Tags

Type your tag names separated by a space and hit enter

DNA nanoscaffold-based SARS-CoV-2 detection for COVID-19 diagnosis.
Biosens Bioelectron. 2020 Nov 01; 167:112479.BB

Abstract

COVID-19 pandemic outbreak is the most astounding scene ever experienced in the 21st century. It has been determined to be caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). With the global pandemic, the lack of efficient rapid and accurate molecular diagnostic testing tools has hindered the public opportunely response to the emerging viral threat. Herein, a DNA nanoscaffold hybrid chain reaction (DNHCR)-based nucleic acid assay strategy is reported for rapid detection of SARS-CoV-2 RNA. In this method, the DNA nanoscaffolds have been first constructed by the self-assembly of long DNA strands and self-quenching probes (H1). Then, the SARS-CoV-2 RNA will initiate the hybridization of H1 and free H2 DNA probes along the nanoscaffold, and an illuminated DNA nanostring is instantly obtained. By taking advantages of the localization design of the H1 probes and the temperature tolerance of the isothermal amplification, the proposed DNHCR method can detect target at short responding time (within 10 min) and mild condition (15 °C-35 °C). Moreover, the reliability of DNHCR method in serum and saliva samples have also been validated. Therefore, DNHCR-based method is expected to provide a simple and faster alternative to the traditional SARS-CoV-2 qRT-PCR assay.

Authors+Show Affiliations

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, PR China.State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, PR China.State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, PR China.State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, PR China.Department of Geriatric Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210023, PR China. Electronic address: swh@njmu.edu.cn.State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, PR China. Electronic address: xiangy@nju.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32763826

Citation

Jiao, Jin, et al. "DNA Nanoscaffold-based SARS-CoV-2 Detection for COVID-19 Diagnosis." Biosensors & Bioelectronics, vol. 167, 2020, p. 112479.
Jiao J, Duan C, Xue L, et al. DNA nanoscaffold-based SARS-CoV-2 detection for COVID-19 diagnosis. Biosens Bioelectron. 2020;167:112479.
Jiao, J., Duan, C., Xue, L., Liu, Y., Sun, W., & Xiang, Y. (2020). DNA nanoscaffold-based SARS-CoV-2 detection for COVID-19 diagnosis. Biosensors & Bioelectronics, 167, 112479. https://doi.org/10.1016/j.bios.2020.112479
Jiao J, et al. DNA Nanoscaffold-based SARS-CoV-2 Detection for COVID-19 Diagnosis. Biosens Bioelectron. 2020 Nov 1;167:112479. PubMed PMID: 32763826.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA nanoscaffold-based SARS-CoV-2 detection for COVID-19 diagnosis. AU - Jiao,Jin, AU - Duan,Chengjie, AU - Xue,Lan, AU - Liu,Yunfei, AU - Sun,Weihao, AU - Xiang,Yang, Y1 - 2020/07/29/ PY - 2020/07/12/received PY - 2020/07/24/revised PY - 2020/07/25/accepted PY - 2020/8/9/pubmed PY - 2020/9/18/medline PY - 2020/8/9/entrez KW - DNA nanoscaffold KW - DNA self-Assembly KW - Isothermal amplification KW - RNA detection KW - SARS-CoV-2 SP - 112479 EP - 112479 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 167 N2 - COVID-19 pandemic outbreak is the most astounding scene ever experienced in the 21st century. It has been determined to be caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). With the global pandemic, the lack of efficient rapid and accurate molecular diagnostic testing tools has hindered the public opportunely response to the emerging viral threat. Herein, a DNA nanoscaffold hybrid chain reaction (DNHCR)-based nucleic acid assay strategy is reported for rapid detection of SARS-CoV-2 RNA. In this method, the DNA nanoscaffolds have been first constructed by the self-assembly of long DNA strands and self-quenching probes (H1). Then, the SARS-CoV-2 RNA will initiate the hybridization of H1 and free H2 DNA probes along the nanoscaffold, and an illuminated DNA nanostring is instantly obtained. By taking advantages of the localization design of the H1 probes and the temperature tolerance of the isothermal amplification, the proposed DNHCR method can detect target at short responding time (within 10 min) and mild condition (15 °C-35 °C). Moreover, the reliability of DNHCR method in serum and saliva samples have also been validated. Therefore, DNHCR-based method is expected to provide a simple and faster alternative to the traditional SARS-CoV-2 qRT-PCR assay. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/32763826/DNA_nanoscaffold_based_SARS_CoV_2_detection_for_COVID_19_diagnosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(20)30472-3 DB - PRIME DP - Unbound Medicine ER -