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Comprehensive Analyses of SARS-CoV-2 Transmission in a Public Health Virology Laboratory.
Viruses. 2020 08 05; 12(8)V

Abstract

SARS-CoV-2 has become a major global concern as of December 2019, particularly affecting healthcare workers. As person-to-person transmission is airborne, crowded closed spaces have high potential for rapid virus spread, especially early in the pandemic when social distancing and mask wearing were not mandatory. This retrospective study thoroughly investigates a small-scale SARS-CoV-2 outbreak in Israel's central virology laboratory (ICVL) in mid-March 2020, in which six staff members and two related family members were infected. Suspicions regarding infection by contaminated surfaces in ICVL facilities were nullified by SARS-CoV-2 negative real time polymerase chain reaction (PCR) of work surfaces swipe tests. Complete SARS-CoV-2 genomes were sequenced and mutation analyses showed inclusion of all samples to clades 20B and 20C, possessing the spike mutation D614G. Phylogenetic analysis clarified transmission events, confirming S1 as having infected at least three other staff members and refuting the association of a staff member's infected spouse with the ICVL transmission cluster. Finally, serology tests exhibited IgG and IgA antibodies in all infected individuals and revealed the occurrence of asymptomatic infections in additional staff members. This study demonstrates the advantages of molecular epidemiology in elucidating transmission events and exemplifies the importance of good laboratory practice, distancing and mask wearing in preventing SARS-CoV-2 spread, specifically in healthcare facilities.

Authors+Show Affiliations

Central Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, P.O. Box 5265601, Ramat-Gan, Israel.Central Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, P.O. Box 5265601, Ramat-Gan, Israel. The Israel Center for Disease Control, Israel Ministry of Health, P.O. Box 5265601, Tel-Hashomer, Israel.Central Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, P.O. Box 5265601, Ramat-Gan, Israel.Central Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, P.O. Box 5265601, Ramat-Gan, Israel. Department of Epidemiology and Preventive Medicine, School of Public Health, Sackler Faculty of Medicine, Tel-Aviv University, P.O. Box 39040, Tel-Aviv, Israel.Central Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, P.O. Box 5265601, Ramat-Gan, Israel.Central Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, P.O. Box 5265601, Ramat-Gan, Israel.Central Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, P.O. Box 5265601, Ramat-Gan, Israel. Department of Epidemiology and Preventive Medicine, School of Public Health, Sackler Faculty of Medicine, Tel-Aviv University, P.O. Box 39040, Tel-Aviv, Israel.Central Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, P.O. Box 5265601, Ramat-Gan, Israel. Department of Epidemiology and Preventive Medicine, School of Public Health, Sackler Faculty of Medicine, Tel-Aviv University, P.O. Box 39040, Tel-Aviv, Israel.Central Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, P.O. Box 5265601, Ramat-Gan, Israel. Department of Epidemiology and Preventive Medicine, School of Public Health, Sackler Faculty of Medicine, Tel-Aviv University, P.O. Box 39040, Tel-Aviv, Israel.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32764372

Citation

Zuckerman, Neta S., et al. "Comprehensive Analyses of SARS-CoV-2 Transmission in a Public Health Virology Laboratory." Viruses, vol. 12, no. 8, 2020.
Zuckerman NS, Pando R, Bucris E, et al. Comprehensive Analyses of SARS-CoV-2 Transmission in a Public Health Virology Laboratory. Viruses. 2020;12(8).
Zuckerman, N. S., Pando, R., Bucris, E., Drori, Y., Lustig, Y., Erster, O., Mor, O., Mendelson, E., & Mandelboim, M. (2020). Comprehensive Analyses of SARS-CoV-2 Transmission in a Public Health Virology Laboratory. Viruses, 12(8). https://doi.org/10.3390/v12080854
Zuckerman NS, et al. Comprehensive Analyses of SARS-CoV-2 Transmission in a Public Health Virology Laboratory. Viruses. 2020 08 5;12(8) PubMed PMID: 32764372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comprehensive Analyses of SARS-CoV-2 Transmission in a Public Health Virology Laboratory. AU - Zuckerman,Neta S, AU - Pando,Rakefet, AU - Bucris,Efrat, AU - Drori,Yaron, AU - Lustig,Yaniv, AU - Erster,Oran, AU - Mor,Orna, AU - Mendelson,Ella, AU - Mandelboim,Michal, Y1 - 2020/08/05/ PY - 2020/07/09/received PY - 2020/07/29/revised PY - 2020/07/31/accepted PY - 2020/8/9/entrez PY - 2020/8/9/pubmed PY - 2020/9/10/medline KW - 2019-nCoV KW - COVID-19 KW - SARS-CoV-2 KW - infection KW - next generation sequencing (NGS) KW - staff JF - Viruses JO - Viruses VL - 12 IS - 8 N2 - SARS-CoV-2 has become a major global concern as of December 2019, particularly affecting healthcare workers. As person-to-person transmission is airborne, crowded closed spaces have high potential for rapid virus spread, especially early in the pandemic when social distancing and mask wearing were not mandatory. This retrospective study thoroughly investigates a small-scale SARS-CoV-2 outbreak in Israel's central virology laboratory (ICVL) in mid-March 2020, in which six staff members and two related family members were infected. Suspicions regarding infection by contaminated surfaces in ICVL facilities were nullified by SARS-CoV-2 negative real time polymerase chain reaction (PCR) of work surfaces swipe tests. Complete SARS-CoV-2 genomes were sequenced and mutation analyses showed inclusion of all samples to clades 20B and 20C, possessing the spike mutation D614G. Phylogenetic analysis clarified transmission events, confirming S1 as having infected at least three other staff members and refuting the association of a staff member's infected spouse with the ICVL transmission cluster. Finally, serology tests exhibited IgG and IgA antibodies in all infected individuals and revealed the occurrence of asymptomatic infections in additional staff members. This study demonstrates the advantages of molecular epidemiology in elucidating transmission events and exemplifies the importance of good laboratory practice, distancing and mask wearing in preventing SARS-CoV-2 spread, specifically in healthcare facilities. SN - 1999-4915 UR - https://www.unboundmedicine.com/medline/citation/32764372/Comprehensive_Analyses_of_SARS_CoV_2_Transmission_in_a_Public_Health_Virology_Laboratory_ L2 - https://www.mdpi.com/resolver?pii=v12080854 DB - PRIME DP - Unbound Medicine ER -