, Ae. markgrafii, and Ae. comosa, but not Ae. uniaristata. Chromosome 7 of Ae. umbellulata had more variants (six variants) than the other six U chromosomes (2-3 variants) as revealed by probes oligo-pSc119.2 and (AAC)5. Intraspecific variation in Ae. markgrafii and Ae. comosa was revealed by oligo-pSc119.2 in combination with (ACT)7 and (CTT)12, respectively. At least five variants were found in every chromosome of Ae. markgrafii and Ae. comosa, and up to 18, 10, and 15 variants were identified for chromosomes 2 of Ae. markgrafii, 4 of Ae. comosa subsp. comosa, and 6 of Ae. comosa subsp. subventricosa. The six Ae. uniaristata accessions showed identical FISH signal patterns. A large number of intra-specific polymorphic FISH signals were observed between the homologous chromosomes of Ae. markgrafii and Ae. comosa, especially chromosomes 1, 2, 4, and 7 of Ae. markgrafii, chromosome 4 of Ae. comosa subsp. comosa, and chromosome 6 of Ae. comosa subsp. subventricosa. Twelve Ae. comosa and 24 Ae. markgrafii accessions showed heteromorphism between homologous chromosomes. Additionally, a translocation between the short arms of chromosomes 1 and 7 of Ae. comosa PI 551038 was identified. The FISH karyotypes can be used to clearly identify the chromosome variations of each chromosome in these Aegilops species and also provide valuable information for understanding the evolutionary relationships and structural genomic variation among Aegilops species.

Authors+Show Affiliations

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang, China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, China.State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang, China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, China.State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang, China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32655588

Citation

Song, Zhongping, et al. "Analysis of Structural Genomic Diversity in Aegilops Umbellulata, Ae. Markgrafii, Ae. Comosa, and Ae. Uniaristata By Fluorescence in Situ Hybridization Karyotyping." Frontiers in Plant Science, vol. 11, 2020, p. 710.
Song Z, Dai S, Bao T, et al. Analysis of Structural Genomic Diversity in Aegilops umbellulata, Ae. markgrafii, Ae. comosa, and Ae. uniaristata by Fluorescence In Situ Hybridization Karyotyping. Front Plant Sci. 2020;11:710.
Song, Z., Dai, S., Bao, T., Zuo, Y., Xiang, Q., Li, J., Liu, G., & Yan, Z. (2020). Analysis of Structural Genomic Diversity in Aegilops umbellulata, Ae. markgrafii, Ae. comosa, and Ae. uniaristata by Fluorescence In Situ Hybridization Karyotyping. Frontiers in Plant Science, 11, 710. https://doi.org/10.3389/fpls.2020.00710
Song Z, et al. Analysis of Structural Genomic Diversity in Aegilops Umbellulata, Ae. Markgrafii, Ae. Comosa, and Ae. Uniaristata By Fluorescence in Situ Hybridization Karyotyping. Front Plant Sci. 2020;11:710. PubMed PMID: 32655588.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of Structural Genomic Diversity in Aegilops umbellulata, Ae. markgrafii, Ae. comosa, and Ae. uniaristata by Fluorescence In Situ Hybridization Karyotyping. AU - Song,Zhongping, AU - Dai,Shoufen, AU - Bao,Tingyu, AU - Zuo,Yuanyuan, AU - Xiang,Qin, AU - Li,Jian, AU - Liu,Gang, AU - Yan,Zehong, Y1 - 2020/06/09/ PY - 2019/12/25/received PY - 2020/05/05/accepted PY - 2020/7/14/entrez PY - 2020/7/14/pubmed PY - 2020/7/14/medline KW - Ae. comosa KW - Ae. markgrafii KW - Ae. umbellulata KW - Ae. uniaristata KW - chromosomal variation KW - diploid Aegilops species KW - fluorescence in situ hybridization (FISH) KW - karyotypes SP - 710 EP - 710 JF - Frontiers in plant science JO - Front Plant Sci VL - 11 N2 - Fluorescence in situ hybridization karyotypes have been widely used for evolutionary analysis on chromosome organization and genetic/genomic diversity in the wheat alliance (tribe Triticeae of Poaceae). The karyotpic diversity of Aegilops umbellulata, Ae. markgrafii, Ae. comosa subsp. comosa and subsp. subventricosa, and Ae. uniaristata was evaluated by the fluorescence in situ hybridization (FISH) probes oligo-pSc119.2 and pTa71 in combination with (AAC)5, (ACT)7, and (CTT)12, respectively. Abundant intra- and interspecific genetic variation was discovered in Ae. umbellulata, Ae. markgrafii, and Ae. comosa, but not Ae. uniaristata. Chromosome 7 of Ae. umbellulata had more variants (six variants) than the other six U chromosomes (2-3 variants) as revealed by probes oligo-pSc119.2 and (AAC)5. Intraspecific variation in Ae. markgrafii and Ae. comosa was revealed by oligo-pSc119.2 in combination with (ACT)7 and (CTT)12, respectively. At least five variants were found in every chromosome of Ae. markgrafii and Ae. comosa, and up to 18, 10, and 15 variants were identified for chromosomes 2 of Ae. markgrafii, 4 of Ae. comosa subsp. comosa, and 6 of Ae. comosa subsp. subventricosa. The six Ae. uniaristata accessions showed identical FISH signal patterns. A large number of intra-specific polymorphic FISH signals were observed between the homologous chromosomes of Ae. markgrafii and Ae. comosa, especially chromosomes 1, 2, 4, and 7 of Ae. markgrafii, chromosome 4 of Ae. comosa subsp. comosa, and chromosome 6 of Ae. comosa subsp. subventricosa. Twelve Ae. comosa and 24 Ae. markgrafii accessions showed heteromorphism between homologous chromosomes. Additionally, a translocation between the short arms of chromosomes 1 and 7 of Ae. comosa PI 551038 was identified. The FISH karyotypes can be used to clearly identify the chromosome variations of each chromosome in these Aegilops species and also provide valuable information for understanding the evolutionary relationships and structural genomic variation among Aegilops species. SN - 1664-462X UR - https://www.unboundmedicine.com/medline/citation/32655588/Analysis_of_Structural_Genomic_Diversity_in_Aegilops_umbellulata_Ae__markgrafii_Ae__comosa_and_Ae__uniaristata_by_Fluorescence_In_Situ_Hybridization_Karyotyping_ L2 - https://doi.org/10.3389/fpls.2020.00710 DB - PRIME DP - Unbound Medicine ER -
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