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Antidiabetic Potency, Antioxidant Effects, and Mode of Actions of Citrus reticulata Fruit Peel Hydroethanolic Extract, Hesperidin, and Quercetin in Nicotinamide/Streptozotocin-Induced Wistar Diabetic Rats.
Oxid Med Cell Longev. 2020; 2020:1730492.OM

Abstract

This study is aimed at assessing the antihyperglycemic, antihyperlipidemic, and antioxidant effects of Citrus reticulata (C. reticulata) fruit peel hydroethanolic extract and two flavonoids, hesperidin and quercetin, in nicotinamide (NA)/streptozotocin- (STZ-) induced type 2 diabetic rats. In addition, GC-MS and HPLC-MS analyses of the extract were performed and the results indicated the presence of multiple flavonoids including hesperidin, quercetin, naringin, and polymethoxylated flavones (nobiletin and tangeretin). To achieve the aim of the study, diabetic rats with NA/STZ-induced T2DM were orally treated with C. reticulata fruit peel hydroethanolic extract, hesperidin, and quercetin at a dose of 100 mg/kg b.w./day for four weeks. The treatments with C. reticulata fruit peel extract, hesperidin, and quercetin significantly ameliorated the impaired oral glucose tolerance; the elevated serum fructosamine level; the diminished serum insulin and C-peptide levels; the altered HOMA-IR, HOMA-IS, and HOMA-β cell function; the decreased liver glycogen content; the increased liver glucose-6-phosphatase and glycogen phosphorylase activities; the deleteriously affected serum lipid profile; the elevated serum AST and ALT activities; and the raised serum creatinine and urea levels in the diabetic rats. The treatments also produced remarkable improvement in the antioxidant defense system manifested by a decrease in the elevated liver lipid peroxidation and an increase in the lowered glutathione content and GPx, GST, and SOD activities. Furthermore, the three treatments enhanced the mRNA expression of GLUT-4 and the insulin receptor β-subunit, but only quercetin produced a significant increase in the expression of adiponectin in adipose tissue of diabetic rats. In conclusion, C. reticulata fruit peel hydroethanolic extract, hesperidin, and quercetin have potent antidiabetic effects which may be mediated through their insulinotropic effects and insulin-sensitizing actions. In addition, the alleviation of the antioxidant defense system by the extract, hesperidin, and naringin may have an important action to enhance the antidiabetic actions and to improve liver and kidney functions in NA/STZ-induced diabetic rats.

Authors+Show Affiliations

Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, P. O. Box 62521, Egypt.Biochemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, P. O. Box 62521, Egypt.Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, P. O. Box 62521, Egypt.Department of Internal Medicine, Faculty of Medicine, Helwan University, Egypt.Department of Zoology, College of Science, King Saud University, P. O. Box 62521, Riyadh 11451, Saudi Arabia. Department of Zoology, Faculty of Science, El-Minia University, P.O. Box 61519, Minya, Egypt.Department of Zoology, College of Science, King Saud University, P. O. Box 62521, Riyadh 11451, Saudi Arabia.Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, P. O. Box 62521, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32655759

Citation

Ali, Alaa M., et al. "Antidiabetic Potency, Antioxidant Effects, and Mode of Actions of Citrus Reticulata Fruit Peel Hydroethanolic Extract, Hesperidin, and Quercetin in Nicotinamide/Streptozotocin-Induced Wistar Diabetic Rats." Oxidative Medicine and Cellular Longevity, vol. 2020, 2020, p. 1730492.
Ali AM, Gabbar MA, Abdel-Twab SM, et al. Antidiabetic Potency, Antioxidant Effects, and Mode of Actions of Citrus reticulata Fruit Peel Hydroethanolic Extract, Hesperidin, and Quercetin in Nicotinamide/Streptozotocin-Induced Wistar Diabetic Rats. Oxid Med Cell Longev. 2020;2020:1730492.
Ali, A. M., Gabbar, M. A., Abdel-Twab, S. M., Fahmy, E. M., Ebaid, H., Alhazza, I. M., & Ahmed, O. M. (2020). Antidiabetic Potency, Antioxidant Effects, and Mode of Actions of Citrus reticulata Fruit Peel Hydroethanolic Extract, Hesperidin, and Quercetin in Nicotinamide/Streptozotocin-Induced Wistar Diabetic Rats. Oxidative Medicine and Cellular Longevity, 2020, 1730492. https://doi.org/10.1155/2020/1730492
Ali AM, et al. Antidiabetic Potency, Antioxidant Effects, and Mode of Actions of Citrus Reticulata Fruit Peel Hydroethanolic Extract, Hesperidin, and Quercetin in Nicotinamide/Streptozotocin-Induced Wistar Diabetic Rats. Oxid Med Cell Longev. 2020;2020:1730492. PubMed PMID: 32655759.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antidiabetic Potency, Antioxidant Effects, and Mode of Actions of Citrus reticulata Fruit Peel Hydroethanolic Extract, Hesperidin, and Quercetin in Nicotinamide/Streptozotocin-Induced Wistar Diabetic Rats. AU - Ali,Alaa M, AU - Gabbar,Mohamed Abdel, AU - Abdel-Twab,Sanaa M, AU - Fahmy,Eman M, AU - Ebaid,Hossam, AU - Alhazza,Ibrahim M, AU - Ahmed,Osama M, Y1 - 2020/06/20/ PY - 2020/01/01/received PY - 2020/05/12/revised PY - 2020/05/23/accepted PY - 2020/7/14/entrez PY - 2020/7/14/pubmed PY - 2021/5/6/medline SP - 1730492 EP - 1730492 JF - Oxidative medicine and cellular longevity JO - Oxid Med Cell Longev VL - 2020 N2 - This study is aimed at assessing the antihyperglycemic, antihyperlipidemic, and antioxidant effects of Citrus reticulata (C. reticulata) fruit peel hydroethanolic extract and two flavonoids, hesperidin and quercetin, in nicotinamide (NA)/streptozotocin- (STZ-) induced type 2 diabetic rats. In addition, GC-MS and HPLC-MS analyses of the extract were performed and the results indicated the presence of multiple flavonoids including hesperidin, quercetin, naringin, and polymethoxylated flavones (nobiletin and tangeretin). To achieve the aim of the study, diabetic rats with NA/STZ-induced T2DM were orally treated with C. reticulata fruit peel hydroethanolic extract, hesperidin, and quercetin at a dose of 100 mg/kg b.w./day for four weeks. The treatments with C. reticulata fruit peel extract, hesperidin, and quercetin significantly ameliorated the impaired oral glucose tolerance; the elevated serum fructosamine level; the diminished serum insulin and C-peptide levels; the altered HOMA-IR, HOMA-IS, and HOMA-β cell function; the decreased liver glycogen content; the increased liver glucose-6-phosphatase and glycogen phosphorylase activities; the deleteriously affected serum lipid profile; the elevated serum AST and ALT activities; and the raised serum creatinine and urea levels in the diabetic rats. The treatments also produced remarkable improvement in the antioxidant defense system manifested by a decrease in the elevated liver lipid peroxidation and an increase in the lowered glutathione content and GPx, GST, and SOD activities. Furthermore, the three treatments enhanced the mRNA expression of GLUT-4 and the insulin receptor β-subunit, but only quercetin produced a significant increase in the expression of adiponectin in adipose tissue of diabetic rats. In conclusion, C. reticulata fruit peel hydroethanolic extract, hesperidin, and quercetin have potent antidiabetic effects which may be mediated through their insulinotropic effects and insulin-sensitizing actions. In addition, the alleviation of the antioxidant defense system by the extract, hesperidin, and naringin may have an important action to enhance the antidiabetic actions and to improve liver and kidney functions in NA/STZ-induced diabetic rats. SN - 1942-0994 UR - https://www.unboundmedicine.com/medline/citation/32655759/Antidiabetic_Potency_Antioxidant_Effects_and_Mode_of_Actions_of_Citrus_reticulata_Fruit_Peel_Hydroethanolic_Extract_Hesperidin_and_Quercetin_in_Nicotinamide/Streptozotocin_Induced_Wistar_Diabetic_Rats_ L2 - https://doi.org/10.1155/2020/1730492 DB - PRIME DP - Unbound Medicine ER -