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Functional mapping of B-cell linear epitopes of SARS-CoV-2 in COVID-19 convalescent population.Emerg Microbes Infect. 2020 Dec; 9(1):1988-1996.EM
Abstract
ABSTRACT
Pandemic SARS-CoV-2 has caused unprecedented mortalities. Vaccine is in urgent need to stop the pandemic. Despite great progresses on SARS-CoV-2 vaccine development, the efficacy of the vaccines remains to be determined. Deciphering the interactions of the viral epitopes with the elicited neutralizing antibodies in convalescent population inspires the vaccine development. In this study, we devised a peptide array composed of 20-mer overlapped peptides of spike (S), membrane (M) and envelope (E) proteins, and performed a screening with 120 COVID-19 convalescent sera and 24 non-COVID-19 sera. We identified five SARS-CoV-2-specific dominant epitopes that reacted with above 40% COVID-19 convalescent sera. Of note, two peptides non-specifically interacted with most of the non-COVID-19 sera. Neutralization assay indicated that only five sera completely blocked viral infection at the dilution of 1:200. By using a peptide-compete neutralizing assay, we found that three dominant epitopes partially competed the neutralization activity of several convalescent sera, suggesting antibodies elicited by these epitopes played an important role in neutralizing viral infection. The epitopes we identified in this study may serve as vaccine candidates to elicit neutralizing antibodies in most vaccinated people or specific antigens for SARS-CoV-2 diagnosis.Links
MeSH
Pub Type(s)
Journal Article
Language
eng
PubMed ID
32844713
Citation
Yi, Zhigang, et al. "Functional Mapping of B-cell Linear Epitopes of SARS-CoV-2 in COVID-19 Convalescent Population." Emerging Microbes & Infections, vol. 9, no. 1, 2020, pp. 1988-1996.
Yi Z, Ling Y, Zhang X, et al. Functional mapping of B-cell linear epitopes of SARS-CoV-2 in COVID-19 convalescent population. Emerg Microbes Infect. 2020;9(1):1988-1996.
Yi, Z., Ling, Y., Zhang, X., Chen, J., Hu, K., Wang, Y., Song, W., Ying, T., Zhang, R., Lu, H., & Yuan, Z. (2020). Functional mapping of B-cell linear epitopes of SARS-CoV-2 in COVID-19 convalescent population. Emerging Microbes & Infections, 9(1), 1988-1996. https://doi.org/10.1080/22221751.2020.1815591
Yi Z, et al. Functional Mapping of B-cell Linear Epitopes of SARS-CoV-2 in COVID-19 Convalescent Population. Emerg Microbes Infect. 2020;9(1):1988-1996. PubMed PMID: 32844713.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Functional mapping of B-cell linear epitopes of SARS-CoV-2 in COVID-19 convalescent population.
AU - Yi,Zhigang,
AU - Ling,Yun,
AU - Zhang,Xiaonan,
AU - Chen,Jieliang,
AU - Hu,Kongying,
AU - Wang,Yuyan,
AU - Song,Wuhui,
AU - Ying,Tianlei,
AU - Zhang,Rong,
AU - Lu,HongZhou,
AU - Yuan,Zhenghong,
PY - 2020/8/28/pubmed
PY - 2020/9/29/medline
PY - 2020/8/27/entrez
KW - COVID-19
KW - SARS-CoV-2
KW - diagnosis
KW - epitope
KW - neutralizing antibody
KW - vaccine
SP - 1988
EP - 1996
JF - Emerging microbes & infections
JO - Emerg Microbes Infect
VL - 9
IS - 1
N2 - ABSTRACT Pandemic SARS-CoV-2 has caused unprecedented mortalities. Vaccine is in urgent need to stop the pandemic. Despite great progresses on SARS-CoV-2 vaccine development, the efficacy of the vaccines remains to be determined. Deciphering the interactions of the viral epitopes with the elicited neutralizing antibodies in convalescent population inspires the vaccine development. In this study, we devised a peptide array composed of 20-mer overlapped peptides of spike (S), membrane (M) and envelope (E) proteins, and performed a screening with 120 COVID-19 convalescent sera and 24 non-COVID-19 sera. We identified five SARS-CoV-2-specific dominant epitopes that reacted with above 40% COVID-19 convalescent sera. Of note, two peptides non-specifically interacted with most of the non-COVID-19 sera. Neutralization assay indicated that only five sera completely blocked viral infection at the dilution of 1:200. By using a peptide-compete neutralizing assay, we found that three dominant epitopes partially competed the neutralization activity of several convalescent sera, suggesting antibodies elicited by these epitopes played an important role in neutralizing viral infection. The epitopes we identified in this study may serve as vaccine candidates to elicit neutralizing antibodies in most vaccinated people or specific antigens for SARS-CoV-2 diagnosis.
SN - 2222-1751
UR - https://www.unboundmedicine.com/medline/citation/32844713/Functional_mapping_of_B_cell_linear_epitopes_of_SARS_CoV_2_in_COVID_19_convalescent_population_
L2 - https://doi.org/10.1080/22221751.2020.1815591
DB - PRIME
DP - Unbound Medicine
ER -