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Does the human placenta express the canonical cell entry mediators for SARS-CoV-2?
Elife. 2020 07 14; 9E

Abstract

The pandemic of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected more than 10 million people, including pregnant women. To date, no consistent evidence for the vertical transmission of SARS-CoV-2 exists. The novel coronavirus canonically utilizes the angiotensin-converting enzyme 2 (ACE2) receptor and the serine protease TMPRSS2 for cell entry. Herein, building upon our previous single-cell study (Pique-Regi et al., 2019), another study, and new single-cell/nuclei RNA-sequencing data, we investigated the expression of ACE2 and TMPRSS2 throughout pregnancy in the placenta as well as in third-trimester chorioamniotic membranes. We report that co-transcription of ACE2 and TMPRSS2 is negligible in the placenta, thus not a likely path of vertical transmission for SARS-CoV-2. By contrast, receptors for Zika virus and cytomegalovirus, which cause congenital infections, are highly expressed by placental cell types. These data show that the placenta minimally expresses the canonical cell-entry mediators for SARS-CoV-2.

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Authors+Show Affiliations

Pique-Regi R
Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, <italic>Eunice Kennedy Shriver</italic> National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services, Detroit, United States. Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, United States. Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, United States.
Romero R
Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, <italic>Eunice Kennedy Shriver</italic> National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services, Detroit, United States. Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, United States. Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, United States. Department of Epidemiology and Biostatistics, Michigan State University, East Lansing, United States. Detroit Medical Center, Detroit, United States. Department of Obstetrics and Gynecology, Florida International University, Miami, United States.
Tarca AL
Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, <italic>Eunice Kennedy Shriver</italic> National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services, Detroit, United States. Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, United States. Department of Computer Science, Wayne State University College of Engineering, Detroit, United States.
Luca F
Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, United States. Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, United States.
Xu Y
Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, <italic>Eunice Kennedy Shriver</italic> National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services, Detroit, United States. Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, United States.
Alazizi A
Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, United States.
Leng Y
Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, <italic>Eunice Kennedy Shriver</italic> National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services, Detroit, United States. Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, United States.
Hsu CD
Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, <italic>Eunice Kennedy Shriver</italic> National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services, Detroit, United States. Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, United States. Department of Physiology, Wayne State University School of Medicine, Detroit, United States.
Gomez-Lopez N
Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, <italic>Eunice Kennedy Shriver</italic> National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services, Detroit, United States. Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, United States. Department of Biochemistry, Microbiology and Immunology, Wayne State University School of Medicine, Detroit, Michigan, United States.

MeSH

Angiotensin-Converting Enzyme 2BetacoronavirusCOVID-19Coronavirus InfectionsFemaleHumansPandemicsPeptidyl-Dipeptidase APlacentaPneumonia, ViralPregnancyReceptors, VirusSARS-CoV-2Serine EndopeptidasesVirus InternalizationZika VirusZika Virus Infection

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32662421

Citation

Pique-Regi, Roger, et al. "Does the Human Placenta Express the Canonical Cell Entry Mediators for SARS-CoV-2?" ELife, vol. 9, 2020.
Pique-Regi R, Romero R, Tarca AL, et al. Does the human placenta express the canonical cell entry mediators for SARS-CoV-2? Elife. 2020;9.
Pique-Regi, R., Romero, R., Tarca, A. L., Luca, F., Xu, Y., Alazizi, A., Leng, Y., Hsu, C. D., & Gomez-Lopez, N. (2020). Does the human placenta express the canonical cell entry mediators for SARS-CoV-2? ELife, 9. https://doi.org/10.7554/eLife.58716
Pique-Regi R, et al. Does the Human Placenta Express the Canonical Cell Entry Mediators for SARS-CoV-2. Elife. 2020 07 14;9 PubMed PMID: 32662421.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Does the human placenta express the canonical cell entry mediators for SARS-CoV-2? AU - Pique-Regi,Roger, AU - Romero,Roberto, AU - Tarca,Adi L, AU - Luca,Francesca, AU - Xu,Yi, AU - Alazizi,Adnan, AU - Leng,Yaozhu, AU - Hsu,Chaur-Dong, AU - Gomez-Lopez,Nardhy, Y1 - 2020/07/14/ PY - 2020/05/13/received PY - 2020/07/06/accepted PY - 2020/7/15/pubmed PY - 2020/8/1/medline PY - 2020/7/15/entrez KW - COVID-19 KW - SARS-CoV-2 KW - genetics KW - genomics KW - human KW - human biology KW - medicine KW - placenta KW - pregnancy vertical transmisssion KW - scRNA-seq KW - viral receptors JF - eLife JO - Elife VL - 9 N2 - The pandemic of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected more than 10 million people, including pregnant women. To date, no consistent evidence for the vertical transmission of SARS-CoV-2 exists. The novel coronavirus canonically utilizes the angiotensin-converting enzyme 2 (ACE2) receptor and the serine protease TMPRSS2 for cell entry. Herein, building upon our previous single-cell study (Pique-Regi et al., 2019), another study, and new single-cell/nuclei RNA-sequencing data, we investigated the expression of ACE2 and TMPRSS2 throughout pregnancy in the placenta as well as in third-trimester chorioamniotic membranes. We report that co-transcription of ACE2 and TMPRSS2 is negligible in the placenta, thus not a likely path of vertical transmission for SARS-CoV-2. By contrast, receptors for Zika virus and cytomegalovirus, which cause congenital infections, are highly expressed by placental cell types. These data show that the placenta minimally expresses the canonical cell-entry mediators for SARS-CoV-2. SN - 2050-084X UR - https://www.unboundmedicine.com/medline/citation/32662421/Does_the_human_placenta_express_the_canonical_cell_entry_mediators_for_SARS_CoV_2 DB - PRIME DP - Unbound Medicine ER -