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The characterization of extracellular vesicles-derived microRNAs in Thai malaria patients.
Malar J. 2020 Aug 10; 19(1):285.MJ

Abstract

BACKGROUND

Extracellular vesicles (EVs) have been broadly studied in malaria for nearly a decade. These vesicles carry various functional biomolecules including RNA families such as microRNAs (miRNA). These EVs-derived microRNAs have numerous roles in host-parasite interactions and are considered promising biomarkers for disease severity. However, this field lacks clinical studies of malaria-infected samples. In this study, EV specific miRNAs were isolated from the plasma of patients from Thailand infected with Plasmodium vivax and Plasmodium falciparum. In addition, it is postulated that these miRNAs were differentially expressed in these groups of patients and had a role in disease onset through the regulation of specific target genes.

METHODS

EVs were purified from the plasma of Thai P. vivax-infected patients (n = 19), P. falciparum-infected patients (n = 18) and uninfected individuals (n = 20). EV-derived miRNAs were then prepared and abundance of hsa-miR-15b-5p, hsa-miR-16-5p, hsa-let-7a-5p and hsa-miR-150-5p was assessed in these samples. Quantitative polymerase chain reaction was performed, and relative expression of each miRNA was calculated using hsa-miR-451a as endogenous control. Then, the targets of up-regulated miRNAs and relevant pathways were predicted by using bioinformatics. Receiver Operating Characteristic with Area under the Curve (AUC) was then calculated to assess their diagnostic potential.

RESULTS

The relative expression of hsa-miR-150-5p and hsa-miR-15b-5p was higher in P. vivax-infected patients compared to uninfected individuals, but hsa-let-7a-5p was up-regulated in both P. vivax-infected patients and P. falciparum-infected patients. Bioinformatic analysis revealed that these miRNAs might regulate genes involved in the malaria pathway including the adherens junction and the transforming growth factor-β pathways. All up-regulated miRNAs could potentially be used as disease biomarkers as determined by AUC; however, the sensitivity and specificity require further investigation.

CONCLUSION

An upregulation of hsa-miR-150-5p and hsa-miR-15b-5p was observed in P. vivax-infected patients while hsa-let-7a-5p was up-regulated in both P. vivax-infected and P. falciparum-infected patients. These findings will require further validation in larger cohort groups of malaria patients to fully understand the contribution of these EVs miRNAs to malaria detection and biology.

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Authors+Show Affiliations

Ketprasit N
Graduate Programme in Clinical Hematology Sciences, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand. Malaria and Microvesicles Research Group, School of Life Sciences, Faculty of Sciences, University Technology of Sydney, Ultimo, Sydney, NSW, 2007, Australia.
Cheng IS
Malaria and Microvesicles Research Group, School of Life Sciences, Faculty of Sciences, University Technology of Sydney, Ultimo, Sydney, NSW, 2007, Australia.
Deutsch F
Non-coding RNA Cancer Group, School of Biomedical Engineering, Faculty of Engineering and IT, University Technology of Sydney, Sydney, NSW, Australia.
Tran N
Non-coding RNA Cancer Group, School of Biomedical Engineering, Faculty of Engineering and IT, University Technology of Sydney, Sydney, NSW, Australia.
Imwong M
Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Combes V
Malaria and Microvesicles Research Group, School of Life Sciences, Faculty of Sciences, University Technology of Sydney, Ultimo, Sydney, NSW, 2007, Australia. valery.combes@uts.edu.au.
Palasuwan D
Oxidation in Red Cell Disorders Research Unit, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, 154 Rama 1 Road, Pathumwan, Bangkok, 10330, Thailand. nantadao@gmail.com.

MeSH

AdultExtracellular VesiclesFemaleHumansMalaria, FalciparumMalaria, VivaxMaleMicroRNAsMiddle AgedPlasmodium falciparumPlasmodium vivaxThailandYoung Adult

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32778117

Citation

Ketprasit, Nutpakal, et al. "The Characterization of Extracellular Vesicles-derived microRNAs in Thai Malaria Patients." Malaria Journal, vol. 19, no. 1, 2020, p. 285.
Ketprasit N, Cheng IS, Deutsch F, et al. The characterization of extracellular vesicles-derived microRNAs in Thai malaria patients. Malar J. 2020;19(1):285.
Ketprasit, N., Cheng, I. S., Deutsch, F., Tran, N., Imwong, M., Combes, V., & Palasuwan, D. (2020). The characterization of extracellular vesicles-derived microRNAs in Thai malaria patients. Malaria Journal, 19(1), 285. https://doi.org/10.1186/s12936-020-03360-z
Ketprasit N, et al. The Characterization of Extracellular Vesicles-derived microRNAs in Thai Malaria Patients. Malar J. 2020 Aug 10;19(1):285. PubMed PMID: 32778117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The characterization of extracellular vesicles-derived microRNAs in Thai malaria patients. AU - Ketprasit,Nutpakal, AU - Cheng,Iris Simone, AU - Deutsch,Fiona, AU - Tran,Nham, AU - Imwong,Mallika, AU - Combes,Valery, AU - Palasuwan,Duangdao, Y1 - 2020/08/10/ PY - 2020/03/21/received PY - 2020/08/05/accepted PY - 2020/8/12/entrez PY - 2020/8/12/pubmed PY - 2021/3/11/medline KW - Extracellular vesicles KW - Malaria KW - Patients KW - Plasmodium falciparum KW - Plasmodium vivax KW - microRNAs SP - 285 EP - 285 JF - Malaria journal JO - Malar J VL - 19 IS - 1 N2 - BACKGROUND: Extracellular vesicles (EVs) have been broadly studied in malaria for nearly a decade. These vesicles carry various functional biomolecules including RNA families such as microRNAs (miRNA). These EVs-derived microRNAs have numerous roles in host-parasite interactions and are considered promising biomarkers for disease severity. However, this field lacks clinical studies of malaria-infected samples. In this study, EV specific miRNAs were isolated from the plasma of patients from Thailand infected with Plasmodium vivax and Plasmodium falciparum. In addition, it is postulated that these miRNAs were differentially expressed in these groups of patients and had a role in disease onset through the regulation of specific target genes. METHODS: EVs were purified from the plasma of Thai P. vivax-infected patients (n = 19), P. falciparum-infected patients (n = 18) and uninfected individuals (n = 20). EV-derived miRNAs were then prepared and abundance of hsa-miR-15b-5p, hsa-miR-16-5p, hsa-let-7a-5p and hsa-miR-150-5p was assessed in these samples. Quantitative polymerase chain reaction was performed, and relative expression of each miRNA was calculated using hsa-miR-451a as endogenous control. Then, the targets of up-regulated miRNAs and relevant pathways were predicted by using bioinformatics. Receiver Operating Characteristic with Area under the Curve (AUC) was then calculated to assess their diagnostic potential. RESULTS: The relative expression of hsa-miR-150-5p and hsa-miR-15b-5p was higher in P. vivax-infected patients compared to uninfected individuals, but hsa-let-7a-5p was up-regulated in both P. vivax-infected patients and P. falciparum-infected patients. Bioinformatic analysis revealed that these miRNAs might regulate genes involved in the malaria pathway including the adherens junction and the transforming growth factor-β pathways. All up-regulated miRNAs could potentially be used as disease biomarkers as determined by AUC; however, the sensitivity and specificity require further investigation. CONCLUSION: An upregulation of hsa-miR-150-5p and hsa-miR-15b-5p was observed in P. vivax-infected patients while hsa-let-7a-5p was up-regulated in both P. vivax-infected and P. falciparum-infected patients. These findings will require further validation in larger cohort groups of malaria patients to fully understand the contribution of these EVs miRNAs to malaria detection and biology. SN - 1475-2875 UR - https://www.unboundmedicine.com/medline/citation/32778117/The_characterization_of_extracellular_vesicles_derived_microRNAs_in_Thai_malaria_patients_ DB - PRIME DP - Unbound Medicine ER -