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Comparative Analysis of Primer-Probe Sets for RT-qPCR of COVID-19 Causative Virus (SARS-CoV-2).
ACS Infect Dis. 2020 09 11; 6(9):2513-2523.AI

Abstract

Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2, also previously known as 2019-nCoV). Within 8 months of the outbreak, more than 10,000,000 cases of COVID-19 have been confirmed worldwide. Since human-to-human transmission occurs easily and the rate of human infection is rapidly increasing, sensitive and early diagnosis is essential to prevent a global outbreak. Recently, the World Health Organization (WHO) announced various primer-probe sets for SARS-CoV-2 developed at different institutions: China Center for Disease Control and Prevention (China CDC, China), Charité (Germany), The University of Hong Kong (HKU, Hong Kong), National Institute of Infectious Diseases in Japan (Japan NIID, Japan), National Institute of Health in Thailand (Thailand NIH, Thailand), and US CDC (USA). In this study, we compared the ability to detect SARS-CoV-2 RNA among seven primer-probe sets for the N gene and three primer-probe sets for the Orf1 gene. The results revealed that "NIID_2019-nCOV_N" from the Japan NIID and "ORF1ab" from China CDC represent a recommendable performance of RT-qPCR analysis for SARS-CoV-2 molecular diagnostics without nonspecific amplification and cross-reactivity for hCoV-229E, hCoV-OC43, and MERS-CoV RNA. Therefore, the appropriate combination of NIID_2019-nCOV_N (Japan NIID) and ORF1ab (China CDC) sets should be selected for sensitive and reliable SARS-CoV-2 molecular diagnostics.

Authors+Show Affiliations

Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea. Research Group of Food Processing, Korea Food Research Institute, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.Department of Molecular Diagnostics, WELLS BIO, Inc., MagokJungang 8-ro 1-gil, Gangseo-gu, Seoul 07795, Republic of Korea.Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea. Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon 34114, Republic of Korea.Department of Internal Medicine, Jeonbuk National University Medical School, Jeonju, Jeollabuk-do 54986, Republic of Korea. Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju, Jeollabuk-do 54907, Republic of Korea.Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea. Division of Chemical and Medical Metrology, Center for Bioanalysis, Korea Research Institute of Standards and Science, Daejeon 34113, Republic of Korea.Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea. Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju, Chungcheongbuk-do 28119, Republic of Korea.Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea. Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon 34114, Republic of Korea.Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.Department of Molecular Diagnostics, WELLS BIO, Inc., MagokJungang 8-ro 1-gil, Gangseo-gu, Seoul 07795, Republic of Korea.Department of Molecular Diagnostics, WELLS BIO, Inc., MagokJungang 8-ro 1-gil, Gangseo-gu, Seoul 07795, Republic of Korea.Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea. Research Group of Food Processing, Korea Food Research Institute, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea. Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju, Chungcheongbuk-do 28119, Republic of Korea.Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.Department of Molecular Diagnostics, WELLS BIO, Inc., MagokJungang 8-ro 1-gil, Gangseo-gu, Seoul 07795, Republic of Korea.Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32786273

Citation

Jung, Yujin, et al. "Comparative Analysis of Primer-Probe Sets for RT-qPCR of COVID-19 Causative Virus (SARS-CoV-2)." ACS Infectious Diseases, vol. 6, no. 9, 2020, pp. 2513-2523.
Jung Y, Park GS, Moon JH, et al. Comparative Analysis of Primer-Probe Sets for RT-qPCR of COVID-19 Causative Virus (SARS-CoV-2). ACS Infect Dis. 2020;6(9):2513-2523.
Jung, Y., Park, G. S., Moon, J. H., Ku, K., Beak, S. H., Lee, C. S., Kim, S., Park, E. C., Park, D., Lee, J. H., Byeon, C. W., Lee, J. J., Maeng, J. S., Kim, S. J., Kim, S. I., Kim, B. T., Lee, M. J., & Kim, H. G. (2020). Comparative Analysis of Primer-Probe Sets for RT-qPCR of COVID-19 Causative Virus (SARS-CoV-2). ACS Infectious Diseases, 6(9), 2513-2523. https://doi.org/10.1021/acsinfecdis.0c00464
Jung Y, et al. Comparative Analysis of Primer-Probe Sets for RT-qPCR of COVID-19 Causative Virus (SARS-CoV-2). ACS Infect Dis. 2020 09 11;6(9):2513-2523. PubMed PMID: 32786273.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative Analysis of Primer-Probe Sets for RT-qPCR of COVID-19 Causative Virus (SARS-CoV-2). AU - Jung,Yujin, AU - Park,Gun-Soo, AU - Moon,Jun Hye, AU - Ku,Keunbon, AU - Beak,Seung-Hwa, AU - Lee,Chang-Seop, AU - Kim,Seil, AU - Park,Edmond Changkyun, AU - Park,Daeui, AU - Lee,Jong-Hwan, AU - Byeon,Cheol Woo, AU - Lee,Joong Jin, AU - Maeng,Jin-Soo, AU - Kim,Seong-Jun, AU - Kim,Seung Il, AU - Kim,Bum-Tae, AU - Lee,Min Jun, AU - Kim,Hong Gi, Y1 - 2020/08/29/ PY - 2020/8/14/pubmed PY - 2020/9/24/medline PY - 2020/8/14/entrez KW - 2019-nCoV KW - COVID-19 KW - SARS-CoV-2 KW - molecular diagnosis KW - real-time qPCR SP - 2513 EP - 2523 JF - ACS infectious diseases JO - ACS Infect Dis VL - 6 IS - 9 N2 - Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2, also previously known as 2019-nCoV). Within 8 months of the outbreak, more than 10,000,000 cases of COVID-19 have been confirmed worldwide. Since human-to-human transmission occurs easily and the rate of human infection is rapidly increasing, sensitive and early diagnosis is essential to prevent a global outbreak. Recently, the World Health Organization (WHO) announced various primer-probe sets for SARS-CoV-2 developed at different institutions: China Center for Disease Control and Prevention (China CDC, China), Charité (Germany), The University of Hong Kong (HKU, Hong Kong), National Institute of Infectious Diseases in Japan (Japan NIID, Japan), National Institute of Health in Thailand (Thailand NIH, Thailand), and US CDC (USA). In this study, we compared the ability to detect SARS-CoV-2 RNA among seven primer-probe sets for the N gene and three primer-probe sets for the Orf1 gene. The results revealed that "NIID_2019-nCOV_N" from the Japan NIID and "ORF1ab" from China CDC represent a recommendable performance of RT-qPCR analysis for SARS-CoV-2 molecular diagnostics without nonspecific amplification and cross-reactivity for hCoV-229E, hCoV-OC43, and MERS-CoV RNA. Therefore, the appropriate combination of NIID_2019-nCOV_N (Japan NIID) and ORF1ab (China CDC) sets should be selected for sensitive and reliable SARS-CoV-2 molecular diagnostics. SN - 2373-8227 UR - https://www.unboundmedicine.com/medline/citation/32786273/Comparative_Analysis_of_Primer_Probe_Sets_for_RT_qPCR_of_COVID_19_Causative_Virus__SARS_CoV_2__ L2 - https://doi.org/10.1021/acsinfecdis.0c00464 DB - PRIME DP - Unbound Medicine ER -