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Dietary Antioxidants Significantly Reduced Phorbol Myristate Acetate Induced Oxidative Stress of Peripheral Blood Mononuclear Cells of Patients with Rheumatoid Arthritis.
Curr Rheumatol Rev. 2021; 17(1):81-87.CR

Abstract

BACKGROUND

Rheumatoid arthritis (RA) is an autoimmune disease responsible for maximum human morbidity in modern life, whereas oxidative stress is the ultimate potential biomarker for determining disease activity in patients with RA.

OBJECTIVE

The present study scientifically validated the effectiveness of antioxidants commonly present in different food supplements to neutralize the free radicals mediated oxidative stress in isolated peripheral blood mononuclear lymphocytes (PBML) of patients with RA.

METHODS

The study population included patients with Rheumatoid arthritis, RA (n =15), who fulfilled the American College of Rheumatology criteria for RA. Peripheral blood was collected, and isolated mononuclear lymphocyte cells (PBML) were pretreated with phorbol myristate acetate (PMS) and furthermore, incubated with different concentrations of Naringenin, β carotene and Nacetyl cysteine (NAC) in an ex vivo condition. The resultant cell lysate was used for further studies for the determination of other oxidative biomarkers. The increase of superoxide and nitric oxide production was observed when PBML was treated PMS.

RESULTS

Importantly, the increased oxidative stress was effectively decreased by the selected plantderived compounds β-carotene and naringenin.

CONCLUSION

The study scientifically evaluated the efficacy of the molecules validated by one-way ANOVA, followed by Dunnett's post hoc test of significance. Collectively, our results indicate that both β carotene and naringenin may be a promising non-toxic food supplement in attenuating the oxidative stress associated pathology in RA, meriting further pharmacological studies on other inflammatory cells like neutrophils.

Authors+Show Affiliations

Department of Pharmacology, Himalayan Pharmacy Institute, Majhitar, Rangpo, East Sikkim 737136, Sikkim, India.Department of Medicine, Sikkim Manipal Institute of Medical Science, 5th Mile Tadong, Gangtok 737102, Sikkim, India.Department of Pharmaceutical Technology, Jadavpur University, 188B Raja SC Mullick Road, Kolkata 700032, West Bengal, India.Division of Pharmacology, Guru Nanak Institute of Pharmaceutical Science and Technology, Sodepur, Pin- 700114, West Bengal, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32729420

Citation

Chetia, Purbajit, et al. "Dietary Antioxidants Significantly Reduced Phorbol Myristate Acetate Induced Oxidative Stress of Peripheral Blood Mononuclear Cells of Patients With Rheumatoid Arthritis." Current Rheumatology Reviews, vol. 17, no. 1, 2021, pp. 81-87.
Chetia P, Khandelwal B, Haldar PK, et al. Dietary Antioxidants Significantly Reduced Phorbol Myristate Acetate Induced Oxidative Stress of Peripheral Blood Mononuclear Cells of Patients with Rheumatoid Arthritis. Curr Rheumatol Rev. 2021;17(1):81-87.
Chetia, P., Khandelwal, B., Haldar, P. K., & Bala, A. (2021). Dietary Antioxidants Significantly Reduced Phorbol Myristate Acetate Induced Oxidative Stress of Peripheral Blood Mononuclear Cells of Patients with Rheumatoid Arthritis. Current Rheumatology Reviews, 17(1), 81-87. https://doi.org/10.2174/1573397116999200729154954
Chetia P, et al. Dietary Antioxidants Significantly Reduced Phorbol Myristate Acetate Induced Oxidative Stress of Peripheral Blood Mononuclear Cells of Patients With Rheumatoid Arthritis. Curr Rheumatol Rev. 2021;17(1):81-87. PubMed PMID: 32729420.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary Antioxidants Significantly Reduced Phorbol Myristate Acetate Induced Oxidative Stress of Peripheral Blood Mononuclear Cells of Patients with Rheumatoid Arthritis. AU - Chetia,Purbajit, AU - Khandelwal,Bidita, AU - Haldar,Pallab Kanti, AU - Bala,Asis, PY - 2020/04/01/received PY - 2020/06/24/revised PY - 2020/06/29/accepted PY - 2020/7/31/pubmed PY - 2021/11/25/medline PY - 2020/7/31/entrez KW - Rheumatoid arthritis KW - antioxidants KW - mononuclear lymphocytes KW - naringenin KW - oxidative stress KW - β carotene and Nacetyl cysteine (NAC) SP - 81 EP - 87 JF - Current rheumatology reviews JO - Curr Rheumatol Rev VL - 17 IS - 1 N2 - BACKGROUND: Rheumatoid arthritis (RA) is an autoimmune disease responsible for maximum human morbidity in modern life, whereas oxidative stress is the ultimate potential biomarker for determining disease activity in patients with RA. OBJECTIVE: The present study scientifically validated the effectiveness of antioxidants commonly present in different food supplements to neutralize the free radicals mediated oxidative stress in isolated peripheral blood mononuclear lymphocytes (PBML) of patients with RA. METHODS: The study population included patients with Rheumatoid arthritis, RA (n =15), who fulfilled the American College of Rheumatology criteria for RA. Peripheral blood was collected, and isolated mononuclear lymphocyte cells (PBML) were pretreated with phorbol myristate acetate (PMS) and furthermore, incubated with different concentrations of Naringenin, β carotene and Nacetyl cysteine (NAC) in an ex vivo condition. The resultant cell lysate was used for further studies for the determination of other oxidative biomarkers. The increase of superoxide and nitric oxide production was observed when PBML was treated PMS. RESULTS: Importantly, the increased oxidative stress was effectively decreased by the selected plantderived compounds β-carotene and naringenin. CONCLUSION: The study scientifically evaluated the efficacy of the molecules validated by one-way ANOVA, followed by Dunnett's post hoc test of significance. Collectively, our results indicate that both β carotene and naringenin may be a promising non-toxic food supplement in attenuating the oxidative stress associated pathology in RA, meriting further pharmacological studies on other inflammatory cells like neutrophils. SN - 1875-6360 UR - https://www.unboundmedicine.com/medline/citation/32729420/Dietary_Antioxidants_Significantly_Reduced_Phorbol_Myristate_Acetate_Induced_Oxidative_Stress_of_Peripheral_Blood_Mononuclear_Cells_of_Patients_with_Rheumatoid_Arthritis_ DB - PRIME DP - Unbound Medicine ER -