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Evaluation on testing of deep throat saliva and lower respiratory tract specimens with Xpert Xpress SARS-CoV-2 assay.
J Clin Virol. 2020 Oct; 131:104593.JC

Abstract

BACKGROUND

Xpert® Xpress SARS-CoV-2 assay is only validated on nasopharyngeal specimens for detection of SARS-CoV-2. Other specimen types such as deep throat saliva (DTS), also known as posterior oropharyngeal saliva and lower-respiratorytract specimens (LRT) including sputum, tracheal aspirate and bronchoalveolar lavage are not validated. These non-validated specimen types, however, do have significant diagnostic value.

OBJECTIVE

Evaluate the performance of Xpert Xpress SARS-CoV-2 assay for detection of SARS-CoV-2 from DTS and LRT specimens.

METHODS

162 specimens from 158 patients with suspected COVID-19 disease were tested with Xpert Xpress SARS-CoV-2 assay. These included 120 DTS and 42 LRT specimens i.e. 35 sputum, 6 tracheal aspirate and one bronchoalveolar lavage. Results were compared to those by the TIB-Molbiol LightMix® SarbecoV E-gene assay.

RESULTS

Xpert Xpress SARS-CoV-2 assay has satisfactory performance when compared with reference method. The positive percent agreement (PPA) of DTS and LRT specimens were 98.86 % & 100 % respectively while the negative percent agreement (NPA) was 100 % for both DTS and LRT specimens.

CONCLUSIONS

This study demonstrated with appropriate sample pre-treatment, Xpert Xpress SARS-CoV-2 assay can be used to test on non-validated specimen types including DTS & LRT specimens.

Authors+Show Affiliations

Department of Microbiology, Prince of Wales Hospital, Hong Kong, China. Electronic address: wcw372@ha.org.hk.Department of Microbiology, Prince of Wales Hospital, Hong Kong, China.Department of Microbiology, Prince of Wales Hospital, Hong Kong, China.Department of Microbiology, Prince of Wales Hospital, Hong Kong, China.Department of Microbiology, Prince of Wales Hospital, Hong Kong, China.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

32823131

Citation

Wong, River Chun-Wai, et al. "Evaluation On Testing of Deep Throat Saliva and Lower Respiratory Tract Specimens With Xpert Xpress SARS-CoV-2 Assay." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 131, 2020, p. 104593.
Wong RC, Wong AH, Ho YI, et al. Evaluation on testing of deep throat saliva and lower respiratory tract specimens with Xpert Xpress SARS-CoV-2 assay. J Clin Virol. 2020;131:104593.
Wong, R. C., Wong, A. H., Ho, Y. I., Leung, E. C., & Lai, R. W. (2020). Evaluation on testing of deep throat saliva and lower respiratory tract specimens with Xpert Xpress SARS-CoV-2 assay. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 131, 104593. https://doi.org/10.1016/j.jcv.2020.104593
Wong RC, et al. Evaluation On Testing of Deep Throat Saliva and Lower Respiratory Tract Specimens With Xpert Xpress SARS-CoV-2 Assay. J Clin Virol. 2020;131:104593. PubMed PMID: 32823131.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation on testing of deep throat saliva and lower respiratory tract specimens with Xpert Xpress SARS-CoV-2 assay. AU - Wong,River Chun-Wai, AU - Wong,Ann Han, AU - Ho,Yolanda Iok-Ieng, AU - Leung,Eddie Chi-Man, AU - Lai,Raymond Wai-Man, Y1 - 2020/08/16/ PY - 2020/08/07/received PY - 2020/08/10/revised PY - 2020/08/14/accepted PY - 2020/8/22/pubmed PY - 2020/10/6/medline PY - 2020/8/22/entrez KW - Deep throat saliva KW - Lower respiratory tract specimens KW - Non-validated specimen types KW - SARS-CoV-2 KW - Xpert Xpress SARS-CoV-2 assay SP - 104593 EP - 104593 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 131 N2 - BACKGROUND: Xpert® Xpress SARS-CoV-2 assay is only validated on nasopharyngeal specimens for detection of SARS-CoV-2. Other specimen types such as deep throat saliva (DTS), also known as posterior oropharyngeal saliva and lower-respiratorytract specimens (LRT) including sputum, tracheal aspirate and bronchoalveolar lavage are not validated. These non-validated specimen types, however, do have significant diagnostic value. OBJECTIVE: Evaluate the performance of Xpert Xpress SARS-CoV-2 assay for detection of SARS-CoV-2 from DTS and LRT specimens. METHODS: 162 specimens from 158 patients with suspected COVID-19 disease were tested with Xpert Xpress SARS-CoV-2 assay. These included 120 DTS and 42 LRT specimens i.e. 35 sputum, 6 tracheal aspirate and one bronchoalveolar lavage. Results were compared to those by the TIB-Molbiol LightMix® SarbecoV E-gene assay. RESULTS: Xpert Xpress SARS-CoV-2 assay has satisfactory performance when compared with reference method. The positive percent agreement (PPA) of DTS and LRT specimens were 98.86 % & 100 % respectively while the negative percent agreement (NPA) was 100 % for both DTS and LRT specimens. CONCLUSIONS: This study demonstrated with appropriate sample pre-treatment, Xpert Xpress SARS-CoV-2 assay can be used to test on non-validated specimen types including DTS & LRT specimens. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/32823131/Evaluation_on_testing_of_deep_throat_saliva_and_lower_respiratory_tract_specimens_with_Xpert_Xpress_SARS_CoV_2_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(20)30335-8 DB - PRIME DP - Unbound Medicine ER -