Tags

Type your tag names separated by a space and hit enter

[Effect of electroacupuncture on intestinal Toll-like receptor 4 and nuclear factor-kappa B in obese rats].
Zhen Ci Yan Jiu. 2020 Jul 25; 45(7):541-7.ZC

Abstract

OBJECTIVE

To investigate the effect of electroacupuncture (EA) on intestinal Toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NF-κB) in obese rats, so as to explore the mechanism of action of acupuncture in losing weight.

METHODS

A total of 50 male Wistar rats were randomly divided into control and model groups. High-fat feed was used to establish a rat model of obesity, and after modeling, the 24 rats were randomly divided into model group, TLR4 inhibitor group, and EA group, with 8 rats in each group. The rats in the EA group were given EA at "Guanyuan" (CV4), "Zhongwan "(CV12), "Zusanli" (ST36), and" Fenglong" (ST40), 10 minutes each time, 3 times a week, and those in the TLR4 inhibitor group were given intraperitoneal injection of TAK-242 three times a week; the course of treatment was 8 weeks for both groups. Body weight and blood glucose were measured every two weeks. Co-immunoprecipitation was used to observe the interaction between TLR4 and NF-κB p65 in the intestinal tissue; electrophoretic mobility shift assay was used to measure the activity of NF-κB p65; Western blot was used to measure the protein expression of TLR4, phosphorylated nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (p-IκBα), and NF-κB p65; quantitative real-time PCR was used to measure the mRNA expression of TLR4, NF-κB p65, and IκBα.

RESULTS

Compared with the control group, the model group had significant increases in body weight, blood glucose, and protein and mRNA expression of TLR4 and NF-κB p65 (P<0.01, P<0.05), as well as significant enhancement in the interaction between TLR4 and NF-κB p65 and activity of NF-κB p65 (P<0.05,P<0.01). Compared with the model group, the EA group had a significant reduction in body weight (P<0.05), both of the EA group and the TLR4 inhibitor group had significant reductions in blood glucose, and protein and mRNA expression of TLR4, p-IκBα, and NF-κB p65 (P<0.05,P<0.01), as well as significant reductions in the activity of NF-κB p65 (P<0.01).

CONCLUSION

EA can effectively regulate intestinal TLR4, inhibit the interaction between TLR4 and NF-κB p65, and reduce the activity of NF-κB p65, which may be a potential mechanism of EA in reducing body weight and blood glucose in obese rats.

Authors+Show Affiliations

College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, Wuhan 430061, China.College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, Wuhan 430061, China.College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, Wuhan 430061, China.School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430061, China.College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, Wuhan 430061, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

32705827

Citation

Chen, Li, et al. "[Effect of Electroacupuncture On Intestinal Toll-like Receptor 4 and Nuclear Factor-kappa B in Obese Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 45, no. 7, 2020, pp. 541-7.
Chen L, Wang JZ, Zhou GW, et al. [Effect of electroacupuncture on intestinal Toll-like receptor 4 and nuclear factor-kappa B in obese rats]. Zhen Ci Yan Jiu. 2020;45(7):541-7.
Chen, L., Wang, J. Z., Zhou, G. W., Wu, Y. G., & Liang, F. X. (2020). [Effect of electroacupuncture on intestinal Toll-like receptor 4 and nuclear factor-kappa B in obese rats]. Zhen Ci Yan Jiu = Acupuncture Research, 45(7), 541-7. https://doi.org/10.13702/j.1000-0607.190736
Chen L, et al. [Effect of Electroacupuncture On Intestinal Toll-like Receptor 4 and Nuclear Factor-kappa B in Obese Rats]. Zhen Ci Yan Jiu. 2020 Jul 25;45(7):541-7. PubMed PMID: 32705827.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture on intestinal Toll-like receptor 4 and nuclear factor-kappa B in obese rats]. AU - Chen,Li, AU - Wang,Jing-Zhi, AU - Zhou,Guang-Wen, AU - Wu,Yong-Gui, AU - Liang,Feng-Xia, PY - 2020/7/25/entrez PY - 2020/7/25/pubmed PY - 2020/9/17/medline KW - Electroacupuncture KW - Intestinal tract KW - Nuclear factor-kappa B KW - Obesity KW - Toll-like receptor 4 SP - 541 EP - 7 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 45 IS - 7 N2 - OBJECTIVE: To investigate the effect of electroacupuncture (EA) on intestinal Toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NF-κB) in obese rats, so as to explore the mechanism of action of acupuncture in losing weight. METHODS: A total of 50 male Wistar rats were randomly divided into control and model groups. High-fat feed was used to establish a rat model of obesity, and after modeling, the 24 rats were randomly divided into model group, TLR4 inhibitor group, and EA group, with 8 rats in each group. The rats in the EA group were given EA at "Guanyuan" (CV4), "Zhongwan "(CV12), "Zusanli" (ST36), and" Fenglong" (ST40), 10 minutes each time, 3 times a week, and those in the TLR4 inhibitor group were given intraperitoneal injection of TAK-242 three times a week; the course of treatment was 8 weeks for both groups. Body weight and blood glucose were measured every two weeks. Co-immunoprecipitation was used to observe the interaction between TLR4 and NF-κB p65 in the intestinal tissue; electrophoretic mobility shift assay was used to measure the activity of NF-κB p65; Western blot was used to measure the protein expression of TLR4, phosphorylated nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (p-IκBα), and NF-κB p65; quantitative real-time PCR was used to measure the mRNA expression of TLR4, NF-κB p65, and IκBα. RESULTS: Compared with the control group, the model group had significant increases in body weight, blood glucose, and protein and mRNA expression of TLR4 and NF-κB p65 (P<0.01, P<0.05), as well as significant enhancement in the interaction between TLR4 and NF-κB p65 and activity of NF-κB p65 (P<0.05,P<0.01). Compared with the model group, the EA group had a significant reduction in body weight (P<0.05), both of the EA group and the TLR4 inhibitor group had significant reductions in blood glucose, and protein and mRNA expression of TLR4, p-IκBα, and NF-κB p65 (P<0.05,P<0.01), as well as significant reductions in the activity of NF-κB p65 (P<0.01). CONCLUSION: EA can effectively regulate intestinal TLR4, inhibit the interaction between TLR4 and NF-κB p65, and reduce the activity of NF-κB p65, which may be a potential mechanism of EA in reducing body weight and blood glucose in obese rats. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/32705827/[Effect_of_electroacupuncture_on_intestinal_Toll_like_receptor_4_and_nuclear_factor_kappa_B_in_obese_rats]_ DB - PRIME DP - Unbound Medicine ER -