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Screening and functional characterization of candidate resistance genes to powdery mildew from Dasypyrum villosum#4 in a wheat line Pm97033.
Theor Appl Genet. 2020 Nov; 133(11):3067-3083.TA

Abstract

KEY MESSAGE

Three genes designated DvLox, Pm21#4, and Pm21#4-H identified in a wheat-Dasypyrum villosum#4 T6V#4S·6DL translocation line Pm97033 conferred wheat for powdery mildew resistance. Powdery mildew (PM) caused by Blumeria graminis f. sp. tritici (Bgt) is one of the most devastating diseases in wheat. To date, only a few genes conferring resistance to wheat PM are cloned. Dasypyrum villosum is a wild relative of wheat, which provides Pm21 conferring wheat immunity to PM. In this study, we obtained many differentially expressed genes (DEGs) from a wheat-D. villosum#4 T6V#4S·6DL translocation line Pm97033 using RNA-sequencing. Among them, 7 DEGs associated with pathogen resistance were up-regulated in front of Bgt infection. Virus-induced gene silencing and transformation assays demonstrated that two of them, DvLox and Pm21#4 encoding a lipoxygenase and a encoding coiled-coil/nucleotide-binding site/leucine-rich repeat resistance protein, conferred wheat PM resistance. The transgenic wheat plants expressing DvLox enhanced PM resistance, and the transgenic wheat plants expressing Pm21#4 showed PM immunity. The Pm21#4-silenced Pm97033 plants by the cluster regularly interspaced short palindromic repeats-associated endonuclease (CRISPR/Cas9) system were susceptible to PM. Thus, Pm21#4 is a key gene contributing PM immune resistance in Pm97033. Constitutively expression of Pm21#4-H, which is silenced in Pm97033 and D. villosum#4, endowed a PM-susceptible wheat variety Fielder with PM immune resistance.

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Authors+Show Affiliations

Li S
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing, 100081, China.
Jia Z
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. Key Laboratory of Ministry of Agriculture and Rural Affairs of China for Biology and Genetic Breeding of Triticeae Crops, Beijing, 100081, China.
Wang K
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing, 100081, China.
Du L
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Li H
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Lin Z
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. linzhishan@caas.cn. National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing, 100081, China. linzhishan@caas.cn.
Ye X
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. yexingguo@caas.cn. Key Laboratory of Ministry of Agriculture and Rural Affairs of China for Biology and Genetic Breeding of Triticeae Crops, Beijing, 100081, China. yexingguo@caas.cn.

MeSH

AscomycotaCRISPR-Cas SystemsDisease ResistanceGenes, PlantLeucine-Rich Repeat ProteinsLipoxygenasePlant DiseasesPlant ImmunityPlants, Genetically ModifiedPoaceaeProteinsTranscriptomeTranslocation, GeneticTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32685983

Citation

Li, Shijin, et al. "Screening and Functional Characterization of Candidate Resistance Genes to Powdery Mildew From Dasypyrum Villosum#4 in a Wheat Line Pm97033." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 133, no. 11, 2020, pp. 3067-3083.
Li S, Jia Z, Wang K, et al. Screening and functional characterization of candidate resistance genes to powdery mildew from Dasypyrum villosum#4 in a wheat line Pm97033. Theor Appl Genet. 2020;133(11):3067-3083.
Li, S., Jia, Z., Wang, K., Du, L., Li, H., Lin, Z., & Ye, X. (2020). Screening and functional characterization of candidate resistance genes to powdery mildew from Dasypyrum villosum#4 in a wheat line Pm97033. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 133(11), 3067-3083. https://doi.org/10.1007/s00122-020-03655-4
Li S, et al. Screening and Functional Characterization of Candidate Resistance Genes to Powdery Mildew From Dasypyrum Villosum#4 in a Wheat Line Pm97033. Theor Appl Genet. 2020;133(11):3067-3083. PubMed PMID: 32685983.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Screening and functional characterization of candidate resistance genes to powdery mildew from Dasypyrum villosum#4 in a wheat line Pm97033. AU - Li,Shijin, AU - Jia,Zimiao, AU - Wang,Ke, AU - Du,Lipu, AU - Li,Hongjie, AU - Lin,Zhishan, AU - Ye,Xingguo, Y1 - 2020/07/19/ PY - 2019/11/05/received PY - 2020/07/08/accepted PY - 2020/7/21/pubmed PY - 2021/4/16/medline PY - 2020/7/21/entrez SP - 3067 EP - 3083 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 133 IS - 11 N2 - KEY MESSAGE: Three genes designated DvLox, Pm21#4, and Pm21#4-H identified in a wheat-Dasypyrum villosum#4 T6V#4S·6DL translocation line Pm97033 conferred wheat for powdery mildew resistance. Powdery mildew (PM) caused by Blumeria graminis f. sp. tritici (Bgt) is one of the most devastating diseases in wheat. To date, only a few genes conferring resistance to wheat PM are cloned. Dasypyrum villosum is a wild relative of wheat, which provides Pm21 conferring wheat immunity to PM. In this study, we obtained many differentially expressed genes (DEGs) from a wheat-D. villosum#4 T6V#4S·6DL translocation line Pm97033 using RNA-sequencing. Among them, 7 DEGs associated with pathogen resistance were up-regulated in front of Bgt infection. Virus-induced gene silencing and transformation assays demonstrated that two of them, DvLox and Pm21#4 encoding a lipoxygenase and a encoding coiled-coil/nucleotide-binding site/leucine-rich repeat resistance protein, conferred wheat PM resistance. The transgenic wheat plants expressing DvLox enhanced PM resistance, and the transgenic wheat plants expressing Pm21#4 showed PM immunity. The Pm21#4-silenced Pm97033 plants by the cluster regularly interspaced short palindromic repeats-associated endonuclease (CRISPR/Cas9) system were susceptible to PM. Thus, Pm21#4 is a key gene contributing PM immune resistance in Pm97033. Constitutively expression of Pm21#4-H, which is silenced in Pm97033 and D. villosum#4, endowed a PM-susceptible wheat variety Fielder with PM immune resistance. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/32685983/Screening_and_functional_characterization_of_candidate_resistance_genes_to_powdery_mildew_from_Dasypyrum_villosum DB - PRIME DP - Unbound Medicine ER -