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The HD-ZIP IV transcription factor Tril regulates fruit spine density through gene dosage effects in cucumber.
J Exp Bot. 2020 10 22; 71(20):6297-6310.JE

Abstract

Trichomes and fruit spines are important traits that directly affect the appearance quality and commercial value of cucumber (Cucumis sativus). Tril (Trichome-less), encodes a HD-Zip IV transcription factor that plays a crucial role in the initiation of trichomes and fruit spines, but little is known about the details of the regulatory mechanisms involved. In this study, analysis of tissue expression patterns indicated that Tril is expressed and functions in the early stages of organ initiation and development. Expression of Tril under the control of its own promoter (the TrilPro::Tril-3*flag fragment) could partly rescue the mutant phenotypes of tril, csgl3 (cucumber glabrous 3, an allelic mutant of tril), and fs1 (few spines 1, a fragment substitution in the Tril promoter region), providing further evidence that Tril is responsible for the initiation of trichomes and fruit spines. In lines with dense spine, fs1-type lines, and transgenic lines of different backgrounds containing the TrilPro::Tril-3*flag foreign fragment, spine density increased in conjunction with increases in Tril expression, indicating that Tril has a gene dosage effect on fruit spine density in cucumber. Numerous Spines (NS) is a negative regulatory factor of fruit spine density. Characterization of the molecular and genetic interaction between Tril and NS/ns demonstrated that Tril functions upstream of NS with respect to spine initiation. Overall, our results reveal a novel regulatory mechanism governing the effect of Tril on fruit spine development, and provide a reference for future work on breeding for physical quality in cucumber.

Authors+Show Affiliations

School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China. State Key Laboratory of Vegetable Germplasm Innovation, Tianjin, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32710537

Citation

Du, Hui, et al. "The HD-ZIP IV Transcription Factor Tril Regulates Fruit Spine Density Through Gene Dosage Effects in Cucumber." Journal of Experimental Botany, vol. 71, no. 20, 2020, pp. 6297-6310.
Du H, Wang G, Pan J, et al. The HD-ZIP IV transcription factor Tril regulates fruit spine density through gene dosage effects in cucumber. J Exp Bot. 2020;71(20):6297-6310.
Du, H., Wang, G., Pan, J., Chen, Y., Xiao, T., Zhang, L., Zhang, K., Wen, H., Xiong, L., Yu, Y., He, H., Pan, J., & Cai, R. (2020). The HD-ZIP IV transcription factor Tril regulates fruit spine density through gene dosage effects in cucumber. Journal of Experimental Botany, 71(20), 6297-6310. https://doi.org/10.1093/jxb/eraa344
Du H, et al. The HD-ZIP IV Transcription Factor Tril Regulates Fruit Spine Density Through Gene Dosage Effects in Cucumber. J Exp Bot. 2020 10 22;71(20):6297-6310. PubMed PMID: 32710537.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The HD-ZIP IV transcription factor Tril regulates fruit spine density through gene dosage effects in cucumber. AU - Du,Hui, AU - Wang,Gang, AU - Pan,Jian, AU - Chen,Yue, AU - Xiao,Tingting, AU - Zhang,Leyu, AU - Zhang,Keyan, AU - Wen,Haifan, AU - Xiong,Liangrong, AU - Yu,Yao, AU - He,Huanle, AU - Pan,Junsong, AU - Cai,Run, PY - 2020/04/13/received PY - 2020/07/21/accepted PY - 2020/7/28/pubmed PY - 2021/5/15/medline PY - 2020/7/26/entrez KW - Cucumis sativus KW - Numerous Spines KW - Trichome-less KW - Tril KW - ns KW - Cucumber KW - HD-Zip IV KW - fruit spines KW - trichomes SP - 6297 EP - 6310 JF - Journal of experimental botany JO - J Exp Bot VL - 71 IS - 20 N2 - Trichomes and fruit spines are important traits that directly affect the appearance quality and commercial value of cucumber (Cucumis sativus). Tril (Trichome-less), encodes a HD-Zip IV transcription factor that plays a crucial role in the initiation of trichomes and fruit spines, but little is known about the details of the regulatory mechanisms involved. In this study, analysis of tissue expression patterns indicated that Tril is expressed and functions in the early stages of organ initiation and development. Expression of Tril under the control of its own promoter (the TrilPro::Tril-3*flag fragment) could partly rescue the mutant phenotypes of tril, csgl3 (cucumber glabrous 3, an allelic mutant of tril), and fs1 (few spines 1, a fragment substitution in the Tril promoter region), providing further evidence that Tril is responsible for the initiation of trichomes and fruit spines. In lines with dense spine, fs1-type lines, and transgenic lines of different backgrounds containing the TrilPro::Tril-3*flag foreign fragment, spine density increased in conjunction with increases in Tril expression, indicating that Tril has a gene dosage effect on fruit spine density in cucumber. Numerous Spines (NS) is a negative regulatory factor of fruit spine density. Characterization of the molecular and genetic interaction between Tril and NS/ns demonstrated that Tril functions upstream of NS with respect to spine initiation. Overall, our results reveal a novel regulatory mechanism governing the effect of Tril on fruit spine development, and provide a reference for future work on breeding for physical quality in cucumber. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/32710537/The_HD_ZIP_IV_transcription_factor_Tril_regulates_fruit_spine_density_through_gene_dosage_effects_in_cucumber_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/eraa344 DB - PRIME DP - Unbound Medicine ER -