Tags

Type your tag names separated by a space and hit enter

Mouse liver dihydrodiol dehydrogenases. Identity of the predominant and a minor form with 17 beta-hydroxysteroid dehydrogenase and aldehyde reductase.
Biochem Pharmacol. 1988 Feb 01; 37(3):453-8.BP

Abstract

A major and a minor form of dihydrodiol dehydrogenase were co-purified with 17 beta-hydroxysteroid dehydrogenase and aldehyde reductase, respectively, to apparent homogeneity from liver cytosol of male ddY mice. The activities of dihydrodiol dehydrogenase and testosterone dehydrogenase or aldehyde reductase of the two enzyme forms comigrated electrophoretically. The major form of the enzyme oxidized 17 beta-hydroxysteroids and nonsteroidal alicyclic alcohols and reduced 17-ketosteroids and various synthetic carbonyl compounds, showing higher affinity for steroids than for xenobiotics. The activity of this enzyme form toward benzene dihydrodiol and testosterone exhibited identical thermostability and susceptibility to inhibition by quercitrin, SH-reagents, nonsteroidal estrogens and anti-inflammatory agents. On the other hand, the minor form of the enzyme, which oxidized benzene dihydrodiol but not 17 beta-hydroxysteroids, also reduced various aldehydes well and was specifically inhibited by barbiturates and sorbinil. These results indicate that the major form of dihydrodiol dehydrogenase is identical to 17 beta-hydroxysteroid dehydrogenase and the minor enzyme form to aldehyde reductase.

Authors+Show Affiliations

Department of Biochemistry, Gifu Pharmaceutical University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3276326

Citation

Sawada, H, et al. "Mouse Liver Dihydrodiol Dehydrogenases. Identity of the Predominant and a Minor Form With 17 Beta-hydroxysteroid Dehydrogenase and Aldehyde Reductase." Biochemical Pharmacology, vol. 37, no. 3, 1988, pp. 453-8.
Sawada H, Hara A, Nakayama T, et al. Mouse liver dihydrodiol dehydrogenases. Identity of the predominant and a minor form with 17 beta-hydroxysteroid dehydrogenase and aldehyde reductase. Biochem Pharmacol. 1988;37(3):453-8.
Sawada, H., Hara, A., Nakayama, T., Nakagawa, M., Inoue, Y., Hasebe, K., & Zhang, Y. P. (1988). Mouse liver dihydrodiol dehydrogenases. Identity of the predominant and a minor form with 17 beta-hydroxysteroid dehydrogenase and aldehyde reductase. Biochemical Pharmacology, 37(3), 453-8.
Sawada H, et al. Mouse Liver Dihydrodiol Dehydrogenases. Identity of the Predominant and a Minor Form With 17 Beta-hydroxysteroid Dehydrogenase and Aldehyde Reductase. Biochem Pharmacol. 1988 Feb 1;37(3):453-8. PubMed PMID: 3276326.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mouse liver dihydrodiol dehydrogenases. Identity of the predominant and a minor form with 17 beta-hydroxysteroid dehydrogenase and aldehyde reductase. AU - Sawada,H, AU - Hara,A, AU - Nakayama,T, AU - Nakagawa,M, AU - Inoue,Y, AU - Hasebe,K, AU - Zhang,Y P, PY - 1988/2/1/pubmed PY - 1988/2/1/medline PY - 1988/2/1/entrez SP - 453 EP - 8 JF - Biochemical pharmacology JO - Biochem. Pharmacol. VL - 37 IS - 3 N2 - A major and a minor form of dihydrodiol dehydrogenase were co-purified with 17 beta-hydroxysteroid dehydrogenase and aldehyde reductase, respectively, to apparent homogeneity from liver cytosol of male ddY mice. The activities of dihydrodiol dehydrogenase and testosterone dehydrogenase or aldehyde reductase of the two enzyme forms comigrated electrophoretically. The major form of the enzyme oxidized 17 beta-hydroxysteroids and nonsteroidal alicyclic alcohols and reduced 17-ketosteroids and various synthetic carbonyl compounds, showing higher affinity for steroids than for xenobiotics. The activity of this enzyme form toward benzene dihydrodiol and testosterone exhibited identical thermostability and susceptibility to inhibition by quercitrin, SH-reagents, nonsteroidal estrogens and anti-inflammatory agents. On the other hand, the minor form of the enzyme, which oxidized benzene dihydrodiol but not 17 beta-hydroxysteroids, also reduced various aldehydes well and was specifically inhibited by barbiturates and sorbinil. These results indicate that the major form of dihydrodiol dehydrogenase is identical to 17 beta-hydroxysteroid dehydrogenase and the minor enzyme form to aldehyde reductase. SN - 0006-2952 UR - https://www.unboundmedicine.com/medline/citation/3276326/Mouse_liver_dihydrodiol_dehydrogenases__Identity_of_the_predominant_and_a_minor_form_with_17_beta_hydroxysteroid_dehydrogenase_and_aldehyde_reductase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0006-2952(88)90214-6 DB - PRIME DP - Unbound Medicine ER -