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MicroRNA Expression Signature in Mild Cognitive Impairment Due to Alzheimer's Disease.
Mol Neurobiol. 2020 Nov; 57(11):4408-4416.MN

Abstract

Mild cognitive impairment (MCI) defines an intermediate state between normal ageing and dementia, including Alzheimer's disease (AD). Identification of MCI subjects who will progress to AD (MCI-AD) is today of crucial importance, especially in light of the possible development of new pathogenic therapies. Several evidences suggest that miRNAs could play relevant roles in the biogenesis of AD, and the links between selected miRNAs and specific pathogenic aspects have been partly explored. In this study, we analysed the composition of microRNA transcriptome in blood, serum and cerebrospinal fluid samples from MCI-AD subjects, from an enriched small RNA library. Real-time qPCR from MCI-AD and AD patients and normal controls was performed to profile miRNA expression. In particular, four microRNAs, hsa-mir-5588-5p, hsa-mir-3658, hsa-mir-567 and hsa-mir-3908, among all selected microRNAs, are dysregulated. Hsa-mir-567 was found to be differentially expressed in cerebrospinal fluid samples, blood and serum from MCI-AD patients, showing the highest fold change and statistical significance. Target prediction analysis have been performed to evaluate mRNAs whose expression was controlled by miRNAs found to be dysregulated here, showing that hsa-mir-567 target genes are functionally active in neuronal cells. We propose that miRNA profiles found in samples from MCI-AD patients might be relevant for a better understanding of AD-related cognitive decline and could lead to set up suitable and potential biomarkers for MCI-AD progression to AD.

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Authors+Show Affiliations

De Felice B
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies (DISTABIF), University of Campania "Luigi Vanvitelli", Via Vivaldi 43, 81100, Caserta, Italy. bruna.defelice@unicampania.it.
Montanino C
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies (DISTABIF), University of Campania "Luigi Vanvitelli", Via Vivaldi 43, 81100, Caserta, Italy.
Oliva M
Department of Advanced Medical and Surgical Sciences, University of Campania "L. Vanvitelli", Naples, Italy.
Bonavita S
Department of Advanced Medical and Surgical Sciences, University of Campania "L. Vanvitelli", Naples, Italy.
Di Onofrio V
Department of Sciences and Technologies, University of Naples "Parthenope", Naples, Italy.
Coppola C
Department of Advanced Medical and Surgical Sciences, University of Campania "L. Vanvitelli", Naples, Italy.

MeSH

AgedAged, 80 and overAlzheimer DiseaseCase-Control StudiesCognitive DysfunctionFemaleGene Expression ProfilingGene Expression RegulationGene OntologyGene Regulatory NetworksHumansLeukocytesMaleMicroRNAsMiddle AgedProtein Interaction Maps

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32737762

Citation

De Felice, Bruna, et al. "MicroRNA Expression Signature in Mild Cognitive Impairment Due to Alzheimer's Disease." Molecular Neurobiology, vol. 57, no. 11, 2020, pp. 4408-4416.
De Felice B, Montanino C, Oliva M, et al. MicroRNA Expression Signature in Mild Cognitive Impairment Due to Alzheimer's Disease. Mol Neurobiol. 2020;57(11):4408-4416.
De Felice, B., Montanino, C., Oliva, M., Bonavita, S., Di Onofrio, V., & Coppola, C. (2020). MicroRNA Expression Signature in Mild Cognitive Impairment Due to Alzheimer's Disease. Molecular Neurobiology, 57(11), 4408-4416. https://doi.org/10.1007/s12035-020-02029-7
De Felice B, et al. MicroRNA Expression Signature in Mild Cognitive Impairment Due to Alzheimer's Disease. Mol Neurobiol. 2020;57(11):4408-4416. PubMed PMID: 32737762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MicroRNA Expression Signature in Mild Cognitive Impairment Due to Alzheimer's Disease. AU - De Felice,Bruna, AU - Montanino,Concetta, AU - Oliva,Mariano, AU - Bonavita,Simona, AU - Di Onofrio,Valeria, AU - Coppola,Cinzia, Y1 - 2020/07/31/ PY - 2020/01/31/received PY - 2020/07/22/accepted PY - 2020/8/2/pubmed PY - 2021/6/30/medline PY - 2020/8/2/entrez KW - Alzheimer’s disease (AD) KW - Hsa-mir-567 KW - Mild cognitive impairment (MCI) KW - microRNAs SP - 4408 EP - 4416 JF - Molecular neurobiology JO - Mol Neurobiol VL - 57 IS - 11 N2 - Mild cognitive impairment (MCI) defines an intermediate state between normal ageing and dementia, including Alzheimer's disease (AD). Identification of MCI subjects who will progress to AD (MCI-AD) is today of crucial importance, especially in light of the possible development of new pathogenic therapies. Several evidences suggest that miRNAs could play relevant roles in the biogenesis of AD, and the links between selected miRNAs and specific pathogenic aspects have been partly explored. In this study, we analysed the composition of microRNA transcriptome in blood, serum and cerebrospinal fluid samples from MCI-AD subjects, from an enriched small RNA library. Real-time qPCR from MCI-AD and AD patients and normal controls was performed to profile miRNA expression. In particular, four microRNAs, hsa-mir-5588-5p, hsa-mir-3658, hsa-mir-567 and hsa-mir-3908, among all selected microRNAs, are dysregulated. Hsa-mir-567 was found to be differentially expressed in cerebrospinal fluid samples, blood and serum from MCI-AD patients, showing the highest fold change and statistical significance. Target prediction analysis have been performed to evaluate mRNAs whose expression was controlled by miRNAs found to be dysregulated here, showing that hsa-mir-567 target genes are functionally active in neuronal cells. We propose that miRNA profiles found in samples from MCI-AD patients might be relevant for a better understanding of AD-related cognitive decline and could lead to set up suitable and potential biomarkers for MCI-AD progression to AD. SN - 1559-1182 UR - https://www.unboundmedicine.com/medline/citation/32737762/MicroRNA_Expression_Signature_in_Mild_Cognitive_Impairment_Due_to_Alzheimer's_Disease_ DB - PRIME DP - Unbound Medicine ER -