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Effectiveness of the guava leaf extracts against lipopolysaccharide-induced oxidative stress and immune responses in Cyprinus carpio.
Fish Shellfish Immunol. 2020 Oct; 105:164-176.FS

Abstract

The anti-inflammatory activity of the guava leaf extracts (GLE) against LPS-induced inflammatory responses in fish macrophage cell lines is well documented. Here, we evaluated the effects of dietary GLE on LPS-induced oxidative stress, immune responses, and glucocorticoid receptor-related gene expression in Cyprinus carpio. Basal diet was supplemented with 0 (control), 100, 150, 200, or 250 mg kg-1 GLE for eight weeks. Highest (p < 0.05) weight gain rate was obtained in fish group supplemented with 200 mg kg-1 of GLE. The results showed that superoxide dismutase, glutathione-S-transferase, glutathione peroxidase, glutathione reductase, lysozyme, and complement C3 decreased, while malondialdehyde level increased in the liver and spleen upon LPS-challenge. Dietary GLE supplementation (especially 200 or 250 mg kg-1) alleviated LPS-induced changes. Similarly, GLE (150-250 mg kg-1) reversed LPS-induced alteration of serum biochemical parameters such as alkaline phosphatase, aspartate transaminase, alanine transaminase, and myeloperoxidase. LPS treatment markedly induced increased the mRNA levels of TNF-α, IL-1β, and NF-κB p65 in both the liver and kidney tissues; however, GLE pre-treatment attenuated LPS-induced elicitation of TNF-α, IL-β, and NF-κB p65. Moreover, dietary GLE supplementation significantly increased the expression of HSP70 and HSP90, and glucocorticoid receptor in the liver and kidney after LPS challenge. Thus, GLE attenuated LPS-induced inflammation response by up-regulating glucocorticoid receptor-related gene expression in carp. Finally, GLE supplementation reduced carp mortality after LPS-challenge. These results suggest that dietary supplementation with 200 mg kg-1 GLE is adequate for effectively attenuating LPS-induced oxidative stress and immune-suppressive effects in C. carpio.

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Authors+Show Affiliations

Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, South Korea.

Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, South Korea.

Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, South Korea.

Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, South Korea.

Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, South Korea.

Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, South Korea.

Dept. of Zoology, Kundavai Nachiyar Government Arts College for Women (Autonomous), Thanjavur, 613007, Tamil Nadu, India. Electronic address: drvsukumar@gmail.com.

Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, South Korea. Electronic address: parksec@snu.ac.kr.

MeSH

Adaptive ImmunityAnimalsCarpsImmunity, InnateLipopolysaccharidesOxidative StressPlant ExtractsPlant LeavesPsidium

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32687879

Citation

Giri, Sib Sankar, et al. "Effectiveness of the Guava Leaf Extracts Against Lipopolysaccharide-induced Oxidative Stress and Immune Responses in Cyprinus Carpio." Fish & Shellfish Immunology, vol. 105, 2020, pp. 164-176.

Giri SS, Kim HJ, Kim SG, et al. Effectiveness of the guava leaf extracts against lipopolysaccharide-induced oxidative stress and immune responses in Cyprinus carpio. Fish Shellfish Immunol. 2020;105:164-176.

Giri, S. S., Kim, H. J., Kim, S. G., Kim, S. W., Kwon, J., Lee, S. B., Sukumaran, V., & Chang Park, S. (2020). Effectiveness of the guava leaf extracts against lipopolysaccharide-induced oxidative stress and immune responses in Cyprinus carpio. Fish & Shellfish Immunology, 105, 164-176. https://doi.org/10.1016/j.fsi.2020.06.004

Giri SS, et al. Effectiveness of the Guava Leaf Extracts Against Lipopolysaccharide-induced Oxidative Stress and Immune Responses in Cyprinus Carpio. Fish Shellfish Immunol. 2020;105:164-176. PubMed PMID: 32687879.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Effectiveness of the guava leaf extracts against lipopolysaccharide-induced oxidative stress and immune responses in Cyprinus carpio. AU - Giri,Sib Sankar, AU - Kim,Hyoun Joong, AU - Kim,Sang Guen, AU - Kim,Sang Wha, AU - Kwon,Jun, AU - Lee,Sung Bin, AU - Sukumaran,V, AU - Chang Park,Se, Y1 - 2020/07/18/ PY - 2020/02/13/received PY - 2020/05/30/revised PY - 2020/06/02/accepted PY - 2020/7/21/pubmed PY - 2021/4/17/medline PY - 2020/7/21/entrez KW - Glucocorticoid receptor KW - Guava leaf extract KW - HSPs KW - Kidney KW - Oxidative stress KW - Serum SP - 164 EP - 176 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 105 N2 - The anti-inflammatory activity of the guava leaf extracts (GLE) against LPS-induced inflammatory responses in fish macrophage cell lines is well documented. Here, we evaluated the effects of dietary GLE on LPS-induced oxidative stress, immune responses, and glucocorticoid receptor-related gene expression in Cyprinus carpio. Basal diet was supplemented with 0 (control), 100, 150, 200, or 250 mg kg-1 GLE for eight weeks. Highest (p < 0.05) weight gain rate was obtained in fish group supplemented with 200 mg kg-1 of GLE. The results showed that superoxide dismutase, glutathione-S-transferase, glutathione peroxidase, glutathione reductase, lysozyme, and complement C3 decreased, while malondialdehyde level increased in the liver and spleen upon LPS-challenge. Dietary GLE supplementation (especially 200 or 250 mg kg-1) alleviated LPS-induced changes. Similarly, GLE (150-250 mg kg-1) reversed LPS-induced alteration of serum biochemical parameters such as alkaline phosphatase, aspartate transaminase, alanine transaminase, and myeloperoxidase. LPS treatment markedly induced increased the mRNA levels of TNF-α, IL-1β, and NF-κB p65 in both the liver and kidney tissues; however, GLE pre-treatment attenuated LPS-induced elicitation of TNF-α, IL-β, and NF-κB p65. Moreover, dietary GLE supplementation significantly increased the expression of HSP70 and HSP90, and glucocorticoid receptor in the liver and kidney after LPS challenge. Thus, GLE attenuated LPS-induced inflammation response by up-regulating glucocorticoid receptor-related gene expression in carp. Finally, GLE supplementation reduced carp mortality after LPS-challenge. These results suggest that dietary supplementation with 200 mg kg-1 GLE is adequate for effectively attenuating LPS-induced oxidative stress and immune-suppressive effects in C. carpio. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/32687879/Effectiveness_of_the_guava_leaf_extracts_against_lipopolysaccharide_induced_oxidative_stress_and_immune_responses_in_Cyprinus_carpio_ DB - PRIME DP - Unbound Medicine ER -