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Extraction and structural analysis of Angelica sinensis polysaccharide with low molecular weight and its lipid-lowering effect on nonalcoholic fatty liver disease.
Food Sci Nutr. 2020 Jul; 8(7):3212-3224.FS

Abstract

Nonalcoholic fatty liver disease (NAFLD) is one of the prevalent and typical chronic liver diseases. In this study, we extracted a novel Angelica sinensis polysaccharide (ASP) with low molecular weight (MW) of 3.2 kDa through optimized "one-step" purification process. The major monosaccharide components of ASP were mannose, rhamnose, glucuronic acid, galactose, arabinose, and xylose with weight ratio of 0.23:0.17:14.41:0.39:1.68:0.87, respectively. Herein, "small" ASP could serve as an effective therapeutic option for NAFLD both in free fatty acid-induced L02 models and in high-fat diet-induced mice models. Results revealed that low MW ASP dose-dependently decreased TG, TC in vitro and TG, TC, ALT, HDL-C, and LDL-C in vivo. Oil Red O-positive area and Nile red fluorescence intensity decreased in ASP treatment groups both in vitro and in vivo which suggested ASP could reduce lipid accumulation and fatty regeneration. Hematoxylin-eosin staining results shown a decrease in hepatocytes ballooning indicating that ASP could ameliorate liver lipid degeneration. Briefly, a novel polysaccharide with low MW was successfully obtained which can prospectively act as NAFLD therapy.

Authors+Show Affiliations

Key Lab for Drug Delivery and Tissue Regeneration Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources School of Pharmacy Jiangsu University Zhenjiang China.Key Lab for Drug Delivery and Tissue Regeneration Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources School of Pharmacy Jiangsu University Zhenjiang China.Key Lab for Drug Delivery and Tissue Regeneration Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources School of Pharmacy Jiangsu University Zhenjiang China.Key Lab for Drug Delivery and Tissue Regeneration Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources School of Pharmacy Jiangsu University Zhenjiang China.Key Lab for Drug Delivery and Tissue Regeneration Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources School of Pharmacy Jiangsu University Zhenjiang China.Key Lab for Drug Delivery and Tissue Regeneration Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources School of Pharmacy Jiangsu University Zhenjiang China.Key Lab for Drug Delivery and Tissue Regeneration Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources School of Pharmacy Jiangsu University Zhenjiang China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32724586

Citation

Ma, Ping, et al. "Extraction and Structural Analysis of Angelica Sinensis Polysaccharide With Low Molecular Weight and Its Lipid-lowering Effect On Nonalcoholic Fatty Liver Disease." Food Science & Nutrition, vol. 8, no. 7, 2020, pp. 3212-3224.
Ma P, Sun C, Li W, et al. Extraction and structural analysis of Angelica sinensis polysaccharide with low molecular weight and its lipid-lowering effect on nonalcoholic fatty liver disease. Food Sci Nutr. 2020;8(7):3212-3224.
Ma, P., Sun, C., Li, W., Deng, W., Adu-Frimpong, M., Yu, J., & Xu, X. (2020). Extraction and structural analysis of Angelica sinensis polysaccharide with low molecular weight and its lipid-lowering effect on nonalcoholic fatty liver disease. Food Science & Nutrition, 8(7), 3212-3224. https://doi.org/10.1002/fsn3.1581
Ma P, et al. Extraction and Structural Analysis of Angelica Sinensis Polysaccharide With Low Molecular Weight and Its Lipid-lowering Effect On Nonalcoholic Fatty Liver Disease. Food Sci Nutr. 2020;8(7):3212-3224. PubMed PMID: 32724586.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extraction and structural analysis of Angelica sinensis polysaccharide with low molecular weight and its lipid-lowering effect on nonalcoholic fatty liver disease. AU - Ma,Ping, AU - Sun,Congyong, AU - Li,Wenjing, AU - Deng,Wenwen, AU - Adu-Frimpong,Michael, AU - Yu,Jiangnan, AU - Xu,Ximing, Y1 - 2020/05/18/ PY - 2020/01/14/received PY - 2020/03/22/revised PY - 2020/03/27/accepted PY - 2020/7/30/entrez PY - 2020/7/30/pubmed PY - 2020/7/30/medline KW - Angelica sinensis polysaccharide KW - NAFLD KW - lipid metabolism KW - low molecular weight KW - optimized purification process SP - 3212 EP - 3224 JF - Food science & nutrition JO - Food Sci Nutr VL - 8 IS - 7 N2 - Nonalcoholic fatty liver disease (NAFLD) is one of the prevalent and typical chronic liver diseases. In this study, we extracted a novel Angelica sinensis polysaccharide (ASP) with low molecular weight (MW) of 3.2 kDa through optimized "one-step" purification process. The major monosaccharide components of ASP were mannose, rhamnose, glucuronic acid, galactose, arabinose, and xylose with weight ratio of 0.23:0.17:14.41:0.39:1.68:0.87, respectively. Herein, "small" ASP could serve as an effective therapeutic option for NAFLD both in free fatty acid-induced L02 models and in high-fat diet-induced mice models. Results revealed that low MW ASP dose-dependently decreased TG, TC in vitro and TG, TC, ALT, HDL-C, and LDL-C in vivo. Oil Red O-positive area and Nile red fluorescence intensity decreased in ASP treatment groups both in vitro and in vivo which suggested ASP could reduce lipid accumulation and fatty regeneration. Hematoxylin-eosin staining results shown a decrease in hepatocytes ballooning indicating that ASP could ameliorate liver lipid degeneration. Briefly, a novel polysaccharide with low MW was successfully obtained which can prospectively act as NAFLD therapy. SN - 2048-7177 UR - https://www.unboundmedicine.com/medline/citation/32724586/Extraction_and_structural_analysis_of_Angelica_sinensis_polysaccharide_with_low_molecular_weight_and_its_lipid-lowering_effect_on_nonalcoholic_fatty_liver_disease. L2 - https://doi.org/10.1002/fsn3.1581 DB - PRIME DP - Unbound Medicine ER -
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