Tags

Type your tag names separated by a space and hit enter

Evaluation of a six-dye multiplex composed of 27 markers for forensic analysis and databasing.
Mol Genet Genomic Med. 2020 09; 8(9):e1419.MG

Abstract

BACKGROUND

Short tandem repeat (STR) markers play a significant role in genetic applications and have proved to be effective for the personal identification in forensic medicine. In this study, a six-dye multiplex composed of 23 autosomal STR loci (TH01, D3S1358, Penta D, D6S1043, D21S11, TPOX, D1S1656, D12S391, Penta E, D10S1248, D22S1045, D19S433, D8S1179, D2S1338, D2S441, D18S51, vWA, FGA, D16S539, CSF1PO, D13S317, D5S818, D7S820), one Y chromosome STR (DYS391), two internal quality control markers (Quality Sensor QS1 and QS2), and Amelogenin was evaluated.

METHODS

Evaluation studies, including PCR-based studies, sensitivity studies, species specificity studies, stability studies, DNA mixture studies, concordance studies, and precision evaluations were performed according to the guidelines of "Validation Guidelines for Forensic DNA Analysis Methods (2016)" by the Scientific Working Group on DNA Analysis Methods (SWGDAM). In addition, the forensic characteristics of 357 unrelated male samples from Han and Hui populations in China were investigated using 27 markers.

RESULTS

Full STR profiles were obtained from different reaction volumes (5 ~ 25 μl), cycle numbers (28 ~ 34 cycles) and annealing temperatures (58 ~ 62°C). All STR profiles were obtained at humic acid concentration of up to 200 ng/μl and hematin concentration of up to 500 μM. No peaks were observed in most common animal samples except two innovative internal PCR controls (Quality Sensor QS1 and QS2). The six-dye multiplex showed a notably high value for the combined probability of exclusion (CPE), exhibiting values of with 0.99999999977688 in the Han population and 0.999999999583875 in the Hui population. The values of combined probability of discrimination (CPD) were 0.999999999999999999999999999997453 in the Han population and 0. 999999999999999999999999999994398 in the Hui population. In addition, concordance studies showed that there was no difference with the AGCU Express Marker 22 Kit.

CONCLUSION

The results indicated that the Investigator® 26plex QS Kit is a robust, reliable, and suitable tool for forensic analysis and databasing.

Authors+Show Affiliations

Department of Forensic Genetics, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, China.Department of Forensic Genetics, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, China.Department of Obstetrics, Xiangya Hospital, Central South University, Changsha, China.Department of Forensic Genetics, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, China.Department of Forensic Genetics, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, China.Department of Criminal Investigation Bureau of Sichuan Provincial Public Security, Chengdu, China.Department of Forensic Genetics, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, China. Key Laboratory of Sichuan Higher Education, Criminal Science and Technology Laboratory, Sichuan Police College, Luzhou, China.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32677357

Citation

Wang, Shuangshuang, et al. "Evaluation of a Six-dye Multiplex Composed of 27 Markers for Forensic Analysis and Databasing." Molecular Genetics & Genomic Medicine, vol. 8, no. 9, 2020, pp. e1419.
Wang S, Song F, Xie M, et al. Evaluation of a six-dye multiplex composed of 27 markers for forensic analysis and databasing. Mol Genet Genomic Med. 2020;8(9):e1419.
Wang, S., Song, F., Xie, M., Zhang, K., Xie, B., Huang, Z., & Luo, H. (2020). Evaluation of a six-dye multiplex composed of 27 markers for forensic analysis and databasing. Molecular Genetics & Genomic Medicine, 8(9), e1419. https://doi.org/10.1002/mgg3.1419
Wang S, et al. Evaluation of a Six-dye Multiplex Composed of 27 Markers for Forensic Analysis and Databasing. Mol Genet Genomic Med. 2020;8(9):e1419. PubMed PMID: 32677357.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of a six-dye multiplex composed of 27 markers for forensic analysis and databasing. AU - Wang,Shuangshuang, AU - Song,Feng, AU - Xie,Mingkun, AU - Zhang,Ke, AU - Xie,Bowen, AU - Huang,Zhanglong, AU - Luo,Haibo, Y1 - 2020/07/16/ PY - 2020/05/07/received PY - 2020/06/10/revised PY - 2020/07/01/accepted PY - 2020/7/18/pubmed PY - 2021/5/28/medline PY - 2020/7/18/entrez KW - concordance study KW - forensic science KW - mixture study KW - sensitivity KW - short tandem repeat (STR) SP - e1419 EP - e1419 JF - Molecular genetics & genomic medicine JO - Mol Genet Genomic Med VL - 8 IS - 9 N2 - BACKGROUND: Short tandem repeat (STR) markers play a significant role in genetic applications and have proved to be effective for the personal identification in forensic medicine. In this study, a six-dye multiplex composed of 23 autosomal STR loci (TH01, D3S1358, Penta D, D6S1043, D21S11, TPOX, D1S1656, D12S391, Penta E, D10S1248, D22S1045, D19S433, D8S1179, D2S1338, D2S441, D18S51, vWA, FGA, D16S539, CSF1PO, D13S317, D5S818, D7S820), one Y chromosome STR (DYS391), two internal quality control markers (Quality Sensor QS1 and QS2), and Amelogenin was evaluated. METHODS: Evaluation studies, including PCR-based studies, sensitivity studies, species specificity studies, stability studies, DNA mixture studies, concordance studies, and precision evaluations were performed according to the guidelines of "Validation Guidelines for Forensic DNA Analysis Methods (2016)" by the Scientific Working Group on DNA Analysis Methods (SWGDAM). In addition, the forensic characteristics of 357 unrelated male samples from Han and Hui populations in China were investigated using 27 markers. RESULTS: Full STR profiles were obtained from different reaction volumes (5 ~ 25 μl), cycle numbers (28 ~ 34 cycles) and annealing temperatures (58 ~ 62°C). All STR profiles were obtained at humic acid concentration of up to 200 ng/μl and hematin concentration of up to 500 μM. No peaks were observed in most common animal samples except two innovative internal PCR controls (Quality Sensor QS1 and QS2). The six-dye multiplex showed a notably high value for the combined probability of exclusion (CPE), exhibiting values of with 0.99999999977688 in the Han population and 0.999999999583875 in the Hui population. The values of combined probability of discrimination (CPD) were 0.999999999999999999999999999997453 in the Han population and 0. 999999999999999999999999999994398 in the Hui population. In addition, concordance studies showed that there was no difference with the AGCU Express Marker 22 Kit. CONCLUSION: The results indicated that the Investigator® 26plex QS Kit is a robust, reliable, and suitable tool for forensic analysis and databasing. SN - 2324-9269 UR - https://www.unboundmedicine.com/medline/citation/32677357/Evaluation_of_a_six_dye_multiplex_composed_of_27_markers_for_forensic_analysis_and_databasing_ L2 - https://doi.org/10.1002/mgg3.1419 DB - PRIME DP - Unbound Medicine ER -