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Screening of 104 New Psychoactive Substances (NPS) and Other Drugs of Abuse in Oral Fluid by LC-MS-MS.
J Anal Toxicol. 2020 Oct 12; 44(7):697-707.JA

Abstract

New psychoactive substances (NPS) are a major public health problem, primarily due to the increased number of acute poisoning cases. Detection of these substances is a challenge. The aim of this research was to develop and validate a sensitive screening method for 104 drugs of abuse, including synthetic cannabinoids, synthetic cathinones, fentanyl analogues, phenethylamines and other abused psychoactive compounds (i.e., THC, MDMA, LSD and their metabolites) in oral fluid by liquid chromatography-tandem mass spectrometry (LC-MS-MS). The Quantisal™ oral fluid device was used to collect oral fluid samples. The oral fluid-elution buffer mixture (500-μL sample) was extracted with t-butyl methyl ether, and chromatographic separation was performed on a Raptor™ biphenyl column (100 × 2.1 mm ID, 2.7 μm), with a total run time of 13.5 min. Limits of detection were established at three concentrations (0.05, 0.1 or 1 ng/mL) for most analytes, except for acetyl norfentanyl and mescaline (5 ng/mL). Matrix effects were generally <20% and overall extraction recoveries >60%. The highest matrix effect was observed within the synthetic cannabinoid group (PB22, -55.5%). Lower recoveries were observed for 2C-T (47.2%) and JWH-175 (58.7%). Recoveries from the Quantisal™ device were also evaluated for all analytes (56.7-127%), with lower recoveries noted for 25I-NBOMe, valerylfentanyl and mCPP (56.7, 63.0 and 69.9%, respectively). Drug stability in oral fluid was evaluated at 15, 60 and 90 days and at 25, 4 and -20°C. As expected, greater stability was observed when samples were stored at -20°C, but even when frozen, some NPS (e.g., synthetic cannabinoids) showed more than 20% degradation. The method was successfully applied to the analysis of seven authentic oral fluid samples positive for 17 different analytes. The method achieved good sensitivity and simultaneous detection of a wide range of NPS.

Authors+Show Affiliations

Faculty of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP 13083-859, Brazil. Campinas Poison Control Center, University of Campinas (UNICAMP), Campinas, SP 13083-859, Brazil.Faculty of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP 13083-859, Brazil. Campinas Poison Control Center, University of Campinas (UNICAMP), Campinas, SP 13083-859, Brazil.Institute of Emerging Health Professions, Thomas Jefferson University, Philadelphia, PA 19107, USA.Campinas Poison Control Center, University of Campinas (UNICAMP), Campinas, SP 13083-859, Brazil. Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), Campinas, SP 13083-859, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32685960

Citation

da Cunha, Kelly Francisco, et al. "Screening of 104 New Psychoactive Substances (NPS) and Other Drugs of Abuse in Oral Fluid By LC-MS-MS." Journal of Analytical Toxicology, vol. 44, no. 7, 2020, pp. 697-707.
da Cunha KF, Oliveira KD, Huestis MA, et al. Screening of 104 New Psychoactive Substances (NPS) and Other Drugs of Abuse in Oral Fluid by LC-MS-MS. J Anal Toxicol. 2020;44(7):697-707.
da Cunha, K. F., Oliveira, K. D., Huestis, M. A., & Costa, J. L. (2020). Screening of 104 New Psychoactive Substances (NPS) and Other Drugs of Abuse in Oral Fluid by LC-MS-MS. Journal of Analytical Toxicology, 44(7), 697-707. https://doi.org/10.1093/jat/bkaa089
da Cunha KF, et al. Screening of 104 New Psychoactive Substances (NPS) and Other Drugs of Abuse in Oral Fluid By LC-MS-MS. J Anal Toxicol. 2020 Oct 12;44(7):697-707. PubMed PMID: 32685960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Screening of 104 New Psychoactive Substances (NPS) and Other Drugs of Abuse in Oral Fluid by LC-MS-MS. AU - da Cunha,Kelly Francisco, AU - Oliveira,Karina Diniz, AU - Huestis,Marilyn A, AU - Costa,Jose Luiz, PY - 2020/02/14/received PY - 2020/06/22/revised PY - 2020/07/10/accepted PY - 2020/7/21/pubmed PY - 2020/10/21/medline PY - 2020/7/21/entrez SP - 697 EP - 707 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 44 IS - 7 N2 - New psychoactive substances (NPS) are a major public health problem, primarily due to the increased number of acute poisoning cases. Detection of these substances is a challenge. The aim of this research was to develop and validate a sensitive screening method for 104 drugs of abuse, including synthetic cannabinoids, synthetic cathinones, fentanyl analogues, phenethylamines and other abused psychoactive compounds (i.e., THC, MDMA, LSD and their metabolites) in oral fluid by liquid chromatography-tandem mass spectrometry (LC-MS-MS). The Quantisal™ oral fluid device was used to collect oral fluid samples. The oral fluid-elution buffer mixture (500-μL sample) was extracted with t-butyl methyl ether, and chromatographic separation was performed on a Raptor™ biphenyl column (100 × 2.1 mm ID, 2.7 μm), with a total run time of 13.5 min. Limits of detection were established at three concentrations (0.05, 0.1 or 1 ng/mL) for most analytes, except for acetyl norfentanyl and mescaline (5 ng/mL). Matrix effects were generally <20% and overall extraction recoveries >60%. The highest matrix effect was observed within the synthetic cannabinoid group (PB22, -55.5%). Lower recoveries were observed for 2C-T (47.2%) and JWH-175 (58.7%). Recoveries from the Quantisal™ device were also evaluated for all analytes (56.7-127%), with lower recoveries noted for 25I-NBOMe, valerylfentanyl and mCPP (56.7, 63.0 and 69.9%, respectively). Drug stability in oral fluid was evaluated at 15, 60 and 90 days and at 25, 4 and -20°C. As expected, greater stability was observed when samples were stored at -20°C, but even when frozen, some NPS (e.g., synthetic cannabinoids) showed more than 20% degradation. The method was successfully applied to the analysis of seven authentic oral fluid samples positive for 17 different analytes. The method achieved good sensitivity and simultaneous detection of a wide range of NPS. SN - 1945-2403 UR - https://www.unboundmedicine.com/medline/citation/32685960/Screening_of_104_New_Psychoactive_Substances__NPS__and_Other_Drugs_of_Abuse_in_Oral_Fluid_by_LC_MS_MS_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/bkaa089 DB - PRIME DP - Unbound Medicine ER -