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GC-MS analysis, antimicrobial, antioxidant, antilipoxygenase and cytotoxic activities of Jacaranda mimosifolia methanol leaf extracts and fractions.
PLoS One. 2020; 15(7):e0236319.Plos

Abstract

Jacaranda mimosifolia trees are grown in frost-free regions globally. The aim of this study was to evaluate the methanol crude extract and various fractions of increasing polarity of J. mimosifolia leaves for bioactive metabolites, as well as antimicrobial, antioxidant and anticancer activities. The anti-inflammatory potential of the various fractions of J. mimosifolia leaf extract was studied via the lipoxygenase (LOX) inhibitory assay. Methanol crude extract (ME), derived fractions extracted with chloroform (CF) and ethyl acetate (EAF), and residual aqueous extract (AE) of dried J. mimosifolia leaves were assayed for polyphenolic compounds, their antioxidant, antimicrobial and lipoxygenase (LOX) inhibitory activities, and anticancer properties. Polyphenolic compounds were determined via HPLC while phytochemicals (total phenolics, flavonoids, tannins and ortho-diphenol contents), antioxidant activities (DPPH, hydrogen peroxideperoxide, hydroxyl and superoxide radical anions) and LOX were measured via spectrophotometry. Methanol extracts and various fractions were evaluated for antibacterial activities against Bacillus subtilis, Klebsiella pneumonia, Pseudomonas aeruginosa and Staphylococcus aureus. Antifungal potential of the fractions was tested against three species: Aspergillus flavus, Aspergillus fumigatus and Fusarium oxysporum. The highest values for total phenolic content (TPC), total flavonoid content (TFC), flavonols, tannins and ortho-diphenols were in the ME, followed by CF > EAF > AE. ME also had the highest antioxidant activity with EC50 values 48±1.3, 45±2.4, 42±1.3 and 46±1.3 μg/mL based on the DPPH, hydrogen peroxide, hydroxyl radical and superoxide radical assays, respectively. TPC and TFC showed a significant, strong and positive correlation with the values for each of these antioxidant activities. ME exhibited anti-inflammatory potential based on its LOX inhibitory activity (IC50 = 1.3 μg/mL). ME also had the maximum antibacterial and antifungal potential, followed by EAF > CF > AE. Furthermore, ME showed the strongest cytotoxic effect (EC50 = 10.7 and 17.3 μg/mL) against human hormone-dependent prostate carcinoma (LnCaP) and human lung carcinoma (LU-1) cell lines, respectively. Bioactive compounds present in leaf methanol extracts of J. mimosifolia were identified using gas chromatography-mass spectrometry (GC-MS). Fifteen compounds were identified including phenolic and alcoholic compounds, as well as fatty acids. Our results suggest that J. mimosifolia leaves are a good source of natural products with antioxidant, anti-inflammatory and anti-cancer properties for potential therapeutic, nutraceutical and functional food applications.

Authors+Show Affiliations

Department of Biosciences, COMSATS University, Islamabad, Pakistan.Plant Breeding Institute, Sydney Institute of Agriculture, University of Sydney, Sydney, NSW, Australia.Department of Biosciences, University of Wah, Wah Cantt, Pakistan.Department of Biosciences, COMSATS University, Islamabad, Pakistan.Department of Biosciences, COMSATS University, Islamabad, Pakistan.Department of Biosciences, COMSATS University, Islamabad, Pakistan.Department of Biosciences, COMSATS University, Islamabad, Pakistan.Department of Botany, University of Peshawar, Peshawar, Pakistan.Department of Biotechnology, COMSATS University Islamabad, Abbotabad Campus, Abbotabad, Pakistan.Department of Computer Science, COMSATS University Islamabad, Vehari Campus, Vehari, Pakistan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32726328

Citation

Naz, Rabia, et al. "GC-MS Analysis, Antimicrobial, Antioxidant, Antilipoxygenase and Cytotoxic Activities of Jacaranda Mimosifolia Methanol Leaf Extracts and Fractions." PloS One, vol. 15, no. 7, 2020, pp. e0236319.
Naz R, Roberts TH, Bano A, et al. GC-MS analysis, antimicrobial, antioxidant, antilipoxygenase and cytotoxic activities of Jacaranda mimosifolia methanol leaf extracts and fractions. PLoS One. 2020;15(7):e0236319.
Naz, R., Roberts, T. H., Bano, A., Nosheen, A., Yasmin, H., Hassan, M. N., Keyani, R., Ullah, S., Khan, W., & Anwar, Z. (2020). GC-MS analysis, antimicrobial, antioxidant, antilipoxygenase and cytotoxic activities of Jacaranda mimosifolia methanol leaf extracts and fractions. PloS One, 15(7), e0236319. https://doi.org/10.1371/journal.pone.0236319
Naz R, et al. GC-MS Analysis, Antimicrobial, Antioxidant, Antilipoxygenase and Cytotoxic Activities of Jacaranda Mimosifolia Methanol Leaf Extracts and Fractions. PLoS One. 2020;15(7):e0236319. PubMed PMID: 32726328.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GC-MS analysis, antimicrobial, antioxidant, antilipoxygenase and cytotoxic activities of Jacaranda mimosifolia methanol leaf extracts and fractions. AU - Naz,Rabia, AU - Roberts,Thomas H, AU - Bano,Asghari, AU - Nosheen,Asia, AU - Yasmin,Humaira, AU - Hassan,Muhammad Nadeem, AU - Keyani,Rumana, AU - Ullah,Sami, AU - Khan,Wajiha, AU - Anwar,Zahid, Y1 - 2020/07/29/ PY - 2020/03/31/received PY - 2020/07/01/accepted PY - 2020/7/30/entrez PY - 2020/7/30/pubmed PY - 2020/9/24/medline SP - e0236319 EP - e0236319 JF - PloS one JO - PLoS One VL - 15 IS - 7 N2 - Jacaranda mimosifolia trees are grown in frost-free regions globally. The aim of this study was to evaluate the methanol crude extract and various fractions of increasing polarity of J. mimosifolia leaves for bioactive metabolites, as well as antimicrobial, antioxidant and anticancer activities. The anti-inflammatory potential of the various fractions of J. mimosifolia leaf extract was studied via the lipoxygenase (LOX) inhibitory assay. Methanol crude extract (ME), derived fractions extracted with chloroform (CF) and ethyl acetate (EAF), and residual aqueous extract (AE) of dried J. mimosifolia leaves were assayed for polyphenolic compounds, their antioxidant, antimicrobial and lipoxygenase (LOX) inhibitory activities, and anticancer properties. Polyphenolic compounds were determined via HPLC while phytochemicals (total phenolics, flavonoids, tannins and ortho-diphenol contents), antioxidant activities (DPPH, hydrogen peroxideperoxide, hydroxyl and superoxide radical anions) and LOX were measured via spectrophotometry. Methanol extracts and various fractions were evaluated for antibacterial activities against Bacillus subtilis, Klebsiella pneumonia, Pseudomonas aeruginosa and Staphylococcus aureus. Antifungal potential of the fractions was tested against three species: Aspergillus flavus, Aspergillus fumigatus and Fusarium oxysporum. The highest values for total phenolic content (TPC), total flavonoid content (TFC), flavonols, tannins and ortho-diphenols were in the ME, followed by CF > EAF > AE. ME also had the highest antioxidant activity with EC50 values 48±1.3, 45±2.4, 42±1.3 and 46±1.3 μg/mL based on the DPPH, hydrogen peroxide, hydroxyl radical and superoxide radical assays, respectively. TPC and TFC showed a significant, strong and positive correlation with the values for each of these antioxidant activities. ME exhibited anti-inflammatory potential based on its LOX inhibitory activity (IC50 = 1.3 μg/mL). ME also had the maximum antibacterial and antifungal potential, followed by EAF > CF > AE. Furthermore, ME showed the strongest cytotoxic effect (EC50 = 10.7 and 17.3 μg/mL) against human hormone-dependent prostate carcinoma (LnCaP) and human lung carcinoma (LU-1) cell lines, respectively. Bioactive compounds present in leaf methanol extracts of J. mimosifolia were identified using gas chromatography-mass spectrometry (GC-MS). Fifteen compounds were identified including phenolic and alcoholic compounds, as well as fatty acids. Our results suggest that J. mimosifolia leaves are a good source of natural products with antioxidant, anti-inflammatory and anti-cancer properties for potential therapeutic, nutraceutical and functional food applications. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/32726328/GC_MS_analysis_antimicrobial_antioxidant_antilipoxygenase_and_cytotoxic_activities_of_Jacaranda_mimosifolia_methanol_leaf_extracts_and_fractions_ L2 - https://dx.plos.org/10.1371/journal.pone.0236319 DB - PRIME DP - Unbound Medicine ER -