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A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition.
Cell Host Microbe. 2020 09 09; 28(3):486-496.e6.CH

Abstract

There is an urgent need for vaccines and therapeutics to prevent and treat COVID-19. Rapid SARS-CoV-2 countermeasure development is contingent on the availability of robust, scalable, and readily deployable surrogate viral assays to screen antiviral humoral responses, define correlates of immune protection, and down-select candidate antivirals. Here, we generate a highly infectious recombinant vesicular stomatitis virus (VSV) bearing the SARS-CoV-2 spike glycoprotein S as its sole entry glycoprotein and show that this recombinant virus, rVSV-SARS-CoV-2 S, closely resembles SARS-CoV-2 in its entry-related properties. The neutralizing activities of a large panel of COVID-19 convalescent sera can be assessed in a high-throughput fluorescent reporter assay with rVSV-SARS-CoV-2 S, and neutralization of rVSV-SARS-CoV-2 S and authentic SARS-CoV-2 by spike-specific antibodies in these antisera is highly correlated. Our findings underscore the utility of rVSV-SARS-CoV-2 S for the development of spike-specific therapeutics and for mechanistic studies of viral entry and its inhibition.

Authors+Show Affiliations

Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA.Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Department of Chemistry and Life Science, United States Military Academy at West Point, West Point, NY 10996, USA.Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.Department of Chemistry and Life Science, United States Military Academy at West Point, West Point, NY 10996, USA.Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA.Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA; The Geneva Foundation, 917 Pacific Avenue, Tacoma, WA 98402, USA. Electronic address: andrew.s.herbert4.ctr@mail.mil.Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA. Electronic address: kartik.chandran@einsteinmed.org.Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA. Electronic address: rohit.jangra@einsteinmed.org.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32738193

Citation

Dieterle, M Eugenia, et al. "A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition." Cell Host & Microbe, vol. 28, no. 3, 2020, pp. 486-496.e6.
Dieterle ME, Haslwanter D, Bortz RH, et al. A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition. Cell Host Microbe. 2020;28(3):486-496.e6.
Dieterle, M. E., Haslwanter, D., Bortz, R. H., Wirchnianski, A. S., Lasso, G., Vergnolle, O., Abbasi, S. A., Fels, J. M., Laudermilch, E., Florez, C., Mengotto, A., Kimmel, D., Malonis, R. J., Georgiev, G., Quiroz, J., Barnhill, J., Pirofski, L. A., Daily, J. P., Dye, J. M., ... Jangra, R. K. (2020). A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition. Cell Host & Microbe, 28(3), 486-e6. https://doi.org/10.1016/j.chom.2020.06.020
Dieterle ME, et al. A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition. Cell Host Microbe. 2020 09 9;28(3):486-496.e6. PubMed PMID: 32738193.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition. AU - Dieterle,M Eugenia, AU - Haslwanter,Denise, AU - Bortz,Robert H,3rd AU - Wirchnianski,Ariel S, AU - Lasso,Gorka, AU - Vergnolle,Olivia, AU - Abbasi,Shawn A, AU - Fels,J Maximilian, AU - Laudermilch,Ethan, AU - Florez,Catalina, AU - Mengotto,Amanda, AU - Kimmel,Duncan, AU - Malonis,Ryan J, AU - Georgiev,George, AU - Quiroz,Jose, AU - Barnhill,Jason, AU - Pirofski,Liise-Anne, AU - Daily,Johanna P, AU - Dye,John M, AU - Lai,Jonathan R, AU - Herbert,Andrew S, AU - Chandran,Kartik, AU - Jangra,Rohit K, Y1 - 2020/07/03/ PY - 2020/05/19/received PY - 2020/06/16/revised PY - 2020/06/23/accepted PY - 2020/8/2/pubmed PY - 2020/9/22/medline PY - 2020/8/2/entrez KW - ACE2 KW - COVID-19 KW - SARS-CoV-2 KW - VSV KW - antiviral drugs KW - convalescent plasma KW - neutralization assay KW - neutralizing antibody KW - serology KW - surrogate SP - 486 EP - 496.e6 JF - Cell host & microbe JO - Cell Host Microbe VL - 28 IS - 3 N2 - There is an urgent need for vaccines and therapeutics to prevent and treat COVID-19. Rapid SARS-CoV-2 countermeasure development is contingent on the availability of robust, scalable, and readily deployable surrogate viral assays to screen antiviral humoral responses, define correlates of immune protection, and down-select candidate antivirals. Here, we generate a highly infectious recombinant vesicular stomatitis virus (VSV) bearing the SARS-CoV-2 spike glycoprotein S as its sole entry glycoprotein and show that this recombinant virus, rVSV-SARS-CoV-2 S, closely resembles SARS-CoV-2 in its entry-related properties. The neutralizing activities of a large panel of COVID-19 convalescent sera can be assessed in a high-throughput fluorescent reporter assay with rVSV-SARS-CoV-2 S, and neutralization of rVSV-SARS-CoV-2 S and authentic SARS-CoV-2 by spike-specific antibodies in these antisera is highly correlated. Our findings underscore the utility of rVSV-SARS-CoV-2 S for the development of spike-specific therapeutics and for mechanistic studies of viral entry and its inhibition. SN - 1934-6069 UR - https://www.unboundmedicine.com/medline/citation/32738193/A_Replication_Competent_Vesicular_Stomatitis_Virus_for_Studies_of_SARS_CoV_2_Spike_Mediated_Cell_Entry_and_Its_Inhibition_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1931-3128(20)30361-9 DB - PRIME DP - Unbound Medicine ER -