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Genome Wide Association Mapping of Spot Blotch Resistance at Seedling and Adult Plant Stages in Barley.
Front Plant Sci. 2020; 11:642.FP

Abstract

Barley spot blotch (SB) caused by Cochliobolus sativus is one of the major constrains to barley production in warmer regions worldwide. The study was undertaken to identify and estimate effects of loci underlying quantitative resistance to SB at the seedling and adult plant stages. A panel of 261 high input (HI-AM) barley genotypes consisting of released cultivars, advanced breeding lines, and landraces, was screened for resistance to SB. The seedling resistance screening was conducted using two virulent isolates from Morocco (ICSB3 and SB54) while the adult plant stage resistance was evaluated at two hot spot locations, Faizabad and Varanasi, in India under artificial inoculation using a mixture of prevalent virulent isolates. The HI-AM panel was genotyped using DArT-Seq high-throughput genotyping platform. Genome wide association mapping (GWAM) was conducted using 13,182 PAV and 6,311 SNP markers, for seedling and adult plant resistance. Both GLM and MLM model were employed in TASSEL (v 5.0) using principal component analysis and Kinship Matrix as covariates. Final disease rating and Area Under Disease Progress Curve (AUDPC) were used for the evaluation of adult stage plant resistance. The GWAM analysis indicated 23 QTL at the seedling stage (14 for isolate ICSB3 and 9 for isolate SB54), while 15 QTL were detected at the adult plant stage resistance (6 at Faizabad and 9 at Varanasi) and 5 for AUDPC based resistance at Varanasi. Common QTL at seedling and adult plant stages were found across all barley chromosomes. Seedling stage QTL explained together 73.24% of the variance for seedling resistance to isolate ICSB3 and 49.26% for isolate SB54, whereas, QTL for adult plant stage resistance explained together 38.32%, 44.09% and 26.42% of the variance at Faizabad and Varanasi and AUDPC at Varanasi, respectively. Several QTL identified in this study were also reported in previous studies using bi-parental and association mapping populations, corroborating our results. The promising QTL detected at both stages, once validated, can be used for marker assisted selection (MAS) in SB resistance barley breeding program.

Authors+Show Affiliations

Biodiversity and Crop Improvement Program, International Center for Agricultural Research in the Dry Areas, Rabat, Morocco.Biodiversity and Crop Improvement Program, International Center for Agricultural Research in the Dry Areas, Rabat, Morocco.Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India.Department of Plant Pathology, Narendra Dev University of Agriculture and Technology, Faizabad, India.Department of Plant Pathology, Narendra Dev University of Agriculture and Technology, Faizabad, India.Biodiversity and Crop Improvement Program, International Center for Agricultural Research in the Dry Areas, Rabat, Morocco. Vegetable Seed Pathology Department, Washington State University, Northwest Washington Research and Extension Center, Mount Vernon, WA, United States.Biodiversity and Crop Improvement Program, International Center for Agricultural Research in the Dry Areas, Rabat, Morocco. Department of Horticulture and Crop Science, Faculty of Agriculture, The University of Jordan, Amman, Jordan.Biodiversity and Crop Improvement Program, International Center for Agricultural Research in the Dry Areas, Rabat, Morocco.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32670303

Citation

Visioni, Andrea, et al. "Genome Wide Association Mapping of Spot Blotch Resistance at Seedling and Adult Plant Stages in Barley." Frontiers in Plant Science, vol. 11, 2020, p. 642.
Visioni A, Rehman S, Viash SS, et al. Genome Wide Association Mapping of Spot Blotch Resistance at Seedling and Adult Plant Stages in Barley. Front Plant Sci. 2020;11:642.
Visioni, A., Rehman, S., Viash, S. S., Singh, S. P., Vishwakarma, R., Gyawali, S., Al-Abdallat, A. M., & Verma, R. P. S. (2020). Genome Wide Association Mapping of Spot Blotch Resistance at Seedling and Adult Plant Stages in Barley. Frontiers in Plant Science, 11, 642. https://doi.org/10.3389/fpls.2020.00642
Visioni A, et al. Genome Wide Association Mapping of Spot Blotch Resistance at Seedling and Adult Plant Stages in Barley. Front Plant Sci. 2020;11:642. PubMed PMID: 32670303.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome Wide Association Mapping of Spot Blotch Resistance at Seedling and Adult Plant Stages in Barley. AU - Visioni,Andrea, AU - Rehman,Sajid, AU - Viash,Shyam Saran, AU - Singh,Shiw Pratap, AU - Vishwakarma,Ram, AU - Gyawali,Sanjaya, AU - Al-Abdallat,Ayed M, AU - Verma,Ramesh Pal Singh, Y1 - 2020/05/25/ PY - 2019/07/04/received PY - 2020/04/24/accepted PY - 2020/7/17/entrez PY - 2020/7/17/pubmed PY - 2020/7/17/medline KW - AUDPC KW - Cochliobolus KW - GWAM KW - barley KW - resistance KW - spot blotch SP - 642 EP - 642 JF - Frontiers in plant science JO - Front Plant Sci VL - 11 N2 - Barley spot blotch (SB) caused by Cochliobolus sativus is one of the major constrains to barley production in warmer regions worldwide. The study was undertaken to identify and estimate effects of loci underlying quantitative resistance to SB at the seedling and adult plant stages. A panel of 261 high input (HI-AM) barley genotypes consisting of released cultivars, advanced breeding lines, and landraces, was screened for resistance to SB. The seedling resistance screening was conducted using two virulent isolates from Morocco (ICSB3 and SB54) while the adult plant stage resistance was evaluated at two hot spot locations, Faizabad and Varanasi, in India under artificial inoculation using a mixture of prevalent virulent isolates. The HI-AM panel was genotyped using DArT-Seq high-throughput genotyping platform. Genome wide association mapping (GWAM) was conducted using 13,182 PAV and 6,311 SNP markers, for seedling and adult plant resistance. Both GLM and MLM model were employed in TASSEL (v 5.0) using principal component analysis and Kinship Matrix as covariates. Final disease rating and Area Under Disease Progress Curve (AUDPC) were used for the evaluation of adult stage plant resistance. The GWAM analysis indicated 23 QTL at the seedling stage (14 for isolate ICSB3 and 9 for isolate SB54), while 15 QTL were detected at the adult plant stage resistance (6 at Faizabad and 9 at Varanasi) and 5 for AUDPC based resistance at Varanasi. Common QTL at seedling and adult plant stages were found across all barley chromosomes. Seedling stage QTL explained together 73.24% of the variance for seedling resistance to isolate ICSB3 and 49.26% for isolate SB54, whereas, QTL for adult plant stage resistance explained together 38.32%, 44.09% and 26.42% of the variance at Faizabad and Varanasi and AUDPC at Varanasi, respectively. Several QTL identified in this study were also reported in previous studies using bi-parental and association mapping populations, corroborating our results. The promising QTL detected at both stages, once validated, can be used for marker assisted selection (MAS) in SB resistance barley breeding program. SN - 1664-462X UR - https://www.unboundmedicine.com/medline/citation/32670303/Genome_Wide_Association_Mapping_of_Spot_Blotch_Resistance_at_Seedling_and_Adult_Plant_Stages_in_Barley_ L2 - https://doi.org/10.3389/fpls.2020.00642 DB - PRIME DP - Unbound Medicine ER -
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