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Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells.
Nat Commun. 2020 08 06; 11(1):3910.NC

Abstract

SARS-CoV-2, a β-coronavirus, has rapidly spread across the world, highlighting its high transmissibility, but the underlying morphogenesis and pathogenesis remain poorly understood. Here, we characterize the replication dynamics, cell tropism and morphogenesis of SARS-CoV-2 in organotypic human airway epithelial (HAE) cultures. SARS-CoV-2 replicates efficiently and infects both ciliated and secretory cells in HAE cultures. In comparison, HCoV-NL63 replicates to lower titers and is only detected in ciliated cells. SARS-CoV-2 shows a similar morphogenetic process as other coronaviruses but causes plaque-like cytopathic effects in HAE cultures. Cell fusion, apoptosis, destruction of epithelium integrity, cilium shrinking and beaded changes are observed in the plaque regions. Taken together, our results provide important insights into SARS-CoV-2 cell tropism, replication and morphogenesis.

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Authors+Show Affiliations

Zhu N
NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
Wang W
NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
Liu Z
Department of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University (Beijing Tuberculosis and Thoracic Tumor Research Institute), 101149, Beijing, China.
Liang C
Department of Thoracic Surgery, China-Japan Friendship Hospital, Yinghua East Road No. 2, Chaoyang District, 100029, Beijing, China.
Wang W
NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
Ye F
NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
Huang B
NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
Zhao L
NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
Wang H
NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
Zhou W
NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
Deng Y
NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
Mao L
Suzhou Institute of Systems Medicine, 215123, Suzhou, Jiangsu, China.
Su C
Department of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University (Beijing Tuberculosis and Thoracic Tumor Research Institute), 101149, Beijing, China.
Qiang G
Department of Thoracic Surgery, China-Japan Friendship Hospital, Yinghua East Road No. 2, Chaoyang District, 100029, Beijing, China.
Jiang T
Suzhou Institute of Systems Medicine, 215123, Suzhou, Jiangsu, China.
Zhao J
State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, 510120, Guangzhou, China. Institute of Infectious Disease, Guangzhou Eighth People's Hospital of Guangzhou Medical University, 510120, Guangzhou, China.
Wu G
NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
Song J
NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China. songjd@ivdc.chinacdc.cn. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China. songjd@ivdc.chinacdc.cn.
Tan W
NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China. tanwj@ivdc.chinacdc.cn. Center for Biosafety Mega-Science, Chinese Academy of Sciences, 430071, Wuhan, China. tanwj@ivdc.chinacdc.cn.

MeSH

BetacoronavirusCOVID-19Cell LineCells, CulturedCoronavirus InfectionsCytopathogenic Effect, ViralEpithelial CellsHumansMorphogenesisPandemicsPneumonia, ViralRespiratory SystemSARS-CoV-2TropismVirus Replication

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32764693

Citation

Zhu, Na, et al. "Morphogenesis and Cytopathic Effect of SARS-CoV-2 Infection in Human Airway Epithelial Cells." Nature Communications, vol. 11, no. 1, 2020, p. 3910.
Zhu N, Wang W, Liu Z, et al. Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells. Nat Commun. 2020;11(1):3910.
Zhu, N., Wang, W., Liu, Z., Liang, C., Wang, W., Ye, F., Huang, B., Zhao, L., Wang, H., Zhou, W., Deng, Y., Mao, L., Su, C., Qiang, G., Jiang, T., Zhao, J., Wu, G., Song, J., & Tan, W. (2020). Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells. Nature Communications, 11(1), 3910. https://doi.org/10.1038/s41467-020-17796-z
Zhu N, et al. Morphogenesis and Cytopathic Effect of SARS-CoV-2 Infection in Human Airway Epithelial Cells. Nat Commun. 2020 08 6;11(1):3910. PubMed PMID: 32764693.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells. AU - Zhu,Na, AU - Wang,Wenling, AU - Liu,Zhidong, AU - Liang,Chaoyang, AU - Wang,Wen, AU - Ye,Fei, AU - Huang,Baoying, AU - Zhao,Li, AU - Wang,Huijuan, AU - Zhou,Weimin, AU - Deng,Yao, AU - Mao,Longfei, AU - Su,Chongyu, AU - Qiang,Guangliang, AU - Jiang,Taijiao, AU - Zhao,Jincun, AU - Wu,Guizhen, AU - Song,Jingdong, AU - Tan,Wenjie, Y1 - 2020/08/06/ PY - 2020/05/25/received PY - 2020/07/15/accepted PY - 2020/8/9/entrez PY - 2020/8/9/pubmed PY - 2020/8/26/medline SP - 3910 EP - 3910 JF - Nature communications JO - Nat Commun VL - 11 IS - 1 N2 - SARS-CoV-2, a β-coronavirus, has rapidly spread across the world, highlighting its high transmissibility, but the underlying morphogenesis and pathogenesis remain poorly understood. Here, we characterize the replication dynamics, cell tropism and morphogenesis of SARS-CoV-2 in organotypic human airway epithelial (HAE) cultures. SARS-CoV-2 replicates efficiently and infects both ciliated and secretory cells in HAE cultures. In comparison, HCoV-NL63 replicates to lower titers and is only detected in ciliated cells. SARS-CoV-2 shows a similar morphogenetic process as other coronaviruses but causes plaque-like cytopathic effects in HAE cultures. Cell fusion, apoptosis, destruction of epithelium integrity, cilium shrinking and beaded changes are observed in the plaque regions. Taken together, our results provide important insights into SARS-CoV-2 cell tropism, replication and morphogenesis. SN - 2041-1723 UR - https://www.unboundmedicine.com/medline/citation/32764693/Morphogenesis_and_cytopathic_effect_of_SARS_CoV_2_infection_in_human_airway_epithelial_cells_ DB - PRIME DP - Unbound Medicine ER -