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Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques.
Arch Virol. 2020 Oct; 165(10):2373-2377.AV

Abstract

In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.

Authors+Show Affiliations

Louisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, River Rd, Room 1043, Baton Rouge, LA, 70803, USA.United States Department of the Interior, U. S. Geological Survey, National Wildlife Health Center, 6006, Schroeder Road, Madison, WI, 53711, USA.Department of Diagnostic Medicine/Pathobiology, Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD), Kansas State University, K-224B Mosier Hall, Manhattan, KS, 66506, USA.Louisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, River Rd, Room 1043, Baton Rouge, LA, 70803, USA.Louisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, River Rd, Room 1043, Baton Rouge, LA, 70803, USA.University of Kentucky Veterinary Diagnostic Laboratory, Department of Veterinary Science, University of Kentucky, Lexington, KY, 40511, USA.Louisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, River Rd, Room 1043, Baton Rouge, LA, 70803, USA.Louisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, River Rd, Room 1043, Baton Rouge, LA, 70803, USA. balasuriya1@lsu.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32761270

Citation

Carossino, Mariano, et al. "Detection of SARS-CoV-2 By RNAscope® in Situ Hybridization and Immunohistochemistry Techniques." Archives of Virology, vol. 165, no. 10, 2020, pp. 2373-2377.
Carossino M, Ip HS, Richt JA, et al. Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques. Arch Virol. 2020;165(10):2373-2377.
Carossino, M., Ip, H. S., Richt, J. A., Shultz, K., Harper, K., Loynachan, A. T., Del Piero, F., & Balasuriya, U. B. R. (2020). Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques. Archives of Virology, 165(10), 2373-2377. https://doi.org/10.1007/s00705-020-04737-w
Carossino M, et al. Detection of SARS-CoV-2 By RNAscope® in Situ Hybridization and Immunohistochemistry Techniques. Arch Virol. 2020;165(10):2373-2377. PubMed PMID: 32761270.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques. AU - Carossino,Mariano, AU - Ip,Hon S, AU - Richt,Juergen A, AU - Shultz,Kendra, AU - Harper,Kimberly, AU - Loynachan,Alan T, AU - Del Piero,Fabio, AU - Balasuriya,Udeni B R, Y1 - 2020/08/05/ PY - 2020/05/16/received PY - 2020/06/18/accepted PY - 2020/8/8/pubmed PY - 2020/9/22/medline PY - 2020/8/8/entrez SP - 2373 EP - 2377 JF - Archives of virology JO - Arch Virol VL - 165 IS - 10 N2 - In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2. SN - 1432-8798 UR - https://www.unboundmedicine.com/medline/citation/32761270/Detection_of_SARS_CoV_2_by_RNAscope®_in_situ_hybridization_and_immunohistochemistry_techniques_ L2 - https://dx.doi.org/10.1007/s00705-020-04737-w DB - PRIME DP - Unbound Medicine ER -