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Neutralizing Antibodies Correlate with Protection from SARS-CoV-2 in Humans during a Fishery Vessel Outbreak with a High Attack Rate.
J Clin Microbiol. 2020 10 21; 58(11)JC

Abstract

The development of vaccines against SARS-CoV-2 would be greatly facilitated by the identification of immunological correlates of protection in humans. However, to date, studies on protective immunity have been performed only in animal models and correlates of protection have not been established in humans. Here, we describe an outbreak of SARS-CoV-2 on a fishing vessel associated with a high attack rate. Predeparture serological and viral reverse transcription-PCR (RT-PCR) testing along with repeat testing after return to shore was available for 120 of the 122 persons on board over a median follow-up of 32.5 days (range, 18.8 to 50.5 days). A total of 104 individuals had an RT-PCR-positive viral test with a cycle threshold (CT) of <35 or seroconverted during the follow-up period, yielding an attack rate on board of 85.2% (104/122 individuals). Metagenomic sequencing of 39 viral genomes suggested that the outbreak originated largely from a single viral clade. Only three crew members tested seropositive prior to the boat's departure in initial serological screening and also had neutralizing and spike-reactive antibodies in follow-up assays. None of the crew members with neutralizing antibody titers showed evidence of bona fide viral infection or experienced any symptoms during the viral outbreak. Therefore, the presence of neutralizing antibodies from prior infection was significantly associated with protection against reinfection (Fisher's exact test, P = 0.002).

Authors+Show Affiliations

Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington, USA.Division of Basic Sciences and Computational Biology Program, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA. Department of Genome Sciences, University of Washington, Seattle, Washington, USA. Medical Scientist Training Program, University of Washington, Seattle, Washington, USA.Division of Basic Sciences and Computational Biology Program, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington, USA.Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington, USA. Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington, USA. Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington, USA. Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.Division of Basic Sciences and Computational Biology Program, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA. Department of Genome Sciences, University of Washington, Seattle, Washington, USA. Howard Hughes Medical Institute, Seattle, Washington, USA.Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington, USA agrening@uw.edu. Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

32826322

Citation

Addetia, Amin, et al. "Neutralizing Antibodies Correlate With Protection From SARS-CoV-2 in Humans During a Fishery Vessel Outbreak With a High Attack Rate." Journal of Clinical Microbiology, vol. 58, no. 11, 2020.
Addetia A, Crawford KHD, Dingens A, et al. Neutralizing Antibodies Correlate with Protection from SARS-CoV-2 in Humans during a Fishery Vessel Outbreak with a High Attack Rate. J Clin Microbiol. 2020;58(11).
Addetia, A., Crawford, K. H. D., Dingens, A., Zhu, H., Roychoudhury, P., Huang, M. L., Jerome, K. R., Bloom, J. D., & Greninger, A. L. (2020). Neutralizing Antibodies Correlate with Protection from SARS-CoV-2 in Humans during a Fishery Vessel Outbreak with a High Attack Rate. Journal of Clinical Microbiology, 58(11). https://doi.org/10.1128/JCM.02107-20
Addetia A, et al. Neutralizing Antibodies Correlate With Protection From SARS-CoV-2 in Humans During a Fishery Vessel Outbreak With a High Attack Rate. J Clin Microbiol. 2020 10 21;58(11) PubMed PMID: 32826322.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neutralizing Antibodies Correlate with Protection from SARS-CoV-2 in Humans during a Fishery Vessel Outbreak with a High Attack Rate. AU - Addetia,Amin, AU - Crawford,Katharine H D, AU - Dingens,Adam, AU - Zhu,Haiying, AU - Roychoudhury,Pavitra, AU - Huang,Meei-Li, AU - Jerome,Keith R, AU - Bloom,Jesse D, AU - Greninger,Alexander L, Y1 - 2020/10/21/ PY - 2020/08/12/received PY - 2020/08/20/accepted PY - 2020/8/23/pubmed PY - 2020/11/5/medline PY - 2020/8/23/entrez KW - COVID-19 KW - SARS-CoV-2 KW - attack rate KW - boat KW - correlates KW - fishing KW - neutralizing antibodies KW - protection KW - vessel JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 58 IS - 11 N2 - The development of vaccines against SARS-CoV-2 would be greatly facilitated by the identification of immunological correlates of protection in humans. However, to date, studies on protective immunity have been performed only in animal models and correlates of protection have not been established in humans. Here, we describe an outbreak of SARS-CoV-2 on a fishing vessel associated with a high attack rate. Predeparture serological and viral reverse transcription-PCR (RT-PCR) testing along with repeat testing after return to shore was available for 120 of the 122 persons on board over a median follow-up of 32.5 days (range, 18.8 to 50.5 days). A total of 104 individuals had an RT-PCR-positive viral test with a cycle threshold (CT) of <35 or seroconverted during the follow-up period, yielding an attack rate on board of 85.2% (104/122 individuals). Metagenomic sequencing of 39 viral genomes suggested that the outbreak originated largely from a single viral clade. Only three crew members tested seropositive prior to the boat's departure in initial serological screening and also had neutralizing and spike-reactive antibodies in follow-up assays. None of the crew members with neutralizing antibody titers showed evidence of bona fide viral infection or experienced any symptoms during the viral outbreak. Therefore, the presence of neutralizing antibodies from prior infection was significantly associated with protection against reinfection (Fisher's exact test, P = 0.002). SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/32826322/Neutralizing_Antibodies_Correlate_with_Protection_from_SARS_CoV_2_in_Humans_during_a_Fishery_Vessel_Outbreak_with_a_High_Attack_Rate_ L2 - http://jcm.asm.org/lookup/pmidlookup?view=long&amp;pmid=32826322 DB - PRIME DP - Unbound Medicine ER -