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Danger in the Canopy. Comparative Proteomics and Bioactivities of the Venoms of the South American Palm Pit Viper Bothrops bilineatus Subspecies bilineatus and smaragdinus and Antivenomics of B. b. bilineatus (Rondônia) Venom against the Brazilian Pentabothropic Antivenom.
J Proteome Res. 2020 08 07; 19(8):3518-3532.JP

Abstract

We report a structural and functional proteomics characterization of venoms of the two subspecies (Bothrops bilineatus bilineatus and B. b. smaragdinus) of the South American palm pit viper from the Brazilian state of Rondônia and B. b. smaragdinus from Perú. These poorly known arboreal and mostly nocturnal generalist predators are widely distributed in lowland rainforests throughout the entire Amazon region, where they represent an important cause of snakebites. The three B. bilineatus spp. venom samples exhibit overall conserved proteomic profiles comprising components belonging to 11 venom protein classes, with PIII (34-40% of the total venom proteins) and PI (8-18%) SVMPs and their endogenous tripeptide inhibitors (SVMPi, 8-10%); bradykinin-potentiating-like peptides (BBPs, 10.7-15%); snake venom serine proteinases (SVSP, 5.5-14%); C-type lectin-like proteins (CTL, 3-10%); phospholipases A2 (PLA2, 2.8-7.6%); cysteine-rich secretory proteins (CRISP, 0.9-2.8%); l-amino acid oxidases (LAO, 0.9-5%) representing the major components of their common venom proteomes. Comparative analysis of the venom proteomes of the two geographic variants of B. b. smaragdinus with that of B. b. bilineatus revealed that the two Brazilian taxa share identical molecules between themselves but not with Peruvian B. b. smaragdinus, suggesting hybridization between the geographically close, possibly sympatric, Porto Velho (RO, BR) B. b. smaragdinus and B. b. bilineatus parental populations. However, limited sampling does not allow determining the frequency of this event. The toxin arsenal of the South American palm pit vipers may account for the in vitro recorded collagenolytic, caseinolytic, PLA2, l-amino acid oxidase, thrombin-like and factor X-activating activities, and the clinical features of South American palm pit viper envenomings, i.e., local and progressively ascending pain, shock and loss of consciousness, spontaneous bleeding, and profound coagulopathy. The remarkable cross-reactivity of the Brazilian pentabothropic SAB antivenom toward the heterologous B. b. bilineatus venom suggests that the paraspecific antigenic determinants should have been already present in the venom of the last common ancestor of the Bothrops ″jararaca″ and ″taeniatus″ clades, about 8.5 Mya in the mid-late Miocene epoch of the Cenozoic era. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the data set identifiers PXD020043, PXD020026, and PXD020013.

Authors+Show Affiliations

Laboratorio de Venómica Evolutiva y Traslacional, Consejo Superior de Investigaciones Científicas, Valencia 46010, Spain.Laboratorio de Venómica Evolutiva y Traslacional, Consejo Superior de Investigaciones Científicas, Valencia 46010, Spain.Laboratorio de Venómica Evolutiva y Traslacional, Consejo Superior de Investigaciones Científicas, Valencia 46010, Spain.Laboratório de Herpetologia, Instituto Butantan, São Paulo 05503-000, Brazil.Laboratório de Herpetologia, Instituto Butantan, São Paulo 05503-000, Brazil.Laboratório de Herpetologia, Instituto Butantan, São Paulo 05503-000, Brazil.Laboratório de Herpetologia, Instituto Butantan, São Paulo 05503-000, Brazil.Universidade Federal do Acre, Centro de Ciências Biológicas e da Natureza, Laboratório de Herpetologia, Rio Branco 69915-900, AC, Brazil.Centro de Estudos de Biomoléculas Aplicadas a Saúde (CEBio), Fundação Oswaldo Cruz, Fiocruz Rondônia, Porto Velho 76812-245, RO, Brazil.Departamento Académico de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima 31, Perú.Departamento Académico de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima 31, Perú.Centro de Estudos de Biomoléculas Aplicadas a Saúde (CEBio), Fundação Oswaldo Cruz, Fiocruz Rondônia, Porto Velho 76812-245, RO, Brazil. Programa de Pós-graduação em Biologia Experimental (PGBIOEXP), Universidade Federal de Rondônia (UNIR), Porto Velho 76801-974, Brazil. Centro Universitário São Lucas (UniSL), Porto Velho 76805-846, RO, Brazil. Instituto Nacional de Ciência e Tecnologia, Epidemiologia da Amazônia Ocidental (INCT-EpiAmO), Porto Velho 76812-245, RO, Brazil.Centro de Estudos de Biomoléculas Aplicadas a Saúde (CEBio), Fundação Oswaldo Cruz, Fiocruz Rondônia, Porto Velho 76812-245, RO, Brazil. Programa de Pós-graduação em Biologia Experimental (PGBIOEXP), Universidade Federal de Rondônia (UNIR), Porto Velho 76801-974, Brazil.Laboratório de Herpetologia, Instituto Butantan, São Paulo 05503-000, Brazil.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San Jose 11501, Costa Rica.Laboratorio de Venómica Evolutiva y Traslacional, Consejo Superior de Investigaciones Científicas, Valencia 46010, Spain.Centro de Estudos de Biomoléculas Aplicadas a Saúde (CEBio), Fundação Oswaldo Cruz, Fiocruz Rondônia, Porto Velho 76812-245, RO, Brazil. Programa de Pós-graduação em Biologia Experimental (PGBIOEXP), Universidade Federal de Rondônia (UNIR), Porto Velho 76801-974, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32686412

Citation

Sanz, Libia, et al. "Danger in the Canopy. Comparative Proteomics and Bioactivities of the Venoms of the South American Palm Pit Viper Bothrops Bilineatus Subspecies Bilineatus and Smaragdinus and Antivenomics of B. B. Bilineatus (Rondônia) Venom Against the Brazilian Pentabothropic Antivenom." Journal of Proteome Research, vol. 19, no. 8, 2020, pp. 3518-3532.
Sanz L, Quesada-Bernat S, Pérez A, et al. Danger in the Canopy. Comparative Proteomics and Bioactivities of the Venoms of the South American Palm Pit Viper Bothrops bilineatus Subspecies bilineatus and smaragdinus and Antivenomics of B. b. bilineatus (Rondônia) Venom against the Brazilian Pentabothropic Antivenom. J Proteome Res. 2020;19(8):3518-3532.
Sanz, L., Quesada-Bernat, S., Pérez, A., De Morais-Zani, K., SantˈAnna, S. S., Hatakeyama, D. M., Tasima, L. J., De Souza, M. B., Kayano, A. M., Zavaleta, A., Salas, M., Soares, A. M., Calderón, L. A., Tanaka-Azevedo, A. M., Lomonte, B., Calvete, J. J., & Caldeira, C. A. S. (2020). Danger in the Canopy. Comparative Proteomics and Bioactivities of the Venoms of the South American Palm Pit Viper Bothrops bilineatus Subspecies bilineatus and smaragdinus and Antivenomics of B. b. bilineatus (Rondônia) Venom against the Brazilian Pentabothropic Antivenom. Journal of Proteome Research, 19(8), 3518-3532. https://doi.org/10.1021/acs.jproteome.0c00337
Sanz L, et al. Danger in the Canopy. Comparative Proteomics and Bioactivities of the Venoms of the South American Palm Pit Viper Bothrops Bilineatus Subspecies Bilineatus and Smaragdinus and Antivenomics of B. B. Bilineatus (Rondônia) Venom Against the Brazilian Pentabothropic Antivenom. J Proteome Res. 2020 08 7;19(8):3518-3532. PubMed PMID: 32686412.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Danger in the Canopy. Comparative Proteomics and Bioactivities of the Venoms of the South American Palm Pit Viper Bothrops bilineatus Subspecies bilineatus and smaragdinus and Antivenomics of B. b. bilineatus (Rondônia) Venom against the Brazilian Pentabothropic Antivenom. AU - Sanz,Libia, AU - Quesada-Bernat,Sarai, AU - Pérez,Alicia, AU - De Morais-Zani,Karen, AU - SantˈAnna,Sávio S, AU - Hatakeyama,Daniela M, AU - Tasima,Lidia J, AU - De Souza,Moisés B, AU - Kayano,Anderson M, AU - Zavaleta,Alfonso, AU - Salas,Maria, AU - Soares,Andreimar M, AU - Calderón,Leonardo de A, AU - Tanaka-Azevedo,Anita M, AU - Lomonte,Bruno, AU - Calvete,Juan J, AU - Caldeira,Cleópatra A S, Y1 - 2020/07/30/ PY - 2020/7/21/pubmed PY - 2020/7/21/medline PY - 2020/7/21/entrez KW - Bothrops bilineatus KW - Brazilian pentabothropic antivenom KW - Comparative snake venomics KW - South American palm viper KW - antivenomics SP - 3518 EP - 3532 JF - Journal of proteome research JO - J Proteome Res VL - 19 IS - 8 N2 - We report a structural and functional proteomics characterization of venoms of the two subspecies (Bothrops bilineatus bilineatus and B. b. smaragdinus) of the South American palm pit viper from the Brazilian state of Rondônia and B. b. smaragdinus from Perú. These poorly known arboreal and mostly nocturnal generalist predators are widely distributed in lowland rainforests throughout the entire Amazon region, where they represent an important cause of snakebites. The three B. bilineatus spp. venom samples exhibit overall conserved proteomic profiles comprising components belonging to 11 venom protein classes, with PIII (34-40% of the total venom proteins) and PI (8-18%) SVMPs and their endogenous tripeptide inhibitors (SVMPi, 8-10%); bradykinin-potentiating-like peptides (BBPs, 10.7-15%); snake venom serine proteinases (SVSP, 5.5-14%); C-type lectin-like proteins (CTL, 3-10%); phospholipases A2 (PLA2, 2.8-7.6%); cysteine-rich secretory proteins (CRISP, 0.9-2.8%); l-amino acid oxidases (LAO, 0.9-5%) representing the major components of their common venom proteomes. Comparative analysis of the venom proteomes of the two geographic variants of B. b. smaragdinus with that of B. b. bilineatus revealed that the two Brazilian taxa share identical molecules between themselves but not with Peruvian B. b. smaragdinus, suggesting hybridization between the geographically close, possibly sympatric, Porto Velho (RO, BR) B. b. smaragdinus and B. b. bilineatus parental populations. However, limited sampling does not allow determining the frequency of this event. The toxin arsenal of the South American palm pit vipers may account for the in vitro recorded collagenolytic, caseinolytic, PLA2, l-amino acid oxidase, thrombin-like and factor X-activating activities, and the clinical features of South American palm pit viper envenomings, i.e., local and progressively ascending pain, shock and loss of consciousness, spontaneous bleeding, and profound coagulopathy. The remarkable cross-reactivity of the Brazilian pentabothropic SAB antivenom toward the heterologous B. b. bilineatus venom suggests that the paraspecific antigenic determinants should have been already present in the venom of the last common ancestor of the Bothrops ″jararaca″ and ″taeniatus″ clades, about 8.5 Mya in the mid-late Miocene epoch of the Cenozoic era. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the data set identifiers PXD020043, PXD020026, and PXD020013. SN - 1535-3907 UR - https://www.unboundmedicine.com/medline/citation/32686412/Danger_in_the_Canopy__Comparative_Proteomics_and_Bioactivities_of_the_Venoms_of_the_South_American_Palm_Pit_Viper_Bothrops_bilineatus_Subspecies_bilineatus_and_smaragdinus_and_Antivenomics_of_B__b__bilineatus__Rondônia__Venom_against_the_Brazilian_Pentabothropic_Antivenom_ L2 - https://doi.org/10.1021/acs.jproteome.0c00337 DB - PRIME DP - Unbound Medicine ER -