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Comparative metabolite profiling and antioxidant potentials of seeds and sprouts of three Egyptian cultivars of Vicia faba L.
Food Res Int. 2020 10; 136:109537.FR

Abstract

Fava beans are considered basic food in Egypt and all over the world. They are a source of many bioactive secondary metabolites. Since there are many Egyptian cultivars of fava beans, this study addresses comparative chemical profiling of three Egyptian cultivars of fava beans namely Sakha 3, Nubaria 3, and Giza 843. The study also focuses on the effect of germination on the chemical profile of phenolic compounds and saponins. Sixty-five metabolites were characterized based on their UV spectra, accurate MS, and MS/MS data using RP-HPLC-DAD-ESI-QTOF-MS, including 37 metabolites reported for the first time in fava beans. The observed metabolites are phenolic compounds (flavonoids and phenolic acids), saponins, alkaloids, amino acids, nucleosides, organic acids, sugars, and a jasmonate. In fact, upon quantifying the area of metabolites in their chromatographic profiles, it was remarkable that germination increased the quantities of flavonoids, phenolic acids, and saponins. Moreover, the yield (%) of extracts, TPC, and DPPH increased upon sprouting. The TPC and the relative amounts of flavones, saponins, and amino acids showed a good positive correlation with DPPH. These findings elucidated the first report on the metabolic profiling of these cultivars as well as their antioxidant activities.

Authors+Show Affiliations

Department of Pharmacognosy, Faculty of Pharmacy, Egyptian Russian University, Badr City, Cairo-Suez Road, 11829 Cairo, Egypt; Research and Development Functional Food Centre (CIDAF), Bioregiόn Building, Health Science Technological Park, Avenida del Conocimiento s/n, 18016 Granada, Spain. Electronic address: reham-mekky@eru.edu.eg.Department of Pharmacognosy, Faculty of Pharmacy, Egyptian Russian University, Badr City, Cairo-Suez Road, 11829 Cairo, Egypt.Research and Development Functional Food Centre (CIDAF), Bioregiόn Building, Health Science Technological Park, Avenida del Conocimiento s/n, 18016 Granada, Spain; Department of Analytical Chemistry, Faculty of Sciences, University of Granada, AvenidaFuentenueva s/n, 18071 Granada, Spain.Department of Pharmacognosy, Faculty of Pharmacy, Egyptian Russian University, Badr City, Cairo-Suez Road, 11829 Cairo, Egypt.Department of Pharmacognosy, Faculty of Pharmacy, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt.Research and Development Functional Food Centre (CIDAF), Bioregiόn Building, Health Science Technological Park, Avenida del Conocimiento s/n, 18016 Granada, Spain; Department of Analytical Chemistry, Faculty of Sciences, University of Granada, AvenidaFuentenueva s/n, 18071 Granada, Spain.Department of Pharmacognosy, Faculty of Pharmacy, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt. Electronic address: essam.abdelsattar@pharma.cu.edu.eg.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32846596

Citation

Mekky, Reham Hassan, et al. "Comparative Metabolite Profiling and Antioxidant Potentials of Seeds and Sprouts of Three Egyptian Cultivars of Vicia Faba L." Food Research International (Ottawa, Ont.), vol. 136, 2020, p. 109537.
Mekky RH, Thabet MM, Rodríguez-Pérez C, et al. Comparative metabolite profiling and antioxidant potentials of seeds and sprouts of three Egyptian cultivars of Vicia faba L. Food Res Int. 2020;136:109537.
Mekky, R. H., Thabet, M. M., Rodríguez-Pérez, C., Elnaggar, D. M. Y., Mahrous, E. A., Segura-Carretero, A., & Abdel-Sattar, E. (2020). Comparative metabolite profiling and antioxidant potentials of seeds and sprouts of three Egyptian cultivars of Vicia faba L. Food Research International (Ottawa, Ont.), 136, 109537. https://doi.org/10.1016/j.foodres.2020.109537
Mekky RH, et al. Comparative Metabolite Profiling and Antioxidant Potentials of Seeds and Sprouts of Three Egyptian Cultivars of Vicia Faba L. Food Res Int. 2020;136:109537. PubMed PMID: 32846596.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative metabolite profiling and antioxidant potentials of seeds and sprouts of three Egyptian cultivars of Vicia faba L. AU - Mekky,Reham Hassan, AU - Thabet,Mareena M, AU - Rodríguez-Pérez,Celia, AU - Elnaggar,Dina M Yousry, AU - Mahrous,Engy A, AU - Segura-Carretero,Antonio, AU - Abdel-Sattar,Essam, Y1 - 2020/07/18/ PY - 2019/10/23/received PY - 2020/07/03/revised PY - 2020/07/08/accepted PY - 2020/8/28/entrez PY - 2020/8/28/pubmed PY - 2021/5/15/medline KW - Fava beans (Vicia faba L.) KW - HPLC-DAD-ESI-QTOF-MS/MS KW - Phenolics KW - Saponins KW - Sprouts SP - 109537 EP - 109537 JF - Food research international (Ottawa, Ont.) JO - Food Res Int VL - 136 N2 - Fava beans are considered basic food in Egypt and all over the world. They are a source of many bioactive secondary metabolites. Since there are many Egyptian cultivars of fava beans, this study addresses comparative chemical profiling of three Egyptian cultivars of fava beans namely Sakha 3, Nubaria 3, and Giza 843. The study also focuses on the effect of germination on the chemical profile of phenolic compounds and saponins. Sixty-five metabolites were characterized based on their UV spectra, accurate MS, and MS/MS data using RP-HPLC-DAD-ESI-QTOF-MS, including 37 metabolites reported for the first time in fava beans. The observed metabolites are phenolic compounds (flavonoids and phenolic acids), saponins, alkaloids, amino acids, nucleosides, organic acids, sugars, and a jasmonate. In fact, upon quantifying the area of metabolites in their chromatographic profiles, it was remarkable that germination increased the quantities of flavonoids, phenolic acids, and saponins. Moreover, the yield (%) of extracts, TPC, and DPPH increased upon sprouting. The TPC and the relative amounts of flavones, saponins, and amino acids showed a good positive correlation with DPPH. These findings elucidated the first report on the metabolic profiling of these cultivars as well as their antioxidant activities. SN - 1873-7145 UR - https://www.unboundmedicine.com/medline/citation/32846596/Comparative_metabolite_profiling_and_antioxidant_potentials_of_seeds_and_sprouts_of_three_Egyptian_cultivars_of_Vicia_faba_L_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0963-9969(20)30562-7 DB - PRIME DP - Unbound Medicine ER -