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Circulating endothelial progenitors are increased in COVID-19 patients and correlate with SARS-CoV-2 RNA in severe cases.
J Thromb Haemost. 2020 Aug 06 [Online ahead of print]JT

Abstract

BACKGROUND

During the course of COVID-19, the disease caused by the new coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), thrombotic phenomena and/or diffuse vascular damage are frequent, and viral elements have been observed within endothelial cells.

OBJECTIVES

CD146 + circulating endothelial cells (CD146 + CECs) and their progenitors (CEPs) are increased in cardiovascular, thrombotic, infectious, and cancer diseases. The present study was designed to investigate their kinetics in novel coronavirus (COVID-19) patients.

METHODS

We used a validated flow cytometry procedure to enumerate viable and apoptotic CD146 + CECs and CEPs in COVID-19 patients during the course of the disease and in patients who recovered.

RESULTS

Viable CEPs per milliliter were significantly increased in COVID-19 patients compared with healthy controls. This increase was observed in patients with mild symptoms and not further augmented in patients with severe symptoms. In patients who recovered, CEPs decreased, but were in a range still significantly higher than normal controls. Regarding mature CD146 + CECs, in COVID-19 patients, their absolute number was similar to those observed in healthy controls, but the viable/apoptotic CD146 + CEC ratio was significantly different. Both mild and severe COVID-19 patients had significantly less apoptotic CD146 + CECs compared with healthy controls. Patients who recovered had significantly less CD146 + CECs per milliliter when compared with controls as well as to mild and severe COVID-19 patients. A positive correlation was found between the copies of SARS-CoV-2 RNA in the cellular fraction and apoptotic CEPs per milliliter in severe COVID-19 patients.

CONCLUSIONS

CD146 + CECs and CEPs might be investigated as candidate biomarkers of endothelial damage in COVID-19 patients.

Authors+Show Affiliations

Laboratory of Hematology-Oncology, European Institute of Oncology IRCCS, Milan, Italy.ASST Fatebenefratelli Sacco, Department of Biomedical and Clinical Sciences, "Luigi Sacco" Hospital, University of Milan, Milan, Italy.Laboratory of Hematology-Oncology, European Institute of Oncology IRCCS, Milan, Italy.Laboratory of Hematology-Oncology, European Institute of Oncology IRCCS, Milan, Italy.Laboratory of Hematology-Oncology, European Institute of Oncology IRCCS, Milan, Italy.Laboratory of Hematology-Oncology, European Institute of Oncology IRCCS, Milan, Italy.ASST Fatebenefratelli Sacco, Department of Biomedical and Clinical Sciences, "Luigi Sacco" Hospital, University of Milan, Milan, Italy.ASST Fatebenefratelli Sacco, Department of Biomedical and Clinical Sciences, "Luigi Sacco" Hospital, University of Milan, Milan, Italy.ASST Fatebenefratelli Sacco, Department of Biomedical and Clinical Sciences, "Luigi Sacco" Hospital, University of Milan, Milan, Italy.ASST Fatebenefratelli Sacco, Department of Biomedical and Clinical Sciences, "Luigi Sacco" Hospital, University of Milan, Milan, Italy.ASST Fatebenefratelli Sacco, Department of Biomedical and Clinical Sciences, "Luigi Sacco" Hospital, University of Milan, Milan, Italy.Laboratory of Hematology-Oncology, European Institute of Oncology IRCCS, Milan, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32762140

Citation

Mancuso, Patrizia, et al. "Circulating Endothelial Progenitors Are Increased in COVID-19 Patients and Correlate With SARS-CoV-2 RNA in Severe Cases." Journal of Thrombosis and Haemostasis : JTH, 2020.
Mancuso P, Gidaro A, Gregato G, et al. Circulating endothelial progenitors are increased in COVID-19 patients and correlate with SARS-CoV-2 RNA in severe cases. J Thromb Haemost. 2020.
Mancuso, P., Gidaro, A., Gregato, G., Raveane, A., Cremonesi, P., Quarna, J., Caccia, S., Gusso, L., Rusconi, S., Giacomelli, A., Cogliati, C., & Bertolini, F. (2020). Circulating endothelial progenitors are increased in COVID-19 patients and correlate with SARS-CoV-2 RNA in severe cases. Journal of Thrombosis and Haemostasis : JTH. https://doi.org/10.1111/jth.15044
Mancuso P, et al. Circulating Endothelial Progenitors Are Increased in COVID-19 Patients and Correlate With SARS-CoV-2 RNA in Severe Cases. J Thromb Haemost. 2020 Aug 6; PubMed PMID: 32762140.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Circulating endothelial progenitors are increased in COVID-19 patients and correlate with SARS-CoV-2 RNA in severe cases. AU - Mancuso,Patrizia, AU - Gidaro,Antonio, AU - Gregato,Giuliana, AU - Raveane,Alessandro, AU - Cremonesi,Paola, AU - Quarna,Jessica, AU - Caccia,Sonia, AU - Gusso,Luca, AU - Rusconi,Stefano, AU - Giacomelli,Andrea, AU - Cogliati,Chiara, AU - Bertolini,Francesco, Y1 - 2020/08/06/ PY - 2020/05/13/received PY - 2020/06/26/revised PY - 2020/07/31/accepted PY - 2020/8/8/pubmed PY - 2020/8/8/medline PY - 2020/8/8/entrez KW - Covid-19 KW - SARS-CoV-2 KW - circulating endothelial cells KW - circulating endothelial progenitors KW - endothelial cells JF - Journal of thrombosis and haemostasis : JTH JO - J. Thromb. Haemost. N2 - BACKGROUND: During the course of COVID-19, the disease caused by the new coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), thrombotic phenomena and/or diffuse vascular damage are frequent, and viral elements have been observed within endothelial cells. OBJECTIVES: CD146 + circulating endothelial cells (CD146 + CECs) and their progenitors (CEPs) are increased in cardiovascular, thrombotic, infectious, and cancer diseases. The present study was designed to investigate their kinetics in novel coronavirus (COVID-19) patients. METHODS: We used a validated flow cytometry procedure to enumerate viable and apoptotic CD146 + CECs and CEPs in COVID-19 patients during the course of the disease and in patients who recovered. RESULTS: Viable CEPs per milliliter were significantly increased in COVID-19 patients compared with healthy controls. This increase was observed in patients with mild symptoms and not further augmented in patients with severe symptoms. In patients who recovered, CEPs decreased, but were in a range still significantly higher than normal controls. Regarding mature CD146 + CECs, in COVID-19 patients, their absolute number was similar to those observed in healthy controls, but the viable/apoptotic CD146 + CEC ratio was significantly different. Both mild and severe COVID-19 patients had significantly less apoptotic CD146 + CECs compared with healthy controls. Patients who recovered had significantly less CD146 + CECs per milliliter when compared with controls as well as to mild and severe COVID-19 patients. A positive correlation was found between the copies of SARS-CoV-2 RNA in the cellular fraction and apoptotic CEPs per milliliter in severe COVID-19 patients. CONCLUSIONS: CD146 + CECs and CEPs might be investigated as candidate biomarkers of endothelial damage in COVID-19 patients. SN - 1538-7836 UR - https://www.unboundmedicine.com/medline/citation/32762140/Circulating_endothelial_progenitors_are_increased_in_Covid-19_patients_and_correlate_with_SARS-CoV-2_RNA_in_severe_cases L2 - https://doi.org/10.1111/jth.15044 DB - PRIME DP - Unbound Medicine ER -
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