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Prevalence and genotype/subtype distribution of hepatitis E virus (HEV) among wild boars in Japan: Identification of a genotype 5 HEV strain.
Virus Res. 2020 10 02; 287:198106.VR

Abstract

To further investigate the prevalence of hepatitis E virus (HEV) infection and characterize HEV genomes among Japanese wild boars (Sus scrofa leucomystax), 1880 boars captured in 17 prefectures in Japan from 2013 to 2019 were studied. Overall, anti-HEV IgG was detected in 8.9 % and HEV RNA was detected in 3.9 % of boars, which was comparable with our previous studies during 2003-2013 (10.3 % and 3.5 %, respectively). Among 74 boar HEV strains obtained from infected boars in the present study, 50 (68 %) were classified into genotype 3 (3a and 3b), 23 (31 %) were classified into genotype 4 (4i), and the remaining strain (wbJGF_19-1) was classified into genotype 5. The wbGF_19-1 strain shared 92.7 % identity over the entire genome with the prototype genotype 5 strain (JBOAR135-Shiz09). The identification of the second genotype 5 HEV strain in a place that is located only 100 km from the site at which JBOAR135-Shiz09 was identified, suggests that genotype 5 HEV circulates within a relatively close range in Japan. Genetically similar HEV strains forming a clade were identified from wild boars living in each area during the observation period of 11-13 years, although the nucleotide sequence changed gradually, accounting for up to 3.4-3.6 % within the 412-nucleotide ORF2 sequence. Eight groups of boars with a cluster of HEV infections were observed, consisting of two, three or four infected offspring, presumably born to the same mother or offspring with their mother. These results suggest that wild boars continue to be important reservoirs for HEV infection in humans in Japan.

Authors+Show Affiliations

Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke, Tochigi-Ken 329-0498, Japan.Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke, Tochigi-Ken 329-0498, Japan.Department of Internal Medicine, Kamiichi General Hospital, Nakaniikawa-Gun, Toyama-Ken 930-0391, Japan.Department of Gastroenterology, Tottori Seikyo Hospital, Tottori, Tottori-Ken 680-0833, Japan.Aikawa Internal Medicine Hospital, Mito, Ibaraki-Ken 310-0851, Japan.Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Okayama-Ken 700-8558, Japan.Seiyo Municipal Hospital, Seiyo, Ehime-Ken 797-0029, Japan.Seiyo Municipal Hospital, Seiyo, Ehime-Ken 797-0029, Japan.Toei Medical Center, Kitashitara-Gun, Aichi-Ken 449-0216, Japan.Iida Public Health Center, Iida, Nagano-Ken 395-0034, Japan.Ozaki Homecare Clinic, Yufu, Oita-Ken 879-5434, Japan.Center for Weeds and Wildlife Management, Utsunomiya University, Utsunomiya, Tochigi-Ken 321-8505, Japan.Irokawa Clinic, Hanishina-Gun, Nagano-Ken 389-0601, Japan.National Health Insurance Shirotori Hospital, Gujo, Gifu-Ken 501-5122, Japan.Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke, Tochigi-Ken 329-0498, Japan.Center of Liver Disease, Okayama Saiseikai General Hospital, Okayama, Okayama-Ken 700-8511, Japan.Department of Internal Medicine, Kin-ikyo Chuo Hospital, Sapporo, Hokkaido 007-8505, Japan.Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke, Tochigi-Ken 329-0498, Japan. Electronic address: hokamoto@jichi.ac.jp.Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke, Tochigi-Ken 329-0498, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32777387

Citation

Takahashi, Masaharu, et al. "Prevalence and Genotype/subtype Distribution of Hepatitis E Virus (HEV) Among Wild Boars in Japan: Identification of a Genotype 5 HEV Strain." Virus Research, vol. 287, 2020, p. 198106.
Takahashi M, Nishizawa T, Sato Y, et al. Prevalence and genotype/subtype distribution of hepatitis E virus (HEV) among wild boars in Japan: Identification of a genotype 5 HEV strain. Virus Res. 2020;287:198106.
Takahashi, M., Nishizawa, T., Sato, Y., Miyazaki, S., Aikawa, T., Ashida, K., Tamaru, T., Oguro, K., Hayakawa, F., Matsuoka, H., Ozaki, H., Kodera, Y., Irokawa, M., Hirose, H., Nagashima, S., Kawakami, M., Mizuo, H., Okamoto, H., & Murata, K. (2020). Prevalence and genotype/subtype distribution of hepatitis E virus (HEV) among wild boars in Japan: Identification of a genotype 5 HEV strain. Virus Research, 287, 198106. https://doi.org/10.1016/j.virusres.2020.198106
Takahashi M, et al. Prevalence and Genotype/subtype Distribution of Hepatitis E Virus (HEV) Among Wild Boars in Japan: Identification of a Genotype 5 HEV Strain. Virus Res. 2020 10 2;287:198106. PubMed PMID: 32777387.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence and genotype/subtype distribution of hepatitis E virus (HEV) among wild boars in Japan: Identification of a genotype 5 HEV strain. AU - Takahashi,Masaharu, AU - Nishizawa,Tsutomu, AU - Sato,Yukihiro, AU - Miyazaki,Shinichi, AU - Aikawa,Tatsuya, AU - Ashida,Kozo, AU - Tamaru,Tomoko, AU - Oguro,Kunihiko, AU - Hayakawa,Fumihiro, AU - Matsuoka,Hiroyuki, AU - Ozaki,Hideaki, AU - Kodera,Yuuji, AU - Irokawa,Masahiko, AU - Hirose,Hideo, AU - Nagashima,Shigeo, AU - Kawakami,Manri, AU - Mizuo,Hitoshi, AU - Okamoto,Hiroaki, AU - Murata,Kazumoto, Y1 - 2020/08/07/ PY - 2020/06/15/received PY - 2020/07/28/revised PY - 2020/07/29/accepted PY - 2020/8/11/pubmed PY - 2021/9/10/medline PY - 2020/8/11/entrez KW - Cluster infection KW - Genotype 5 KW - Hepatitis E virus KW - Wild boar SP - 198106 EP - 198106 JF - Virus research JO - Virus Res VL - 287 N2 - To further investigate the prevalence of hepatitis E virus (HEV) infection and characterize HEV genomes among Japanese wild boars (Sus scrofa leucomystax), 1880 boars captured in 17 prefectures in Japan from 2013 to 2019 were studied. Overall, anti-HEV IgG was detected in 8.9 % and HEV RNA was detected in 3.9 % of boars, which was comparable with our previous studies during 2003-2013 (10.3 % and 3.5 %, respectively). Among 74 boar HEV strains obtained from infected boars in the present study, 50 (68 %) were classified into genotype 3 (3a and 3b), 23 (31 %) were classified into genotype 4 (4i), and the remaining strain (wbJGF_19-1) was classified into genotype 5. The wbGF_19-1 strain shared 92.7 % identity over the entire genome with the prototype genotype 5 strain (JBOAR135-Shiz09). The identification of the second genotype 5 HEV strain in a place that is located only 100 km from the site at which JBOAR135-Shiz09 was identified, suggests that genotype 5 HEV circulates within a relatively close range in Japan. Genetically similar HEV strains forming a clade were identified from wild boars living in each area during the observation period of 11-13 years, although the nucleotide sequence changed gradually, accounting for up to 3.4-3.6 % within the 412-nucleotide ORF2 sequence. Eight groups of boars with a cluster of HEV infections were observed, consisting of two, three or four infected offspring, presumably born to the same mother or offspring with their mother. These results suggest that wild boars continue to be important reservoirs for HEV infection in humans in Japan. SN - 1872-7492 UR - https://www.unboundmedicine.com/medline/citation/32777387/Prevalence_and_genotype/subtype_distribution_of_hepatitis_E_virus__HEV__among_wild_boars_in_Japan:_Identification_of_a_genotype_5_HEV_strain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1702(20)31013-3 DB - PRIME DP - Unbound Medicine ER -