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Arrangement of Ceramides in the Skin: Sphingosine Chains Localize at a Single Position in Stratum Corneum Lipid Matrix Models.
Langmuir. 2020 09 01; 36(34):10270-10278.L

Abstract

Understanding the structure of the stratum corneum (SC) is essential to understand the skin barrier process. The long periodicity phase (LPP) is a unique trilayer lamellar structure located in the SC. Adjustments in the composition of the lipid matrix, as in many skin abnormalities, can have severe effects on the lipid organization and barrier function. Although the location of individual lipid subclasses has been identified, the lipid conformation at these locations remains uncertain. Contrast variation experiments via small-angle neutron diffraction were used to investigate the conformation of ceramide (CER) N-(tetracosanoyl)-sphingosine (NS) within both simplistic and porcine mimicking LPP models. To identify the lipid conformation of the twin chain CER NS, the chains were individually deuterated, and their scattering length profiles were calculated to identify their locations in the LPP unit cell. In the repeating trilayer unit of the LPP, the acyl chain of CER NS was located in the central and outer layers, while the sphingosine chain was located exclusively in the middle of the outer layers. Thus, for the CER NS with the acyl chain in the central layer, this demonstrates an extended conformation. Electron density distribution profiles identified that the lipid structure remains consistent regardless of the lipid's lateral packing phase, this may be partially due to the anchoring of the extended CER NS. The presented results provide a more detailed insight on the internal arrangement of the LPP lipids and how they are expected to be arranged in healthy skin.

Authors+Show Affiliations

Division of BioTherapeutics, Leiden Academic Centre for Drug Research, University of Leiden, Einsteinweg 55, Leiden 2333 CC, The Netherlands.Division of BioTherapeutics, Leiden Academic Centre for Drug Research, University of Leiden, Einsteinweg 55, Leiden 2333 CC, The Netherlands.Chemistry Department, Christopher Ingold Laboratories, University College London, London WC1H 0AJ, United Kingdom.Pharmaceutical Science Division, King's College London, London WC2R 2LS, United Kingdom.Pharmaceutical Science Division, King's College London, London WC2R 2LS, United Kingdom.Division of Pharmacy and Optometry, Manchester University, Manchester M13 9PL, United Kingdom.Institute Laue-Langevin, Grenoble 38000, France.Division of BioTherapeutics, Leiden Academic Centre for Drug Research, University of Leiden, Einsteinweg 55, Leiden 2333 CC, The Netherlands.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32816488

Citation

Beddoes, Charlotte M., et al. "Arrangement of Ceramides in the Skin: Sphingosine Chains Localize at a Single Position in Stratum Corneum Lipid Matrix Models." Langmuir : the ACS Journal of Surfaces and Colloids, vol. 36, no. 34, 2020, pp. 10270-10278.
Beddoes CM, Gooris GS, Foglia F, et al. Arrangement of Ceramides in the Skin: Sphingosine Chains Localize at a Single Position in Stratum Corneum Lipid Matrix Models. Langmuir. 2020;36(34):10270-10278.
Beddoes, C. M., Gooris, G. S., Foglia, F., Ahmadi, D., Barlow, D. J., Lawrence, M. J., Demé, B., & Bouwstra, J. A. (2020). Arrangement of Ceramides in the Skin: Sphingosine Chains Localize at a Single Position in Stratum Corneum Lipid Matrix Models. Langmuir : the ACS Journal of Surfaces and Colloids, 36(34), 10270-10278. https://doi.org/10.1021/acs.langmuir.0c01992
Beddoes CM, et al. Arrangement of Ceramides in the Skin: Sphingosine Chains Localize at a Single Position in Stratum Corneum Lipid Matrix Models. Langmuir. 2020 09 1;36(34):10270-10278. PubMed PMID: 32816488.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Arrangement of Ceramides in the Skin: Sphingosine Chains Localize at a Single Position in Stratum Corneum Lipid Matrix Models. AU - Beddoes,Charlotte M, AU - Gooris,Gert S, AU - Foglia,Fabrizia, AU - Ahmadi,Delaram, AU - Barlow,David J, AU - Lawrence,M Jayne, AU - Demé,Bruno, AU - Bouwstra,Joke A, Y1 - 2020/08/20/ PY - 2020/8/21/pubmed PY - 2021/6/22/medline PY - 2020/8/21/entrez SP - 10270 EP - 10278 JF - Langmuir : the ACS journal of surfaces and colloids JO - Langmuir VL - 36 IS - 34 N2 - Understanding the structure of the stratum corneum (SC) is essential to understand the skin barrier process. The long periodicity phase (LPP) is a unique trilayer lamellar structure located in the SC. Adjustments in the composition of the lipid matrix, as in many skin abnormalities, can have severe effects on the lipid organization and barrier function. Although the location of individual lipid subclasses has been identified, the lipid conformation at these locations remains uncertain. Contrast variation experiments via small-angle neutron diffraction were used to investigate the conformation of ceramide (CER) N-(tetracosanoyl)-sphingosine (NS) within both simplistic and porcine mimicking LPP models. To identify the lipid conformation of the twin chain CER NS, the chains were individually deuterated, and their scattering length profiles were calculated to identify their locations in the LPP unit cell. In the repeating trilayer unit of the LPP, the acyl chain of CER NS was located in the central and outer layers, while the sphingosine chain was located exclusively in the middle of the outer layers. Thus, for the CER NS with the acyl chain in the central layer, this demonstrates an extended conformation. Electron density distribution profiles identified that the lipid structure remains consistent regardless of the lipid's lateral packing phase, this may be partially due to the anchoring of the extended CER NS. The presented results provide a more detailed insight on the internal arrangement of the LPP lipids and how they are expected to be arranged in healthy skin. SN - 1520-5827 UR - https://www.unboundmedicine.com/medline/citation/32816488/Arrangement_of_Ceramides_in_the_Skin:_Sphingosine_Chains_Localize_at_a_Single_Position_in_Stratum_Corneum_Lipid_Matrix_Models_ L2 - https://doi.org/10.1021/acs.langmuir.0c01992 DB - PRIME DP - Unbound Medicine ER -