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Baseline Susceptibility of a Laboratory Strain of Northern Corn Rootworm, Diabrotica barberi (Coleoptera: Chrysomelidae) to Bacillus thuringiensis Traits in Seedling, Single Plant, and Diet-Toxicity Assays.
J Econ Entomol. 2020 08 13; 113(4):1955-1962.JE

Abstract

The northern corn rootworm (NCR), Diabrotica barberi Smith & Lawrence, is an economic pest of maize in the U.S. Corn Belt. The objective of this study was to determine the baseline susceptibility of a laboratory NCR strain to Bt proteins eCry3.1Ab, mCry3A, Cry3Bb1, and Cry34/35Ab1 using seedling, single plant, and diet-toxicity assays. Plant assays were performed in greenhouse using corn hybrids expressing one of the Bt proteins and each respective near-isoline. Diet-toxicity assays, consisting of Bt proteins overlaid onto artificial diet were also conducted. In both plant assays, significantly more larvae survived Cry34/35Ab1-expressing corn compared with all other Bt-expressing corn, and larvae that survived eCry3.1Ab-expressing corn had significantly smaller head capsule widths compared with larvae that survived Cry34/35Ab1-expressing corn. In seedling assays, larvae surviving eCry3.1Ab-expressing corn also had significantly smaller head capsule widths compared with larvae that survived mCry3A-expressing corn. Additionally, larvae that survived mCry3A-expressing corn weighed significantly more than larvae surviving eCry3.1Ab- and Cry34/35Ab1-expressing corn. In single plant assays, no significant differences in larval dry weight was observed between any of the Bt-expressing corn. In diet assays, LC50s ranged from 0.14 (eCry3.1Ab) to 10.6 µg/cm2 (Cry34/35Ab1), EC50s ranged from 0.12 (Cry34/35Ab1) to 1.57 µg/cm2 (mCry3A), IC50s ranged from 0.08 (eCry3.1Ab) to 2.41 µg/cm2 (Cry34/35Ab1), and MIC50s ranged from 2.52 (eCry3.1Ab) to 14.2 µg/cm2 (mCry3A). These results establish the toxicity of four Bt proteins to a laboratory diapausing NCR strain established prior to the introduction of Bt traits and are important for monitoring resistance evolution in NCR field populations.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Pereira AE
Division of Plant Sciences, University of Missouri, Columbia, MO.
Huynh MP
Division of Plant Sciences, University of Missouri, Columbia, MO.
Sethi A
Corteva Agriscience, Johnston, IA.
Miles AL
Corteva Agriscience, Johnston, IA.
Wade French B
North Central Agricultural Research Laboratory, Brookings, SD.
Ellersieck MR
Department of Statistics, University of Missouri, Columbia, MO.
Coudron TA
Biological Control Insect Research Laboratory, USDA-ARS, Columbia, MO.
Shelby KS
Biological Control Insect Research Laboratory, USDA-ARS, Columbia, MO.
Hibbard BE
Plant Genetics Research Unit, USDA/ARS, University of Missouri, Columbia, MO.

MeSH

AnimalsBacillus thuringiensisBacterial ProteinsColeopteraDietEndotoxinsHemolysin ProteinsLaboratoriesLarvaPest Control, BiologicalPlants, Genetically ModifiedSeedlingsZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

32789524

Citation

Pereira, Adriano E., et al. "Baseline Susceptibility of a Laboratory Strain of Northern Corn Rootworm, Diabrotica Barberi (Coleoptera: Chrysomelidae) to Bacillus Thuringiensis Traits in Seedling, Single Plant, and Diet-Toxicity Assays." Journal of Economic Entomology, vol. 113, no. 4, 2020, pp. 1955-1962.
Pereira AE, Huynh MP, Sethi A, et al. Baseline Susceptibility of a Laboratory Strain of Northern Corn Rootworm, Diabrotica barberi (Coleoptera: Chrysomelidae) to Bacillus thuringiensis Traits in Seedling, Single Plant, and Diet-Toxicity Assays. J Econ Entomol. 2020;113(4):1955-1962.
Pereira, A. E., Huynh, M. P., Sethi, A., Miles, A. L., Wade French, B., Ellersieck, M. R., Coudron, T. A., Shelby, K. S., & Hibbard, B. E. (2020). Baseline Susceptibility of a Laboratory Strain of Northern Corn Rootworm, Diabrotica barberi (Coleoptera: Chrysomelidae) to Bacillus thuringiensis Traits in Seedling, Single Plant, and Diet-Toxicity Assays. Journal of Economic Entomology, 113(4), 1955-1962. https://doi.org/10.1093/jee/toaa107
Pereira AE, et al. Baseline Susceptibility of a Laboratory Strain of Northern Corn Rootworm, Diabrotica Barberi (Coleoptera: Chrysomelidae) to Bacillus Thuringiensis Traits in Seedling, Single Plant, and Diet-Toxicity Assays. J Econ Entomol. 2020 08 13;113(4):1955-1962. PubMed PMID: 32789524.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Baseline Susceptibility of a Laboratory Strain of Northern Corn Rootworm, Diabrotica barberi (Coleoptera: Chrysomelidae) to Bacillus thuringiensis Traits in Seedling, Single Plant, and Diet-Toxicity Assays. AU - Pereira,Adriano E, AU - Huynh,Man P, AU - Sethi,Amit, AU - Miles,Ashley L, AU - Wade French,B, AU - Ellersieck,Mark R, AU - Coudron,Thomas A, AU - Shelby,Kent S, AU - Hibbard,Bruce E, PY - 2020/01/31/received PY - 2020/8/14/entrez PY - 2020/8/14/pubmed PY - 2020/12/29/medline KW - Diabrotica KW - Bt corn KW - baseline susceptibility KW - diet-toxicity assay KW - resistance management SP - 1955 EP - 1962 JF - Journal of economic entomology JO - J Econ Entomol VL - 113 IS - 4 N2 - The northern corn rootworm (NCR), Diabrotica barberi Smith & Lawrence, is an economic pest of maize in the U.S. Corn Belt. The objective of this study was to determine the baseline susceptibility of a laboratory NCR strain to Bt proteins eCry3.1Ab, mCry3A, Cry3Bb1, and Cry34/35Ab1 using seedling, single plant, and diet-toxicity assays. Plant assays were performed in greenhouse using corn hybrids expressing one of the Bt proteins and each respective near-isoline. Diet-toxicity assays, consisting of Bt proteins overlaid onto artificial diet were also conducted. In both plant assays, significantly more larvae survived Cry34/35Ab1-expressing corn compared with all other Bt-expressing corn, and larvae that survived eCry3.1Ab-expressing corn had significantly smaller head capsule widths compared with larvae that survived Cry34/35Ab1-expressing corn. In seedling assays, larvae surviving eCry3.1Ab-expressing corn also had significantly smaller head capsule widths compared with larvae that survived mCry3A-expressing corn. Additionally, larvae that survived mCry3A-expressing corn weighed significantly more than larvae surviving eCry3.1Ab- and Cry34/35Ab1-expressing corn. In single plant assays, no significant differences in larval dry weight was observed between any of the Bt-expressing corn. In diet assays, LC50s ranged from 0.14 (eCry3.1Ab) to 10.6 µg/cm2 (Cry34/35Ab1), EC50s ranged from 0.12 (Cry34/35Ab1) to 1.57 µg/cm2 (mCry3A), IC50s ranged from 0.08 (eCry3.1Ab) to 2.41 µg/cm2 (Cry34/35Ab1), and MIC50s ranged from 2.52 (eCry3.1Ab) to 14.2 µg/cm2 (mCry3A). These results establish the toxicity of four Bt proteins to a laboratory diapausing NCR strain established prior to the introduction of Bt traits and are important for monitoring resistance evolution in NCR field populations. SN - 1938-291X UR - https://www.unboundmedicine.com/medline/citation/32789524/Baseline_Susceptibility_of_a_Laboratory_Strain_of_Northern_Corn_Rootworm_Diabrotica_barberi__Coleoptera:_Chrysomelidae__to_Bacillus_thuringiensis_Traits_in_Seedling_Single_Plant_and_Diet_Toxicity_Assays_ DB - PRIME DP - Unbound Medicine ER -