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Quantitative determination and validation of 17 cannabinoids in cannabis and hemp using liquid chromatography-tandem mass spectrometry.
Anal Bioanal Chem. 2020 Nov; 412(27):7381-7393.AB

Abstract

The increase in production of cannabis for medical and recreational purposes in recent years has led to a corresponding increase in laboratories performing cannabinoid analysis of cannabis and hemp. We have developed and validated a quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) method that is simple, reliable, specific, and accurate for the analysis of 17 cannabinoids in cannabis and hemp. Liquid-solid sample extraction coupled with dilution into a calibration range from 10 to 10,000 ng/mL and LC-MS/MS analysis provides quantification of samples ranging from 0.002 to 200 mg/g (0.0002 to 20.0%) in matrix. Linearity of calibration curves in methanol was demonstrated with regression r2 ≥ 0.99. Within-batch precision (0.5 to 6.5%) and accuracy (91.4 to 108.0%) and between-batch precision (0.9 to 5.1%) and accuracy (91.5 to 107.5%) were demonstrated for quality control (QC) samples in methanol. Within-batch precision (0.2 to 3.6%) and accuracy (85.4 to 111.6%) and between-batch precision (1.4 to 6.1 %) and accuracy (90.2 to 110.3%) were also evaluated with a candidate cannabis certified reference material (CRM). Repeatability (1.5 to 12.4% RSD) and intermediate precision (2.2 to 12.8% RSD) were demonstrated via analysis of seven cannabis samples with HorRat values ranging from 0.3 to 3.1. The method provides enhanced detection limits coupled with a large quantitative range for 17 cannabinoids in plant material. It is suitable for a wide range of applications including routine analysis for delta-9-tetrahydrocannabinol (Δ9-THC), delta-9-tetrahydrocannabinolic acid (Δ9-THCA), cannabidiol (CBD), cannabidiolic acid (CBDA), and cannabinol (CBN) as well as more advanced interrogation of samples for both major and minor cannabinoids.

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Authors+Show Affiliations

McRae G
National Research Council of Canada, Metrology, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada.
Melanson JE
National Research Council of Canada, Metrology, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada. Jeremy.Melanson@nrc-cnrc.gc.ca.

MeSH

CannabinoidsCannabisChromatography, High Pressure LiquidDronabinolPlant ExtractsQuality ControlTandem Mass Spectrometry

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32833075

Citation

McRae, Garnet, and Jeremy E. Melanson. "Quantitative Determination and Validation of 17 Cannabinoids in Cannabis and Hemp Using Liquid Chromatography-tandem Mass Spectrometry." Analytical and Bioanalytical Chemistry, vol. 412, no. 27, 2020, pp. 7381-7393.
McRae G, Melanson JE. Quantitative determination and validation of 17 cannabinoids in cannabis and hemp using liquid chromatography-tandem mass spectrometry. Anal Bioanal Chem. 2020;412(27):7381-7393.
McRae, G., & Melanson, J. E. (2020). Quantitative determination and validation of 17 cannabinoids in cannabis and hemp using liquid chromatography-tandem mass spectrometry. Analytical and Bioanalytical Chemistry, 412(27), 7381-7393. https://doi.org/10.1007/s00216-020-02862-8
McRae G, Melanson JE. Quantitative Determination and Validation of 17 Cannabinoids in Cannabis and Hemp Using Liquid Chromatography-tandem Mass Spectrometry. Anal Bioanal Chem. 2020;412(27):7381-7393. PubMed PMID: 32833075.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative determination and validation of 17 cannabinoids in cannabis and hemp using liquid chromatography-tandem mass spectrometry. AU - McRae,Garnet, AU - Melanson,Jeremy E, Y1 - 2020/08/24/ PY - 2020/06/29/received PY - 2020/08/04/accepted PY - 2020/07/29/revised PY - 2020/8/25/pubmed PY - 2021/5/8/medline PY - 2020/8/25/entrez KW - Cannabinoids KW - Cannabis KW - Hemp KW - Liquid chromatography-mass spectrometry KW - Validation SP - 7381 EP - 7393 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 412 IS - 27 N2 - The increase in production of cannabis for medical and recreational purposes in recent years has led to a corresponding increase in laboratories performing cannabinoid analysis of cannabis and hemp. We have developed and validated a quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) method that is simple, reliable, specific, and accurate for the analysis of 17 cannabinoids in cannabis and hemp. Liquid-solid sample extraction coupled with dilution into a calibration range from 10 to 10,000 ng/mL and LC-MS/MS analysis provides quantification of samples ranging from 0.002 to 200 mg/g (0.0002 to 20.0%) in matrix. Linearity of calibration curves in methanol was demonstrated with regression r2 ≥ 0.99. Within-batch precision (0.5 to 6.5%) and accuracy (91.4 to 108.0%) and between-batch precision (0.9 to 5.1%) and accuracy (91.5 to 107.5%) were demonstrated for quality control (QC) samples in methanol. Within-batch precision (0.2 to 3.6%) and accuracy (85.4 to 111.6%) and between-batch precision (1.4 to 6.1 %) and accuracy (90.2 to 110.3%) were also evaluated with a candidate cannabis certified reference material (CRM). Repeatability (1.5 to 12.4% RSD) and intermediate precision (2.2 to 12.8% RSD) were demonstrated via analysis of seven cannabis samples with HorRat values ranging from 0.3 to 3.1. The method provides enhanced detection limits coupled with a large quantitative range for 17 cannabinoids in plant material. It is suitable for a wide range of applications including routine analysis for delta-9-tetrahydrocannabinol (Δ9-THC), delta-9-tetrahydrocannabinolic acid (Δ9-THCA), cannabidiol (CBD), cannabidiolic acid (CBDA), and cannabinol (CBN) as well as more advanced interrogation of samples for both major and minor cannabinoids. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/32833075/Quantitative_determination_and_validation_of_17_cannabinoids_in_cannabis_and_hemp_using_liquid_chromatography_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -