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To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus.
Sci Rep. 2020 07 13; 10(1):11498.SR

Abstract

Chondrus crispus is a marine red alga with sulfated galactans, called carrageenans, in its extracellular matrix. Chondrus has a complex haplodiplontic life cycle, alternating between male and female gametophytes (n) and tetrasporophytes (2n). The Chondrus life cycle stages are isomorphic; however, a major phenotypic difference is that carrageenan composition varies significantly between the tetrasporophytes (mainly lambda-carrageenan) and the gametophytes (mainly kappa/iota-carrageenans). The disparity in carrageenan structures, which confer different chemical properties, strongly suggests differential regulation of carrageenan-active genes between the phases of the Chondrus life cycles. We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France. Transcriptomic analyses reveal differential gene expression of genes encoding several galactose-sulfurylases, carbohydrate-sulfotransferases, glycosyltransferases, and one family 16 glycoside hydrolase. Differential expression of carrageenan-related genes was found primarily between gametophytes and tetrasporophytes, but also between the male and female gametophytes. The differential expression of these multigenic genes provides a rare glimpse into cell wall biosynthesis in algae. Furthermore, it strongly supports that carrageenan metabolism holds an important role in the physiological differentiation between the isomorphic life cycle stages of Chondrus.

Authors+Show Affiliations

Station Biologique de Roscoff, UMR8227, Laboratory of Integrative Biology of Marine Models, CNRS and Sorbonne University, Place Georges Teissier, 29688, Roscoff, France. alipinska@sb-roscoff.fr.Station Biologique de Roscoff, UMR8227, Laboratory of Integrative Biology of Marine Models, CNRS and Sorbonne University, Place Georges Teissier, 29688, Roscoff, France.Department of Biology, The University of Alabama At Birmingham, Campbell Hall 464, 1300 University Blvd, Birmingham, AL, 35294, USA.Station Biologique de Roscoff, UMR8227, Laboratory of Integrative Biology of Marine Models, CNRS and Sorbonne University, Place Georges Teissier, 29688, Roscoff, France.Station Biologique de Roscoff, UMR8227, Laboratory of Integrative Biology of Marine Models, CNRS and Sorbonne University, Place Georges Teissier, 29688, Roscoff, France. efickoblean@sb-roscoff.fr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32661246

Citation

Lipinska, Agnieszka P., et al. "To Gel or Not to Gel: Differential Expression of Carrageenan-related Genes Between the Gametophyte and Tetasporophyte Life Cycle Stages of the Red Alga Chondrus Crispus." Scientific Reports, vol. 10, no. 1, 2020, p. 11498.
Lipinska AP, Collén J, Krueger-Hadfield SA, et al. To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus. Sci Rep. 2020;10(1):11498.
Lipinska, A. P., Collén, J., Krueger-Hadfield, S. A., Mora, T., & Ficko-Blean, E. (2020). To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus. Scientific Reports, 10(1), 11498. https://doi.org/10.1038/s41598-020-67728-6
Lipinska AP, et al. To Gel or Not to Gel: Differential Expression of Carrageenan-related Genes Between the Gametophyte and Tetasporophyte Life Cycle Stages of the Red Alga Chondrus Crispus. Sci Rep. 2020 07 13;10(1):11498. PubMed PMID: 32661246.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - To gel or not to gel: differential expression of carrageenan-related genes between the gametophyte and tetasporophyte life cycle stages of the red alga Chondrus crispus. AU - Lipinska,Agnieszka P, AU - Collén,Jonas, AU - Krueger-Hadfield,Stacy A, AU - Mora,Theo, AU - Ficko-Blean,Elizabeth, Y1 - 2020/07/13/ PY - 2020/02/14/received PY - 2020/05/28/accepted PY - 2020/7/15/entrez PY - 2020/7/15/pubmed PY - 2020/12/17/medline SP - 11498 EP - 11498 JF - Scientific reports JO - Sci Rep VL - 10 IS - 1 N2 - Chondrus crispus is a marine red alga with sulfated galactans, called carrageenans, in its extracellular matrix. Chondrus has a complex haplodiplontic life cycle, alternating between male and female gametophytes (n) and tetrasporophytes (2n). The Chondrus life cycle stages are isomorphic; however, a major phenotypic difference is that carrageenan composition varies significantly between the tetrasporophytes (mainly lambda-carrageenan) and the gametophytes (mainly kappa/iota-carrageenans). The disparity in carrageenan structures, which confer different chemical properties, strongly suggests differential regulation of carrageenan-active genes between the phases of the Chondrus life cycles. We used a combination of taxonomy, biochemistry and molecular biology to characterize the tetrasporophytes and male and female gametophytes from Chondrus individuals isolated from the rocky seashore off the northern coast of France. Transcriptomic analyses reveal differential gene expression of genes encoding several galactose-sulfurylases, carbohydrate-sulfotransferases, glycosyltransferases, and one family 16 glycoside hydrolase. Differential expression of carrageenan-related genes was found primarily between gametophytes and tetrasporophytes, but also between the male and female gametophytes. The differential expression of these multigenic genes provides a rare glimpse into cell wall biosynthesis in algae. Furthermore, it strongly supports that carrageenan metabolism holds an important role in the physiological differentiation between the isomorphic life cycle stages of Chondrus. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/32661246/To_gel_or_not_to_gel:_differential_expression_of_carrageenan_related_genes_between_the_gametophyte_and_tetasporophyte_life_cycle_stages_of_the_red_alga_Chondrus_crispus_ L2 - https://doi.org/10.1038/s41598-020-67728-6 DB - PRIME DP - Unbound Medicine ER -