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Synthesis and characterization of proanthocyanidin-chitosan nanoparticles: An assessment on human colorectal carcinoma HT-29 cells.
J Photochem Photobiol B. 2020 Sep; 210:111966.JP

Abstract

Cancer nanotheranostic materials are helpful in monitoring drug delivery and efficacy against tumor cells. Current chemotherapeutic may have adverse side effects and this necessity to discover the new modern therapeutic nano-drugs. In the present study, we designed the new targeted and degradable polymer of bio-active chitosan nanoparticles with proanthocyanidin (PAC-CSNPs) and evaluated its apoptotic effects against human colorectal carcinoma cells (HT-29). The functional groups were characterized by Fourier-transform infrared spectroscopy and transmission electron microscope. Further, their dispersion of spherical form nanoparticle with an average size of 73.43 nm used for drug delivery system. The PAC-CSNPs were targeted to inhibit the cyclin-dependent kinases and prevent cell cycle/cell division in cancer cells. At high concentrations of PAC (25 μg/mL) exposure, cell viability of HT-29 cells was greater than 80%. However, at low concentrations of PAC-CSNPs (6.25 μg/mL) exposure, HT-29 cell mortality was high, which may be due to the efficient drug release by CSNPs. The percentage of reactive oxygen species (ROS) levels were 12 ± 2.52% (control), 39 ± 4.32% (PAC), and 85.06 ± 3.54% (PAC-CSNPs). The over production of ROS by PAC-CSNPs can prompt DNA damage, cell death and apoptosis in HT-29 cells. The in vivo toxicity of synthesized PAC-CSNPs was tested against zebra fish observed at dose-time-dependent intervals. In conclusion, the PAC-CSNPs enhanced HT-29 cell death and shows promise as a novel future nano-therapy for cancer.

Authors+Show Affiliations

Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode 637 215, Tamil Nadu, India.Department of Food Science and Biotechnology, College of Life Sciences, Sejong University, Seoul 05006, South Korea. Electronic address: bala.m.k@sejong.ac.kr.Department of Environmental Energy and Engineering, Kyonggi University, Youngtong-Gu, Suwon, Gyeonggi-Do 16227, South Korea.Department of Environmental Energy and Engineering, Kyonggi University, Youngtong-Gu, Suwon, Gyeonggi-Do 16227, South Korea.Department of Botany, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India. Electronic address: cancerbiology41@gmail.com.Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia; Entomology Research Institute, Loyola College, Chennai 600034, Tamilnadu, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32711334

Citation

Mani, Suganya, et al. "Synthesis and Characterization of Proanthocyanidin-chitosan Nanoparticles: an Assessment On Human Colorectal Carcinoma HT-29 Cells." Journal of Photochemistry and Photobiology. B, Biology, vol. 210, 2020, p. 111966.
Mani S, Balasubramanian B, Balasubramani R, et al. Synthesis and characterization of proanthocyanidin-chitosan nanoparticles: An assessment on human colorectal carcinoma HT-29 cells. J Photochem Photobiol B, Biol. 2020;210:111966.
Mani, S., Balasubramanian, B., Balasubramani, R., Chang, S. W., Ponnusamy, P., Esmail, G. A., Arasu, M. V., Al-Dhabi, N. A., & Duraipandiyan, V. (2020). Synthesis and characterization of proanthocyanidin-chitosan nanoparticles: An assessment on human colorectal carcinoma HT-29 cells. Journal of Photochemistry and Photobiology. B, Biology, 210, 111966. https://doi.org/10.1016/j.jphotobiol.2020.111966
Mani S, et al. Synthesis and Characterization of Proanthocyanidin-chitosan Nanoparticles: an Assessment On Human Colorectal Carcinoma HT-29 Cells. J Photochem Photobiol B, Biol. 2020;210:111966. PubMed PMID: 32711334.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis and characterization of proanthocyanidin-chitosan nanoparticles: An assessment on human colorectal carcinoma HT-29 cells. AU - Mani,Suganya, AU - Balasubramanian,Balamuralikrishnan, AU - Balasubramani,Ravindran, AU - Chang,Soon Woong, AU - Ponnusamy,Ponmurugan, AU - Esmail,Galal Ali, AU - Arasu,Mariadhas Valan, AU - Al-Dhabi,Naif Abdullah, AU - Duraipandiyan,Veeramuthu, Y1 - 2020/07/17/ PY - 2020/01/26/received PY - 2020/07/06/revised PY - 2020/07/15/accepted PY - 2020/7/28/pubmed PY - 2020/7/28/medline PY - 2020/7/26/entrez KW - Chitosan nanoparticle KW - Colorectal cancer KW - Proanthocyanidin KW - in vitro and in vivo SP - 111966 EP - 111966 JF - Journal of photochemistry and photobiology. B, Biology JO - J. Photochem. Photobiol. B, Biol. VL - 210 N2 - Cancer nanotheranostic materials are helpful in monitoring drug delivery and efficacy against tumor cells. Current chemotherapeutic may have adverse side effects and this necessity to discover the new modern therapeutic nano-drugs. In the present study, we designed the new targeted and degradable polymer of bio-active chitosan nanoparticles with proanthocyanidin (PAC-CSNPs) and evaluated its apoptotic effects against human colorectal carcinoma cells (HT-29). The functional groups were characterized by Fourier-transform infrared spectroscopy and transmission electron microscope. Further, their dispersion of spherical form nanoparticle with an average size of 73.43 nm used for drug delivery system. The PAC-CSNPs were targeted to inhibit the cyclin-dependent kinases and prevent cell cycle/cell division in cancer cells. At high concentrations of PAC (25 μg/mL) exposure, cell viability of HT-29 cells was greater than 80%. However, at low concentrations of PAC-CSNPs (6.25 μg/mL) exposure, HT-29 cell mortality was high, which may be due to the efficient drug release by CSNPs. The percentage of reactive oxygen species (ROS) levels were 12 ± 2.52% (control), 39 ± 4.32% (PAC), and 85.06 ± 3.54% (PAC-CSNPs). The over production of ROS by PAC-CSNPs can prompt DNA damage, cell death and apoptosis in HT-29 cells. The in vivo toxicity of synthesized PAC-CSNPs was tested against zebra fish observed at dose-time-dependent intervals. In conclusion, the PAC-CSNPs enhanced HT-29 cell death and shows promise as a novel future nano-therapy for cancer. SN - 1873-2682 UR - https://www.unboundmedicine.com/medline/citation/32711334/Synthesis_and_characterization_of_proanthocyanidin_chitosan_nanoparticles:_An_assessment_on_human_colorectal_carcinoma_HT_29_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1011-1344(20)30416-4 DB - PRIME DP - Unbound Medicine ER -
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