TY - JOUR T1 - Immunoinformatics-guided design of an epitope-based vaccine against severe acute respiratory syndrome coronavirus 2 spike glycoprotein. AU - Rakib,Ahmed, AU - Sami,Saad Ahmed, AU - Mimi,Nusrat Jahan, AU - Chowdhury,Md Mustafiz, AU - Eva,Taslima Akter, AU - Nainu,Firzan, AU - Paul,Arkajyoti, AU - Shahriar,Asif, AU - Tareq,Abu Montakim, AU - Emon,Nazim Uddin, AU - Chakraborty,Sajal, AU - Shil,Sagar, AU - Mily,Sabrina Jahan, AU - Ben Hadda,Taibi, AU - Almalki,Faisal A, AU - Emran,Talha Bin, Y1 - 2020/08/13/ PY - 2020/05/28/received PY - 2020/08/08/revised PY - 2020/08/08/accepted PY - 2020/8/23/pubmed PY - 2020/9/25/medline PY - 2020/8/23/entrez KW - COVID-19 KW - Epitope KW - Immunoinformatics KW - SARS-CoV-2 KW - Spike glycoprotein SP - 103967 EP - 103967 JF - Computers in biology and medicine JO - Comput Biol Med VL - 124 N2 - AIMS: With a large number of fatalities, coronavirus disease-2019 (COVID-19) has greatly affected human health worldwide. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus that causes COVID-19. The World Health Organization has declared a global pandemic of this contagious disease. Researchers across the world are collaborating in a quest for remedies to combat this deadly virus. It has recently been demonstrated that the spike glycoprotein (SGP) of SARS-CoV-2 is the mediator by which the virus enters host cells. MAIN METHODS: Our group comprehensibly analyzed the SGP of SARS-CoV-2 through multiple sequence analysis and a phylogenetic analysis. We predicted the strongest immunogenic epitopes of the SGP for both B cells and T cells. KEY FINDINGS: We focused on predicting peptides that would bind major histocompatibility complex class I. Two optimal epitopes were identified, WTAGAAAYY and GAAAYYVGY. They interact with the HLA-B*15:01 allele, which was further validated by molecular docking simulation. This study also found that the selected epitopes are able to be recognized in a large percentage of the world's population. Furthermore, we predicted CD4+ T-cell epitopes and B-cell epitopes. SIGNIFICANCE: Our study provides a strong basis for designing vaccine candidates against SARS-CoV-2. However, laboratory work is required to validate our theoretical results, which would lay the foundation for the appropriate vaccine manufacturing and testing processes. SN - 1879-0534 UR - https://www.unboundmedicine.com/medline/citation/32828069/Immunoinformatics_guided_design_of_an_epitope_based_vaccine_against_severe_acute_respiratory_syndrome_coronavirus_2_spike_glycoprotein_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0010-4825(20)30300-0 DB - PRIME DP - Unbound Medicine ER -