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[Comparative analysis of structural characteristics and epitopes in S proteins between SARS-CoV-2 and SARS-CoV].
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2020 May 25; 49(3):315-323.ZD

Abstract

OBJECTIVE

To provide data support for the study of pathogenic mechanism of SARS-CoV-2 at the molecular level, and provide suitable candidate targets for vaccine, antibody and drug research and development through comparative analysis for structural characteristics and epitopes of S protein of SARS-CoV-2 and SARS-CoV.

METHODS

Based on the reference sequences of S protein, physical and chemical properties, hydrophobicity, signal peptide, transmembrane region, domain, secondary structure, tertiary structure analysis and antigenic epitopes prediction were carried out. Meanwhile, the tissue expression, related pathways and reactome pathways of angiotensis Ⅰ converting enzyme 2 (ACE2) and C-type lectin domain family 4 member M (CLEC4M) receptors were analyzed.

RESULTS

The amino acid sequence of S protein of SARS-CoV-2 and SARS-CoV has a 75.80% consistency. The structural characteristics of the two coronaviruses are highly consistent, but the secondary structure and tertiary structure of SARS-CoV-2 is not as obvious as SARS-CoV. ACE2 and CLEC4M are expressed in alimentary system, heart, kidney, lung and placenta. The main related the pathways of renin-angiotensin system, protein digestion and absorption pathway, and the reactome pathways of metabolism of angiotensinogen to angiotensins, GPCR ligand binding, are related to typical symptoms of coronavirus disease 2019 induced by SARS-CoV-2. Three pairs of highly or completely homologous epitopes of S protein were obtained. The 600-605, 695-703 and 888-896 amino acid residues in SARS-CoV-2 were highly homologous with 586-591, 677-685 and 870-878 amino acid residues in SARS-CoV, respectively.

CONCLUSIONS

The similarity of S protein of SARS-CoV-2 and SARS-CoV determines that they have similar infection patterns and clinical manifestations. The candidate epitopes with high reliability can provide reference for virus diagnosis and vaccine development.

Authors+Show Affiliations

Key Laboratory of Medical Microecology(Putian University), Fujian Province University, School of Pharmacy and Medical Technology, Putian University, Putian 351100, Fujian Province, China.Key Laboratory of Medical Microecology(Putian University), Fujian Province University, School of Pharmacy and Medical Technology, Putian University, Putian 351100, Fujian Province, China.Key Laboratory of Medical Microecology(Putian University), Fujian Province University, School of Pharmacy and Medical Technology, Putian University, Putian 351100, Fujian Province, China.Key Laboratory of Medical Microecology(Putian University), Fujian Province University, School of Pharmacy and Medical Technology, Putian University, Putian 351100, Fujian Province, China.Key Laboratory of Medical Microecology(Putian University), Fujian Province University, School of Pharmacy and Medical Technology, Putian University, Putian 351100, Fujian Province, China.

Pub Type(s)

Comparative Study
Journal Article

Language

chi

PubMed ID

32762171

Citation

Lun, Yongzhi, et al. "[Comparative Analysis of Structural Characteristics and Epitopes in S Proteins Between SARS-CoV-2 and SARS-CoV]." Zhejiang Da Xue Xue Bao. Yi Xue Ban = Journal of Zhejiang University. Medical Sciences, vol. 49, no. 3, 2020, pp. 315-323.
Lun Y, Liu B, Dong W, et al. [Comparative analysis of structural characteristics and epitopes in S proteins between SARS-CoV-2 and SARS-CoV]. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2020;49(3):315-323.
Lun, Y., Liu, B., Dong, W., Sun, J., & Pan, L. (2020). [Comparative analysis of structural characteristics and epitopes in S proteins between SARS-CoV-2 and SARS-CoV]. Zhejiang Da Xue Xue Bao. Yi Xue Ban = Journal of Zhejiang University. Medical Sciences, 49(3), 315-323. https://doi.org/10.3785/j.issn.1008-9292.2020.06.06
Lun Y, et al. [Comparative Analysis of Structural Characteristics and Epitopes in S Proteins Between SARS-CoV-2 and SARS-CoV]. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2020 May 25;49(3):315-323. PubMed PMID: 32762171.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Comparative analysis of structural characteristics and epitopes in S proteins between SARS-CoV-2 and SARS-CoV]. AU - Lun,Yongzhi, AU - Liu,Ben, AU - Dong,Wen, AU - Sun,Jie, AU - Pan,Linghong, PY - 2020/8/8/entrez PY - 2020/8/8/pubmed PY - 2020/8/13/medline SP - 315 EP - 323 JF - Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences JO - Zhejiang Da Xue Xue Bao Yi Xue Ban VL - 49 IS - 3 N2 - OBJECTIVE: To provide data support for the study of pathogenic mechanism of SARS-CoV-2 at the molecular level, and provide suitable candidate targets for vaccine, antibody and drug research and development through comparative analysis for structural characteristics and epitopes of S protein of SARS-CoV-2 and SARS-CoV. METHODS: Based on the reference sequences of S protein, physical and chemical properties, hydrophobicity, signal peptide, transmembrane region, domain, secondary structure, tertiary structure analysis and antigenic epitopes prediction were carried out. Meanwhile, the tissue expression, related pathways and reactome pathways of angiotensis Ⅰ converting enzyme 2 (ACE2) and C-type lectin domain family 4 member M (CLEC4M) receptors were analyzed. RESULTS: The amino acid sequence of S protein of SARS-CoV-2 and SARS-CoV has a 75.80% consistency. The structural characteristics of the two coronaviruses are highly consistent, but the secondary structure and tertiary structure of SARS-CoV-2 is not as obvious as SARS-CoV. ACE2 and CLEC4M are expressed in alimentary system, heart, kidney, lung and placenta. The main related the pathways of renin-angiotensin system, protein digestion and absorption pathway, and the reactome pathways of metabolism of angiotensinogen to angiotensins, GPCR ligand binding, are related to typical symptoms of coronavirus disease 2019 induced by SARS-CoV-2. Three pairs of highly or completely homologous epitopes of S protein were obtained. The 600-605, 695-703 and 888-896 amino acid residues in SARS-CoV-2 were highly homologous with 586-591, 677-685 and 870-878 amino acid residues in SARS-CoV, respectively. CONCLUSIONS: The similarity of S protein of SARS-CoV-2 and SARS-CoV determines that they have similar infection patterns and clinical manifestations. The candidate epitopes with high reliability can provide reference for virus diagnosis and vaccine development. SN - 1008-9292 UR - https://www.unboundmedicine.com/medline/citation/32762171/[Comparative_analysis_of_structural_characteristics_and_epitopes_in_S_proteins_between_SARS-CoV-2_and_SARS-CoV] DB - PRIME DP - Unbound Medicine ER -