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Platelet-rich plasma inhibits osteoblast apoptosis and actin cytoskeleton disruption induced by gingipains through upregulating integrin β1.
Cell Biol Int. 2020 Jul 14 [Online ahead of print]CB

Abstract

The aim of this study was to explore the effects of platelet-rich plasma on gingipain-caused changes in cell morphology and apoptosis of osteoblasts. Mouse osteoblasts MC3T3-E1 cells were treated with gingipain extracts from Porphyromonas gingivalis in the presence or absence of platelet-rich plasma. Apoptosis was detected with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling staining. F-actin was determined by phalloidin-fluorescent staining and observed under confocal microscopy. Western blot analysis was used to detect integrin β1, F-actin, and G-actin protein expressions. A knocking down approach was used to determine the role of integrin β1. The platelet-rich plasma protected osteoblasts from gingipain-induced apoptosis in a dose-dependent manner, accompanied by upregulation of integrin β1. Platelet-rich plasma reversed the loss of F-actin integrity and decrease of F-actin/G-actin ratio in osteoblasts in the presence of gingipains. By contrast, the effects of platelet-rich plasma were abrogated by knockdown of integrin β1. The platelet-rich plasma failed to reduce cell apoptosis and reorganize the cytoskeleton after knockdown of integrin β1. In conclusion, platelet-rich plasma inhibits gingipain-induced osteoblast apoptosis and actin cytoskeleton disruption by upregulating integrin β1 expression.

Authors+Show Affiliations

Department of Periodontology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, China. The Stomatology Medical Center, The First People's Hospital of Foshan, Foshan, China.Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, China.Department of Periodontology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, China. Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.Department of Periodontology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, China. Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.Department of Periodontology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, China. Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, China.Department of Periodontology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, China. Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.Department of Periodontology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, China. Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32662922

Citation

Mo, Weiyan, et al. "Platelet-rich Plasma Inhibits Osteoblast Apoptosis and Actin Cytoskeleton Disruption Induced By Gingipains Through Upregulating Integrin Β1." Cell Biology International, 2020.
Mo W, Wu J, Qiu Q, et al. Platelet-rich plasma inhibits osteoblast apoptosis and actin cytoskeleton disruption induced by gingipains through upregulating integrin β1. Cell Biol Int. 2020.
Mo, W., Wu, J., Qiu, Q., Zhang, F., Luo, H., Xu, N., Zhu, W., & Liang, M. (2020). Platelet-rich plasma inhibits osteoblast apoptosis and actin cytoskeleton disruption induced by gingipains through upregulating integrin β1. Cell Biology International. https://doi.org/10.1002/cbin.11420
Mo W, et al. Platelet-rich Plasma Inhibits Osteoblast Apoptosis and Actin Cytoskeleton Disruption Induced By Gingipains Through Upregulating Integrin Β1. Cell Biol Int. 2020 Jul 14; PubMed PMID: 32662922.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Platelet-rich plasma inhibits osteoblast apoptosis and actin cytoskeleton disruption induced by gingipains through upregulating integrin β1. AU - Mo,Weiyan, AU - Wu,Juan, AU - Qiu,Qihong, AU - Zhang,Fuping, AU - Luo,Haoyuan, AU - Xu,Na, AU - Zhu,Wenjun, AU - Liang,Min, Y1 - 2020/07/14/ PY - 2020/02/29/received PY - 2020/07/02/revised PY - 2020/07/12/accepted PY - 2020/7/15/pubmed PY - 2020/7/15/medline PY - 2020/7/15/entrez KW - F-actin KW - cell death KW - gingipains KW - integrin β1 KW - periodontitis KW - platelet-rich plasma JF - Cell biology international JO - Cell Biol. Int. N2 - The aim of this study was to explore the effects of platelet-rich plasma on gingipain-caused changes in cell morphology and apoptosis of osteoblasts. Mouse osteoblasts MC3T3-E1 cells were treated with gingipain extracts from Porphyromonas gingivalis in the presence or absence of platelet-rich plasma. Apoptosis was detected with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling staining. F-actin was determined by phalloidin-fluorescent staining and observed under confocal microscopy. Western blot analysis was used to detect integrin β1, F-actin, and G-actin protein expressions. A knocking down approach was used to determine the role of integrin β1. The platelet-rich plasma protected osteoblasts from gingipain-induced apoptosis in a dose-dependent manner, accompanied by upregulation of integrin β1. Platelet-rich plasma reversed the loss of F-actin integrity and decrease of F-actin/G-actin ratio in osteoblasts in the presence of gingipains. By contrast, the effects of platelet-rich plasma were abrogated by knockdown of integrin β1. The platelet-rich plasma failed to reduce cell apoptosis and reorganize the cytoskeleton after knockdown of integrin β1. In conclusion, platelet-rich plasma inhibits gingipain-induced osteoblast apoptosis and actin cytoskeleton disruption by upregulating integrin β1 expression. SN - 1095-8355 UR - https://www.unboundmedicine.com/medline/citation/32662922/Platelet_Rich_Plasma_Inhibits_Osteoblast_Apoptosis_and_Actin_Cytoskeleton_Disruption_Induced_by_Gingipains_through_Upregulating_Integrin_β1 L2 - https://doi.org/10.1002/cbin.11420 DB - PRIME DP - Unbound Medicine ER -
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