Tags

Type your tag names separated by a space and hit enter

Effects of Adult Müller Cells and Their Conditioned Media on the Survival of Stem Cell-Derived Retinal Ganglion Cells.
Cells. 2020 07 22; 9(8)C

Abstract

Retinal neurons, particularly retinal ganglion cells (RGCs), are susceptible to the degenerative damage caused by different inherited conditions and environmental insults, leading to irreversible vision loss and, ultimately, blindness. Numerous strategies are being tested in different models of degeneration to restore vision and, in recent years, stem cell technologies have offered novel avenues to obtain donor cells for replacement therapies. To date, stem cell-based transplantation in the retina has been attempted as treatment for photoreceptor degeneration, but the same tools could potentially be applied to other retinal cell types, including RGCs. However, RGC-like cells are not an abundant cell type in stem cell-derived cultures and, often, these cells degenerate over time in vitro. To overcome this limitation, we have taken advantage of the neuroprotective properties of Müller glia (one of the main glial cell types in the retina) and we have examined whether Müller glia and the factors they secrete could promote RGC-like cell survival in organoid cultures. Accordingly, stem cell-derived RGC-like cells were co-cultured with adult Müller cells or Müller cell-conditioned media was added to the cultures. Remarkably, RGC-like cell survival was substantially enhanced in both culture conditions, and we also observed a significant increase in their neurite length. Interestingly, Atoh7, a transcription factor required for RGC development, was up-regulated in stem cell-derived organoids exposed to conditioned media, suggesting that Müller cells may also enhance the survival of retinal progenitors and/or postmitotic precursor cells. In conclusion, Müller cells and the factors they release promote organoid-derived RGC-like cell survival, neuritogenesis, and possibly neuronal maturation.

Authors+Show Affiliations

Department of Cell Biology and Histology, University of Basque Country UPV/EHU, Leioa, 48940 Vizcaya, Spain.Department of Cell Biology and Human Anatomy, University of California Davis, Davis, CA 95616, USA.Department of Cell Biology and Human Anatomy, University of California Davis, Davis, CA 95616, USA.Department of Cell Biology and Histology, University of Basque Country UPV/EHU, Leioa, 48940 Vizcaya, Spain.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32708020

Citation

Pereiro, Xandra, et al. "Effects of Adult Müller Cells and Their Conditioned Media On the Survival of Stem Cell-Derived Retinal Ganglion Cells." Cells, vol. 9, no. 8, 2020.
Pereiro X, Miltner AM, La Torre A, et al. Effects of Adult Müller Cells and Their Conditioned Media on the Survival of Stem Cell-Derived Retinal Ganglion Cells. Cells. 2020;9(8).
Pereiro, X., Miltner, A. M., La Torre, A., & Vecino, E. (2020). Effects of Adult Müller Cells and Their Conditioned Media on the Survival of Stem Cell-Derived Retinal Ganglion Cells. Cells, 9(8). https://doi.org/10.3390/cells9081759
Pereiro X, et al. Effects of Adult Müller Cells and Their Conditioned Media On the Survival of Stem Cell-Derived Retinal Ganglion Cells. Cells. 2020 07 22;9(8) PubMed PMID: 32708020.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Adult Müller Cells and Their Conditioned Media on the Survival of Stem Cell-Derived Retinal Ganglion Cells. AU - Pereiro,Xandra, AU - Miltner,Adam M, AU - La Torre,Anna, AU - Vecino,Elena, Y1 - 2020/07/22/ PY - 2020/06/05/received PY - 2020/07/13/revised PY - 2020/07/15/accepted PY - 2020/7/26/entrez PY - 2020/7/28/pubmed PY - 2021/3/11/medline KW - Müller glia KW - Stem cells KW - neuritogenesis KW - neuroprotection KW - retinal ganglion cells KW - retinal organoids JF - Cells JO - Cells VL - 9 IS - 8 N2 - Retinal neurons, particularly retinal ganglion cells (RGCs), are susceptible to the degenerative damage caused by different inherited conditions and environmental insults, leading to irreversible vision loss and, ultimately, blindness. Numerous strategies are being tested in different models of degeneration to restore vision and, in recent years, stem cell technologies have offered novel avenues to obtain donor cells for replacement therapies. To date, stem cell-based transplantation in the retina has been attempted as treatment for photoreceptor degeneration, but the same tools could potentially be applied to other retinal cell types, including RGCs. However, RGC-like cells are not an abundant cell type in stem cell-derived cultures and, often, these cells degenerate over time in vitro. To overcome this limitation, we have taken advantage of the neuroprotective properties of Müller glia (one of the main glial cell types in the retina) and we have examined whether Müller glia and the factors they secrete could promote RGC-like cell survival in organoid cultures. Accordingly, stem cell-derived RGC-like cells were co-cultured with adult Müller cells or Müller cell-conditioned media was added to the cultures. Remarkably, RGC-like cell survival was substantially enhanced in both culture conditions, and we also observed a significant increase in their neurite length. Interestingly, Atoh7, a transcription factor required for RGC development, was up-regulated in stem cell-derived organoids exposed to conditioned media, suggesting that Müller cells may also enhance the survival of retinal progenitors and/or postmitotic precursor cells. In conclusion, Müller cells and the factors they release promote organoid-derived RGC-like cell survival, neuritogenesis, and possibly neuronal maturation. SN - 2073-4409 UR - https://www.unboundmedicine.com/medline/citation/32708020/Effects_of_Adult_Müller_Cells_and_Their_Conditioned_Media_on_the_Survival_of_Stem_Cell_Derived_Retinal_Ganglion_Cells_ L2 - https://www.mdpi.com/resolver?pii=cells9081759 DB - PRIME DP - Unbound Medicine ER -