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A Laboratory Diet-Overlay Bioassay to Monitor Resistance in Lygus lineolaris (Hemiptera: Miridae) to Insecticides Commonly Used in the Mississippi Delta.
J Insect Sci. 2020 Jul 01; 20(4)JI

Abstract

A laboratory, diet-overlay pesticide bioassay was developed using a susceptible population of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), to study its susceptibility to neonicotinoid, sulfoxamine, organophosphate, and pyrethroid insecticides (thiamethoxam, sulfoxaflor, acephate, and permethrin, respectively). The diet-overlay bioassay was compared to the traditional glass-vial surface residue bioassay. We measured LC50 values by feeding tarnished plant bug adults known doses of insecticides dispensed on top of diet in a 10% solution of honey water for thiamethoxam and 10% acetone in water solutions for permethrin, acephate, and sulfoxaflor. Both the diet-overlay and glass-vial bioassays used dose-response (mortality) regression lines to calculate LC50 values for each insecticide at 6-, 24-, 48-, and 72-h post-exposure. Data variability from the glass-vial bioassay was higher for permethrin, sulfoxaflor, and thiamethoxam than the diet-overlay bioassay, for all evaluation times. In contrast, there was lower variability among replicates to acephate in the glass-vial assay compared to the diet-overlay assay. Control mortalities observed on diet-overlay bioassay were lower (0-5%) than those observed on the glass-vial bioassay (4-27%). The use of green beans, floral-foam, rolling glass vials, and insect handling made the existing standard method tedious to manipulate and difficult to handle large numbers of individuals. The nonautoclaved solid diet provides an opportunity to significantly reduce cost and variability associated with procedures of other bioassay methods. In general, the baseline data provide a basis for future comparison to determine changes in resistance over time.

Authors+Show Affiliations

USDA-ARS Southern Insect Management Research Unit, Stoneville, MS.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32658274

Citation

Portilla, Maribel. "A Laboratory Diet-Overlay Bioassay to Monitor Resistance in Lygus Lineolaris (Hemiptera: Miridae) to Insecticides Commonly Used in the Mississippi Delta." Journal of Insect Science (Online), vol. 20, no. 4, 2020.
Portilla M. A Laboratory Diet-Overlay Bioassay to Monitor Resistance in Lygus lineolaris (Hemiptera: Miridae) to Insecticides Commonly Used in the Mississippi Delta. J Insect Sci. 2020;20(4).
Portilla, M. (2020). A Laboratory Diet-Overlay Bioassay to Monitor Resistance in Lygus lineolaris (Hemiptera: Miridae) to Insecticides Commonly Used in the Mississippi Delta. Journal of Insect Science (Online), 20(4). https://doi.org/10.1093/jisesa/ieaa067
Portilla M. A Laboratory Diet-Overlay Bioassay to Monitor Resistance in Lygus Lineolaris (Hemiptera: Miridae) to Insecticides Commonly Used in the Mississippi Delta. J Insect Sci. 2020 Jul 1;20(4) PubMed PMID: 32658274.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Laboratory Diet-Overlay Bioassay to Monitor Resistance in Lygus lineolaris (Hemiptera: Miridae) to Insecticides Commonly Used in the Mississippi Delta. A1 - Portilla,Maribel, PY - 2020/04/22/received PY - 2020/7/14/entrez PY - 2020/7/14/pubmed PY - 2020/12/15/medline KW - diet bioassay KW - insecticide resistance KW - lethal concentration 50 KW - pest monitoring KW - tarnished plant bug JF - Journal of insect science (Online) JO - J Insect Sci VL - 20 IS - 4 N2 - A laboratory, diet-overlay pesticide bioassay was developed using a susceptible population of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), to study its susceptibility to neonicotinoid, sulfoxamine, organophosphate, and pyrethroid insecticides (thiamethoxam, sulfoxaflor, acephate, and permethrin, respectively). The diet-overlay bioassay was compared to the traditional glass-vial surface residue bioassay. We measured LC50 values by feeding tarnished plant bug adults known doses of insecticides dispensed on top of diet in a 10% solution of honey water for thiamethoxam and 10% acetone in water solutions for permethrin, acephate, and sulfoxaflor. Both the diet-overlay and glass-vial bioassays used dose-response (mortality) regression lines to calculate LC50 values for each insecticide at 6-, 24-, 48-, and 72-h post-exposure. Data variability from the glass-vial bioassay was higher for permethrin, sulfoxaflor, and thiamethoxam than the diet-overlay bioassay, for all evaluation times. In contrast, there was lower variability among replicates to acephate in the glass-vial assay compared to the diet-overlay assay. Control mortalities observed on diet-overlay bioassay were lower (0-5%) than those observed on the glass-vial bioassay (4-27%). The use of green beans, floral-foam, rolling glass vials, and insect handling made the existing standard method tedious to manipulate and difficult to handle large numbers of individuals. The nonautoclaved solid diet provides an opportunity to significantly reduce cost and variability associated with procedures of other bioassay methods. In general, the baseline data provide a basis for future comparison to determine changes in resistance over time. SN - 1536-2442 UR - https://www.unboundmedicine.com/medline/citation/32658274/A_Laboratory_Diet_Overlay_Bioassay_to_Monitor_Resistance_in_Lygus_lineolaris__Hemiptera:_Miridae__to_Insecticides_Commonly_Used_in_the_Mississippi_Delta_ DB - PRIME DP - Unbound Medicine ER -