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ORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection.
Nat Immunol. 2020 Oct; 21(10):1293-1301.NI

Abstract

The SARS-CoV-2 virus emerged in December 2019 and has caused a worldwide pandemic due to the lack of any pre-existing immunity. Accurate serology testing is urgently needed to help diagnose infection, determine past exposure of populations and assess the response to a future vaccine. The landscape of antibody responses to SARS-CoV-2 is unknown. In this study, we utilized the luciferase immunoprecipitation system to assess the antibody responses to 15 different SARS-CoV-2 antigens in patients with COVID-19. We identified new targets of the immune response to SARS-CoV-2 and show that nucleocapsid, open reading frame (ORF)8 and ORF3b elicit the strongest specific antibody responses. ORF8 and ORF3b antibodies, taken together as a cluster of points, identified 96.5% of COVID-19 samples at early and late time points of disease with 99.5% specificity. Our findings could be used to develop second-generation diagnostic tests to improve serological assays for COVID-19 and are important in understanding pathogenicity.

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Authors+Show Affiliations

Hachim A
HKU-Pasteur Research Pole, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Kavian N
HKU-Pasteur Research Pole, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China. niloufar@hku.hk. Faculté de Médecine Université Paris Descartes, Sorbonne Paris Cité, Assistance Publique-Hôpitaux de Paris, Hôpital Universitaire Paris Centre, Centre Hospitalier Universitaire Cochin, Service d'Immunologie Biologique, Paris, France. niloufar@hku.hk. Institut Cochin, INSERM U1016, Université Paris Descartes, Sorbonne Paris Cité, Paris, France. niloufar@hku.hk.
Cohen CA
HKU-Pasteur Research Pole, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Chin AWH
Division of Public Health Laboratory Sciences, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Chu DKW
Division of Public Health Laboratory Sciences, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Mok CKP
HKU-Pasteur Research Pole, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Tsang OTY
Infectious Diseases Centre, Princess Margaret Hospital, Hospital Authority of Hong Kong, Hong Kong SAR, China.
Yeung YC
Infectious Diseases Centre, Princess Margaret Hospital, Hospital Authority of Hong Kong, Hong Kong SAR, China.
Perera RAPM
Division of Public Health Laboratory Sciences, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Poon LLM
HKU-Pasteur Research Pole, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China. Division of Public Health Laboratory Sciences, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Peiris JSM
HKU-Pasteur Research Pole, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China. Division of Public Health Laboratory Sciences, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Valkenburg SA
HKU-Pasteur Research Pole, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China. sophiev@hku.hk.

MeSH

AdultAgedAntibodies, ViralAntigens, ViralBetacoronavirusCOVID-19COVID-19 TestingClinical Laboratory TechniquesCoronavirus InfectionsFemaleHong KongHumansMaleMiddle AgedPandemicsPneumonia, ViralSARS-CoV-2Sensitivity and SpecificityTime FactorsViral Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

32807944

Citation

Hachim, Asmaa, et al. "ORF8 and ORF3b Antibodies Are Accurate Serological Markers of Early and Late SARS-CoV-2 Infection." Nature Immunology, vol. 21, no. 10, 2020, pp. 1293-1301.
Hachim A, Kavian N, Cohen CA, et al. ORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection. Nat Immunol. 2020;21(10):1293-1301.
Hachim, A., Kavian, N., Cohen, C. A., Chin, A. W. H., Chu, D. K. W., Mok, C. K. P., Tsang, O. T. Y., Yeung, Y. C., Perera, R. A. P. M., Poon, L. L. M., Peiris, J. S. M., & Valkenburg, S. A. (2020). ORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection. Nature Immunology, 21(10), 1293-1301. https://doi.org/10.1038/s41590-020-0773-7
Hachim A, et al. ORF8 and ORF3b Antibodies Are Accurate Serological Markers of Early and Late SARS-CoV-2 Infection. Nat Immunol. 2020;21(10):1293-1301. PubMed PMID: 32807944.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection. AU - Hachim,Asmaa, AU - Kavian,Niloufar, AU - Cohen,Carolyn A, AU - Chin,Alex W H, AU - Chu,Daniel K W, AU - Mok,Chris K P, AU - Tsang,Owen T Y, AU - Yeung,Yiu Cheong, AU - Perera,Ranawaka A P M, AU - Poon,Leo L M, AU - Peiris,J S Malik, AU - Valkenburg,Sophie A, Y1 - 2020/08/17/ PY - 2020/4/27/received PY - 2020/7/30/accepted PY - 2020/8/19/pubmed PY - 2020/9/29/medline PY - 2020/8/19/entrez SP - 1293 EP - 1301 JF - Nature immunology JO - Nat Immunol VL - 21 IS - 10 N2 - The SARS-CoV-2 virus emerged in December 2019 and has caused a worldwide pandemic due to the lack of any pre-existing immunity. Accurate serology testing is urgently needed to help diagnose infection, determine past exposure of populations and assess the response to a future vaccine. The landscape of antibody responses to SARS-CoV-2 is unknown. In this study, we utilized the luciferase immunoprecipitation system to assess the antibody responses to 15 different SARS-CoV-2 antigens in patients with COVID-19. We identified new targets of the immune response to SARS-CoV-2 and show that nucleocapsid, open reading frame (ORF)8 and ORF3b elicit the strongest specific antibody responses. ORF8 and ORF3b antibodies, taken together as a cluster of points, identified 96.5% of COVID-19 samples at early and late time points of disease with 99.5% specificity. Our findings could be used to develop second-generation diagnostic tests to improve serological assays for COVID-19 and are important in understanding pathogenicity. SN - 1529-2916 UR - https://www.unboundmedicine.com/medline/citation/32807944/ORF8_and_ORF3b_antibodies_are_accurate_serological_markers_of_early_and_late_SARS_CoV_2_infection_ DB - PRIME DP - Unbound Medicine ER -