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[Effect of acupoint catgut embedding on levels of PG-related factors and NF-κB proteins in ute-rine tissues of rats with primary dysmenorrhea].
Zhen Ci Yan Jiu. 2020 Jul 25; 45(7):548-51.ZC

Abstract

OBJECTIVE

To observe the effect of acupoint catgut embedding on levels of prostaglandin F2α(PGF2α), cyclooxygenase-2(COX-2) and nuclear factor kappa B(NF-κB) in the uteruses of rats with primary dysmenorrhea (PD), so as to explore its mechanisms underlying improvement of PD.

METHODS

Forty female SD rats were randomly divided into normal, model, acupoint catgut embedment and medication groups, with 10 rats in each group. The PD model was established by subcutaneous injection of estradiol benzoate (0.5 mg on the 1st day, and 0.2 mg thereafter) once daily for 10 days, followed by i.p. of oxytocin 2 U(0.5 mg•5 U-1•mL-1) on the 11th day. Catgut embedment was applied to "Guanyuan"(CV4) and "Sanyinjiao"(SP6) on day 1 and 5 while modeling, and rats of the medication group received gavage of ibuprofen (1.25 mg/mL, 0.8 mL/rat) once daily for 10 d. The level of PGF2αin the uterus tissuewas assayed by ELISA, and the expression levels of uterine COX-2, phospho (p)-NF-κB p65, NF-κB p65 proteins were detected by Western blot.

RESULTS

Compared with the normal group, the levels of PGF2α, COX-2, p-NF-κB p65 and NF-κB p65 in the uterine tissues were significantly increased in the model group (P<0.05). Compared with the model group, the levels of PGF2α and COX-2 in both catgut embedment and medication groups as well as p-NF-κB p65 in the catgut embedment group were significantly decreased (P<0.05). Compared with the medication group, the level of p-NF-κB 65 in the catgut embedment group was significantly decreased (P<0.05).

CONCLUSION

The analgesic effect of acupoint catgut embedding may be related to its effects in down-regulating the expression of p-NF-κB and the levels of COX-2 and PGF2αin uteruses of PD rats.

Authors+Show Affiliations

Medical School, Hunan University of Chinese Medicine, Changsha 410208, China.Medical School, Hunan University of Chinese Medicine, Changsha 410208, China.Medical School, Hunan University of Chinese Medicine, Changsha 410208, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

32705828

Citation

Tang, Wen-Jing, et al. "[Effect of Acupoint Catgut Embedding On Levels of PG-related Factors and NF-κB Proteins in Ute-rine Tissues of Rats With Primary Dysmenorrhea]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 45, no. 7, 2020, pp. 548-51.
Tang WJ, Wang YQ, Tang B. [Effect of acupoint catgut embedding on levels of PG-related factors and NF-κB proteins in ute-rine tissues of rats with primary dysmenorrhea]. Zhen Ci Yan Jiu. 2020;45(7):548-51.
Tang, W. J., Wang, Y. Q., & Tang, B. (2020). [Effect of acupoint catgut embedding on levels of PG-related factors and NF-κB proteins in ute-rine tissues of rats with primary dysmenorrhea]. Zhen Ci Yan Jiu = Acupuncture Research, 45(7), 548-51. https://doi.org/10.13702/j.1000-0607.190687
Tang WJ, Wang YQ, Tang B. [Effect of Acupoint Catgut Embedding On Levels of PG-related Factors and NF-κB Proteins in Ute-rine Tissues of Rats With Primary Dysmenorrhea]. Zhen Ci Yan Jiu. 2020 Jul 25;45(7):548-51. PubMed PMID: 32705828.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of acupoint catgut embedding on levels of PG-related factors and NF-κB proteins in ute-rine tissues of rats with primary dysmenorrhea]. AU - Tang,Wen-Jing, AU - Wang,Yi-Qin, AU - Tang,Biao, PY - 2020/7/25/entrez PY - 2020/7/25/pubmed PY - 2020/9/17/medline KW - Cyclooxygenase-2 KW - Acupoint catgut embedding KW - Nuclear factor kappa B KW - Primary dysmenorrhea KW - Prostaglandin F2α KW - Uterine SP - 548 EP - 51 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 45 IS - 7 N2 - OBJECTIVE: To observe the effect of acupoint catgut embedding on levels of prostaglandin F2α(PGF2α), cyclooxygenase-2(COX-2) and nuclear factor kappa B(NF-κB) in the uteruses of rats with primary dysmenorrhea (PD), so as to explore its mechanisms underlying improvement of PD. METHODS: Forty female SD rats were randomly divided into normal, model, acupoint catgut embedment and medication groups, with 10 rats in each group. The PD model was established by subcutaneous injection of estradiol benzoate (0.5 mg on the 1st day, and 0.2 mg thereafter) once daily for 10 days, followed by i.p. of oxytocin 2 U(0.5 mg•5 U-1•mL-1) on the 11th day. Catgut embedment was applied to "Guanyuan"(CV4) and "Sanyinjiao"(SP6) on day 1 and 5 while modeling, and rats of the medication group received gavage of ibuprofen (1.25 mg/mL, 0.8 mL/rat) once daily for 10 d. The level of PGF2αin the uterus tissuewas assayed by ELISA, and the expression levels of uterine COX-2, phospho (p)-NF-κB p65, NF-κB p65 proteins were detected by Western blot. RESULTS: Compared with the normal group, the levels of PGF2α, COX-2, p-NF-κB p65 and NF-κB p65 in the uterine tissues were significantly increased in the model group (P<0.05). Compared with the model group, the levels of PGF2α and COX-2 in both catgut embedment and medication groups as well as p-NF-κB p65 in the catgut embedment group were significantly decreased (P<0.05). Compared with the medication group, the level of p-NF-κB 65 in the catgut embedment group was significantly decreased (P<0.05). CONCLUSION: The analgesic effect of acupoint catgut embedding may be related to its effects in down-regulating the expression of p-NF-κB and the levels of COX-2 and PGF2αin uteruses of PD rats. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/32705828/[Effect_of_acupoint_catgut_embedding_on_levels_of_PG_related_factors_and_NF_κB_proteins_in_ute_rine_tissues_of_rats_with_primary_dysmenorrhea]_ L2 - https://antibodies.cancer.gov/detail/CPTC-PTGS2-1 DB - PRIME DP - Unbound Medicine ER -